Experiment: GFP_control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt umuC and dsbB are separated by 45 nucleotides dsbB and nhaB are separated by 145 nucleotides nhaB and fadR are separated by 220 nucleotides fadR and ycgB are separated by 51 nucleotides
b1184: umuC - DNA polymerase V subunit UmuC (NCBI), at 1,230,409 to 1,231,677
umuC
b1185: dsbB - reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS), at 1,231,723 to 1,232,253
dsbB
b1186: nhaB - NhaB sodium/proton transporter (Katherine Huang), at 1,232,399 to 1,233,940
nhaB
b1187: fadR - fatty acid metabolism regulator (NCBI), at 1,234,161 to 1,234,880
fadR
b1188: ycgB - ycgB component of nitrogen-related signalling system (of yeaGH-ycgB) (from data) , at 1,234,932 to 1,236,464
ycgB
Position (kb)
1232
1233
1234 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 1231.457 kb on + strand, within umuC at 1231.519 kb on - strand, within umuC at 1231.550 kb on + strand, within umuC at 1231.551 kb on - strand at 1231.553 kb on - strand at 1231.573 kb on - strand at 1231.597 kb on + strand at 1231.625 kb on + strand at 1231.627 kb on - strand at 1231.643 kb on - strand at 1231.645 kb on - strand at 1231.660 kb on - strand at 1231.961 kb on + strand, within dsbB at 1231.961 kb on + strand, within dsbB at 1231.979 kb on - strand, within dsbB at 1232.074 kb on + strand, within dsbB at 1232.074 kb on + strand, within dsbB at 1232.077 kb on - strand, within dsbB at 1232.272 kb on + strand at 1232.272 kb on + strand at 1232.504 kb on - strand at 1232.509 kb on - strand at 1232.525 kb on + strand at 1232.569 kb on - strand, within nhaB at 1232.784 kb on - strand, within nhaB at 1232.784 kb on - strand, within nhaB at 1232.921 kb on - strand, within nhaB at 1232.921 kb on - strand, within nhaB at 1232.936 kb on + strand, within nhaB at 1233.026 kb on + strand, within nhaB at 1233.087 kb on - strand, within nhaB at 1233.196 kb on - strand, within nhaB at 1233.269 kb on + strand, within nhaB at 1233.277 kb on - strand, within nhaB at 1233.310 kb on - strand, within nhaB at 1233.518 kb on + strand, within nhaB at 1233.518 kb on + strand, within nhaB at 1233.521 kb on - strand, within nhaB at 1233.652 kb on - strand, within nhaB at 1233.686 kb on + strand, within nhaB at 1233.723 kb on - strand, within nhaB at 1233.736 kb on - strand, within nhaB at 1233.736 kb on - strand, within nhaB at 1233.764 kb on - strand, within nhaB at 1233.764 kb on - strand, within nhaB at 1233.923 kb on + strand at 1233.923 kb on + strand at 1233.931 kb on - strand at 1234.004 kb on - strand at 1234.099 kb on - strand at 1234.102 kb on + strand at 1234.112 kb on + strand at 1234.114 kb on + strand at 1234.118 kb on - strand at 1234.118 kb on - strand at 1234.157 kb on + strand at 1234.222 kb on + strand at 1234.299 kb on + strand, within fadR at 1234.317 kb on - strand, within fadR at 1234.331 kb on + strand, within fadR at 1234.436 kb on - strand at 1234.565 kb on + strand, within fadR at 1234.673 kb on + strand, within fadR at 1234.681 kb on - strand, within fadR at 1234.720 kb on + strand, within fadR at 1234.807 kb on + strand, within fadR at 1234.807 kb on + strand, within fadR at 1234.815 kb on - strand at 1234.889 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction GFP_control remove 1,231,457 + umuC b1184 0.83 +1.4 1,231,519 - umuC b1184 0.87 +1.0 1,231,550 + umuC b1184 0.90 +1.2 1,231,551 - +0.2 1,231,553 - +1.6 1,231,573 - -0.7 1,231,597 + +0.5 1,231,625 + +1.3 1,231,627 - -1.4 1,231,643 - -0.3 1,231,645 - +1.9 1,231,660 - -1.7 1,231,961 + dsbB b1185 0.45 -1.6 1,231,961 + dsbB b1185 0.45 -0.4 1,231,979 - dsbB b1185 0.48 -2.7 1,232,074 + dsbB b1185 0.66 -1.9 1,232,074 + dsbB b1185 0.66 -0.7 1,232,077 - dsbB b1185 0.67 -1.7 1,232,272 + +1.7 1,232,272 + +0.8 1,232,504 - -1.8 1,232,509 - +0.4 1,232,525 + +0.0 1,232,569 - nhaB b1186 0.11 -2.4 1,232,784 - nhaB b1186 0.25 -0.4 1,232,784 - nhaB b1186 0.25 -0.0 1,232,921 - nhaB b1186 0.34 -2.1 1,232,921 - nhaB b1186 0.34 -1.7 1,232,936 + nhaB b1186 0.35 -3.6 1,233,026 + nhaB b1186 0.41 -1.8 1,233,087 - nhaB b1186 0.45 -0.4 1,233,196 - nhaB b1186 0.52 -2.6 1,233,269 + nhaB b1186 0.56 -5.2 1,233,277 - nhaB b1186 0.57 -0.5 1,233,310 - nhaB b1186 0.59 -0.4 1,233,518 + nhaB b1186 0.73 +0.2 1,233,518 + nhaB b1186 0.73 -0.4 1,233,521 - nhaB b1186 0.73 -0.2 1,233,652 - nhaB b1186 0.81 -0.5 1,233,686 + nhaB b1186 0.83 +0.4 1,233,723 - nhaB b1186 0.86 -0.9 1,233,736 - nhaB b1186 0.87 +0.0 1,233,736 - nhaB b1186 0.87 +0.8 1,233,764 - nhaB b1186 0.89 -1.9 1,233,764 - nhaB b1186 0.89 -1.1 1,233,923 + +0.3 1,233,923 + -0.4 1,233,931 - -0.7 1,234,004 - +0.9 1,234,099 - +1.8 1,234,102 + +1.3 1,234,112 + -5.2 1,234,114 + -0.1 1,234,118 - +0.5 1,234,118 - -3.6 1,234,157 + -1.6 1,234,222 + -3.9 1,234,299 + fadR b1187 0.19 -2.2 1,234,317 - fadR b1187 0.22 -2.1 1,234,331 + fadR b1187 0.24 -2.7 1,234,436 - -1.1 1,234,565 + fadR b1187 0.56 -6.0 1,234,673 + fadR b1187 0.71 -3.7 1,234,681 - fadR b1187 0.72 -2.5 1,234,720 + fadR b1187 0.78 -2.4 1,234,807 + fadR b1187 0.90 -3.3 1,234,807 + fadR b1187 0.90 -3.0 1,234,815 - -2.8 1,234,889 - -1.3
Or see this region's nucleotide sequence