Strain Fitness in Escherichia coli BW25113 around b3294

Experiment: GFP_control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyhdL and zntR are separated by 55 nucleotideszntR and yhdN are separated by 10 nucleotidesyhdN and rplQ are separated by 106 nucleotidesrplQ and rpoA are separated by 40 nucleotides b4550: yhdL - hypothetical protein (NCBI), at 3,436,453 to 3,436,671 yhdL b3292: zntR - zinc-responsive transcriptional regulator (NCBI), at 3,436,727 to 3,437,152 zntR b3293: yhdN - hypothetical protein (NCBI), at 3,437,163 to 3,437,531 yhdN b3294: rplQ - 50S ribosomal protein L17 (NCBI), at 3,437,638 to 3,438,021 rplQ b3295: rpoA - DNA-directed RNA polymerase subunit alpha (NCBI), at 3,438,062 to 3,439,051 rpoA Position (kb) 3437 3438 3439Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3436.642 kb on - strand, within yhdLat 3436.644 kb on + strand, within yhdLat 3436.659 kb on + strandat 3436.748 kb on + strandat 3436.757 kb on + strandat 3436.767 kb on + strandat 3436.796 kb on - strand, within zntRat 3436.803 kb on + strand, within zntRat 3436.811 kb on - strand, within zntRat 3436.811 kb on - strand, within zntRat 3436.840 kb on + strand, within zntRat 3436.840 kb on + strand, within zntRat 3436.848 kb on + strand, within zntRat 3436.889 kb on + strand, within zntRat 3436.889 kb on + strand, within zntRat 3436.927 kb on + strand, within zntRat 3436.935 kb on - strand, within zntRat 3436.935 kb on - strand, within zntRat 3436.987 kb on - strand, within zntRat 3436.994 kb on - strand, within zntRat 3436.994 kb on - strand, within zntRat 3437.070 kb on - strand, within zntRat 3437.111 kb on - strandat 3437.111 kb on - strandat 3437.158 kb on - strandat 3437.158 kb on - strandat 3437.166 kb on - strandat 3437.223 kb on + strand, within yhdNat 3437.226 kb on + strand, within yhdNat 3437.234 kb on - strand, within yhdNat 3437.276 kb on + strand, within yhdNat 3437.338 kb on + strand, within yhdNat 3437.425 kb on - strand, within yhdNat 3437.425 kb on - strand, within yhdNat 3437.492 kb on + strand, within yhdNat 3437.500 kb on - strandat 3437.500 kb on - strandat 3437.502 kb on - strandat 3437.504 kb on + strandat 3437.529 kb on + strandat 3437.534 kb on - strandat 3437.669 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction GFP_control
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3,436,642 - yhdL b4550 0.86 -0.4
3,436,644 + yhdL b4550 0.87 +0.1
3,436,659 + -0.4
3,436,748 + -0.1
3,436,757 + -3.1
3,436,767 + -0.2
3,436,796 - zntR b3292 0.16 +1.0
3,436,803 + zntR b3292 0.18 -2.6
3,436,811 - zntR b3292 0.20 -2.0
3,436,811 - zntR b3292 0.20 -2.7
3,436,840 + zntR b3292 0.27 +1.2
3,436,840 + zntR b3292 0.27 -0.1
3,436,848 + zntR b3292 0.28 -0.7
3,436,889 + zntR b3292 0.38 -2.8
3,436,889 + zntR b3292 0.38 -0.2
3,436,927 + zntR b3292 0.47 -1.9
3,436,935 - zntR b3292 0.49 -2.7
3,436,935 - zntR b3292 0.49 +0.6
3,436,987 - zntR b3292 0.61 -1.3
3,436,994 - zntR b3292 0.63 +0.7
3,436,994 - zntR b3292 0.63 -0.1
3,437,070 - zntR b3292 0.81 +1.2
3,437,111 - -0.1
3,437,111 - -1.2
3,437,158 - -4.2
3,437,158 - -0.2
3,437,166 - -0.2
3,437,223 + yhdN b3293 0.16 -1.5
3,437,226 + yhdN b3293 0.17 -0.9
3,437,234 - yhdN b3293 0.19 +1.1
3,437,276 + yhdN b3293 0.31 -3.6
3,437,338 + yhdN b3293 0.47 -0.5
3,437,425 - yhdN b3293 0.71 -0.9
3,437,425 - yhdN b3293 0.71 +1.2
3,437,492 + yhdN b3293 0.89 -2.8
3,437,500 - +0.9
3,437,500 - -0.1
3,437,502 - +0.2
3,437,504 + -1.9
3,437,529 + -0.7
3,437,534 - -0.1
3,437,669 - -2.8

Or see this region's nucleotide sequence