Experiment: GFP_control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glmM and folP overlap by 8 nucleotides folP and ftsH are separated by 89 nucleotides ftsH and rlmE are separated by 99 nucleotides rlmE and yhbY are separated by 125 nucleotides
b3176: glmM - phosphoglucosamine mutase (NCBI), at 3,320,755 to 3,322,092
glmM
b3177: folP - 7,8-dihydropteroate synthase (VIMSS), at 3,322,085 to 3,322,933
folP
b3178: ftsH - protease, ATP-dependent zinc-metallo (NCBI), at 3,323,023 to 3,324,957
ftsH
b3179: rlmE - 23S rRNA U2552 ribose 2'-O-methyltransferase, SAM-dependent (NCBI), at 3,325,057 to 3,325,686
rlmE
b3180: yhbY - predicted RNA-binding protein (NCBI), at 3,325,812 to 3,326,105
yhbY
Position (kb)
3323
3324
3325 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3322.094 kb on - strand at 3322.095 kb on - strand at 3322.096 kb on - strand at 3322.096 kb on - strand at 3322.115 kb on - strand at 3322.944 kb on - strand at 3322.944 kb on - strand at 3323.027 kb on - strand at 3323.048 kb on - strand at 3323.048 kb on - strand at 3323.059 kb on - strand at 3323.059 kb on - strand at 3323.059 kb on - strand at 3323.074 kb on - strand at 3323.092 kb on - strand at 3323.096 kb on - strand at 3323.105 kb on - strand at 3323.105 kb on - strand at 3323.168 kb on - strand at 3324.988 kb on - strand at 3324.988 kb on - strand at 3325.020 kb on - strand at 3325.021 kb on - strand at 3325.022 kb on - strand at 3325.025 kb on - strand at 3325.061 kb on + strand at 3325.061 kb on + strand at 3325.064 kb on - strand at 3325.064 kb on - strand at 3325.085 kb on - strand at 3325.085 kb on - strand at 3325.749 kb on - strand at 3325.849 kb on + strand, within yhbY at 3325.849 kb on + strand at 3325.883 kb on - strand, within yhbY at 3325.908 kb on - strand, within yhbY at 3325.913 kb on + strand, within yhbY at 3325.921 kb on - strand, within yhbY at 3325.928 kb on + strand, within yhbY at 3325.932 kb on + strand, within yhbY at 3325.939 kb on + strand at 3325.940 kb on - strand, within yhbY at 3325.940 kb on - strand, within yhbY
Per-strain Table
Position Strand Gene LocusTag Fraction GFP_control remove 3,322,094 - +0.8 3,322,095 - +0.7 3,322,096 - -3.2 3,322,096 - -2.9 3,322,115 - +0.4 3,322,944 - +0.1 3,322,944 - +1.0 3,323,027 - -0.0 3,323,048 - -0.1 3,323,048 - -1.6 3,323,059 - -0.2 3,323,059 - -2.3 3,323,059 - -3.9 3,323,074 - -1.7 3,323,092 - +1.9 3,323,096 - -0.2 3,323,105 - +0.2 3,323,105 - -0.8 3,323,168 - -0.3 3,324,988 - -1.5 3,324,988 - -5.6 3,325,020 - +0.6 3,325,021 - -1.2 3,325,022 - +1.0 3,325,025 - -1.7 3,325,061 + +1.9 3,325,061 + -0.4 3,325,064 - +0.6 3,325,064 - -0.3 3,325,085 - -1.7 3,325,085 - -1.8 3,325,749 - +0.4 3,325,849 + yhbY b3180 0.13 -2.4 3,325,849 + -0.7 3,325,883 - yhbY b3180 0.24 +1.0 3,325,908 - yhbY b3180 0.33 -5.1 3,325,913 + yhbY b3180 0.34 -2.5 3,325,921 - yhbY b3180 0.37 -0.2 3,325,928 + yhbY b3180 0.39 -2.2 3,325,932 + yhbY b3180 0.41 -0.7 3,325,939 + -0.1 3,325,940 - yhbY b3180 0.44 +0.5 3,325,940 - yhbY b3180 0.44 +0.3
Or see this region's nucleotide sequence