Experiment: GFP_control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phnI and phnH overlap by 1 nucleotides phnH and phnG overlap by 4 nucleotides phnG and phnF are separated by 0 nucleotides phnF and phnE are separated by 20 nucleotides
b4099: phnI - carbon-phosphorus lyase complex subunit (NCBI), at 4,317,622 to 4,318,686
phnI
b4100: phnH - carbon-phosphorus lyase complex subunit (NCBI), at 4,318,686 to 4,319,270
phnH
b4101: phnG - carbon-phosphorus lyase complex subunit (NCBI), at 4,319,267 to 4,319,719
phnG
b4102: phnF - predicted DNA-binding transcriptional regulator of phosphonate uptake and biodegradation (NCBI), at 4,319,720 to 4,320,445
phnF
b4104: phnE - phosphonate/organophosphate ester transporter subunit (NCBI), at 4,320,466 to 4,320,837
phnE
Position (kb) 4319
4320 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4318.321 kb on - strand, within phnI at 4318.326 kb on - strand, within phnI at 4318.392 kb on + strand, within phnI at 4318.400 kb on + strand, within phnI at 4318.489 kb on - strand, within phnI at 4318.549 kb on + strand, within phnI at 4318.635 kb on - strand at 4318.635 kb on - strand at 4318.687 kb on - strand at 4318.702 kb on - strand at 4318.748 kb on - strand, within phnH at 4318.799 kb on - strand, within phnH at 4318.814 kb on + strand, within phnH at 4318.855 kb on + strand, within phnH at 4318.855 kb on + strand, within phnH at 4319.165 kb on + strand, within phnH at 4319.223 kb on + strand at 4319.223 kb on + strand at 4319.223 kb on + strand at 4319.228 kb on + strand at 4319.258 kb on + strand at 4319.258 kb on + strand at 4319.259 kb on + strand at 4319.274 kb on - strand at 4319.279 kb on - strand at 4319.279 kb on - strand at 4319.354 kb on - strand, within phnG at 4319.366 kb on - strand, within phnG at 4319.379 kb on - strand, within phnG at 4319.434 kb on - strand, within phnG at 4319.446 kb on - strand, within phnG at 4319.573 kb on + strand, within phnG at 4319.731 kb on - strand at 4319.731 kb on - strand at 4319.803 kb on + strand, within phnF at 4319.803 kb on + strand, within phnF at 4319.803 kb on + strand, within phnF at 4319.811 kb on - strand, within phnF at 4319.811 kb on - strand, within phnF at 4319.985 kb on - strand, within phnF at 4319.999 kb on + strand, within phnF at 4320.073 kb on - strand, within phnF at 4320.121 kb on - strand, within phnF at 4320.253 kb on - strand, within phnF at 4320.253 kb on - strand, within phnF at 4320.292 kb on + strand, within phnF at 4320.326 kb on + strand, within phnF at 4320.348 kb on - strand, within phnF at 4320.405 kb on - strand at 4320.412 kb on + strand at 4320.412 kb on + strand at 4320.652 kb on - strand, within phnE at 4320.670 kb on - strand, within phnE at 4320.678 kb on - strand, within phnE at 4320.691 kb on + strand, within phnE
Per-strain Table
Position Strand Gene LocusTag Fraction GFP_control remove 4,318,321 - phnI b4099 0.66 +0.4 4,318,326 - phnI b4099 0.66 +1.9 4,318,392 + phnI b4099 0.72 +0.5 4,318,400 + phnI b4099 0.73 +1.1 4,318,489 - phnI b4099 0.81 +0.5 4,318,549 + phnI b4099 0.87 +0.6 4,318,635 - -0.4 4,318,635 - +1.0 4,318,687 - +0.2 4,318,702 - -1.2 4,318,748 - phnH b4100 0.11 +0.1 4,318,799 - phnH b4100 0.19 +1.5 4,318,814 + phnH b4100 0.22 +1.3 4,318,855 + phnH b4100 0.29 +2.2 4,318,855 + phnH b4100 0.29 -1.9 4,319,165 + phnH b4100 0.82 +0.3 4,319,223 + +1.1 4,319,223 + +2.2 4,319,223 + +0.5 4,319,228 + +1.4 4,319,258 + +1.4 4,319,258 + +0.6 4,319,259 + +1.4 4,319,274 - +0.6 4,319,279 - +2.4 4,319,279 - +1.1 4,319,354 - phnG b4101 0.19 +0.6 4,319,366 - phnG b4101 0.22 -3.0 4,319,379 - phnG b4101 0.25 -0.6 4,319,434 - phnG b4101 0.37 -1.1 4,319,446 - phnG b4101 0.40 +0.2 4,319,573 + phnG b4101 0.68 +1.8 4,319,731 - +1.8 4,319,731 - -0.4 4,319,803 + phnF b4102 0.11 -2.4 4,319,803 + phnF b4102 0.11 -1.5 4,319,803 + phnF b4102 0.11 -0.2 4,319,811 - phnF b4102 0.13 +0.6 4,319,811 - phnF b4102 0.13 -3.2 4,319,985 - phnF b4102 0.37 -2.9 4,319,999 + phnF b4102 0.38 -1.4 4,320,073 - phnF b4102 0.49 -3.3 4,320,121 - phnF b4102 0.55 -0.7 4,320,253 - phnF b4102 0.73 -1.9 4,320,253 - phnF b4102 0.73 -1.7 4,320,292 + phnF b4102 0.79 -1.2 4,320,326 + phnF b4102 0.83 +0.4 4,320,348 - phnF b4102 0.87 -0.9 4,320,405 - -1.4 4,320,412 + -0.8 4,320,412 + -1.7 4,320,652 - phnE b4104 0.50 -0.6 4,320,670 - phnE b4104 0.55 +0.5 4,320,678 - phnE b4104 0.57 -2.9 4,320,691 + phnE b4104 0.60 -1.0
Or see this region's nucleotide sequence