Strain Fitness in Escherichia coli BW25113 around b0591

Experiment: GFP_control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfepD and ybdA are separated by 110 nucleotidesybdA and fepB are separated by 3 nucleotides b0590: fepD - iron-enterobactin transporter subunit (NCBI), at 620,408 to 621,412 fepD b0591: ybdA - predicted transporter (NCBI), at 621,523 to 622,773 ybdA b0592: fepB - iron-enterobactin transporter subunit (NCBI), at 622,777 to 623,733 fepB Position (kb) 621 622 623Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 620.634 kb on - strand, within fepDat 620.653 kb on - strand, within fepDat 620.653 kb on - strand, within fepDat 620.664 kb on - strand, within fepDat 620.672 kb on - strand, within fepDat 620.728 kb on - strand, within fepDat 620.749 kb on + strand, within fepDat 620.749 kb on + strand, within fepDat 620.749 kb on + strand, within fepDat 620.780 kb on - strand, within fepDat 620.808 kb on + strand, within fepDat 620.808 kb on + strand, within fepDat 620.892 kb on + strand, within fepDat 620.900 kb on - strand, within fepDat 620.964 kb on - strand, within fepDat 620.964 kb on - strand, within fepDat 620.970 kb on + strand, within fepDat 620.970 kb on + strand, within fepDat 621.018 kb on - strand, within fepDat 621.228 kb on - strand, within fepDat 621.295 kb on - strand, within fepDat 621.295 kb on - strand, within fepDat 621.331 kb on + strandat 621.474 kb on - strandat 621.474 kb on - strandat 621.487 kb on - strandat 621.608 kb on + strandat 621.654 kb on - strand, within ybdAat 621.727 kb on - strand, within ybdAat 621.819 kb on + strand, within ybdAat 621.846 kb on + strand, within ybdAat 621.846 kb on + strand, within ybdAat 621.966 kb on + strand, within ybdAat 622.236 kb on + strand, within ybdAat 622.246 kb on + strand, within ybdAat 622.255 kb on - strand, within ybdAat 622.255 kb on - strand, within ybdAat 622.365 kb on + strand, within ybdAat 622.365 kb on + strand, within ybdAat 622.409 kb on - strand, within ybdAat 622.434 kb on + strand, within ybdAat 622.441 kb on - strand, within ybdAat 622.441 kb on - strand, within ybdAat 622.472 kb on + strand, within ybdAat 622.472 kb on + strand, within ybdAat 622.491 kb on + strand, within ybdAat 622.596 kb on + strand, within ybdAat 622.596 kb on + strand, within ybdAat 622.611 kb on - strand, within ybdAat 622.611 kb on - strand, within ybdAat 622.615 kb on + strand, within ybdAat 622.615 kb on + strand, within ybdAat 622.801 kb on - strandat 622.819 kb on + strandat 622.819 kb on + strandat 622.847 kb on - strandat 622.875 kb on + strand, within fepBat 622.875 kb on + strand, within fepBat 622.899 kb on + strand, within fepBat 622.917 kb on + strand, within fepBat 622.923 kb on + strand, within fepBat 622.958 kb on + strand, within fepBat 623.019 kb on + strand, within fepBat 623.019 kb on + strand, within fepBat 623.028 kb on - strand, within fepBat 623.028 kb on - strand, within fepBat 623.032 kb on + strand, within fepBat 623.119 kb on + strand, within fepBat 623.140 kb on - strand, within fepBat 623.148 kb on - strand, within fepBat 623.148 kb on - strand, within fepBat 623.199 kb on - strand, within fepBat 623.253 kb on - strand, within fepBat 623.341 kb on - strand, within fepBat 623.405 kb on - strand, within fepBat 623.405 kb on - strand, within fepBat 623.426 kb on + strand, within fepBat 623.426 kb on + strand, within fepBat 623.458 kb on - strand, within fepBat 623.458 kb on - strand, within fepBat 623.467 kb on - strand, within fepBat 623.467 kb on - strand, within fepBat 623.470 kb on - strand, within fepBat 623.470 kb on - strand, within fepBat 623.563 kb on + strand, within fepBat 623.576 kb on - strand, within fepBat 623.576 kb on - strand, within fepBat 623.591 kb on - strand, within fepBat 623.591 kb on - strand, within fepBat 623.610 kb on + strand, within fepBat 623.672 kb on + strandat 623.708 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction GFP_control
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620,634 - fepD b0590 0.22 +1.0
620,653 - fepD b0590 0.24 -2.0
620,653 - fepD b0590 0.24 -2.9
620,664 - fepD b0590 0.25 +0.2
620,672 - fepD b0590 0.26 -0.0
620,728 - fepD b0590 0.32 +0.9
620,749 + fepD b0590 0.34 -3.4
620,749 + fepD b0590 0.34 -0.2
620,749 + fepD b0590 0.34 -0.8
620,780 - fepD b0590 0.37 -0.8
620,808 + fepD b0590 0.40 -1.1
620,808 + fepD b0590 0.40 -0.8
620,892 + fepD b0590 0.48 -1.3
620,900 - fepD b0590 0.49 -2.0
620,964 - fepD b0590 0.55 -0.3
620,964 - fepD b0590 0.55 -0.5
620,970 + fepD b0590 0.56 -0.5
620,970 + fepD b0590 0.56 +0.0
621,018 - fepD b0590 0.61 -1.5
621,228 - fepD b0590 0.82 +0.3
621,295 - fepD b0590 0.88 -0.8
621,295 - fepD b0590 0.88 -1.1
621,331 + +0.5
621,474 - +0.3
621,474 - -0.4
621,487 - +0.2
621,608 + -1.1
621,654 - ybdA b0591 0.10 -2.1
621,727 - ybdA b0591 0.16 -0.7
621,819 + ybdA b0591 0.24 -0.4
621,846 + ybdA b0591 0.26 +2.4
621,846 + ybdA b0591 0.26 -0.0
621,966 + ybdA b0591 0.35 -1.7
622,236 + ybdA b0591 0.57 -1.0
622,246 + ybdA b0591 0.58 -0.6
622,255 - ybdA b0591 0.59 +2.1
622,255 - ybdA b0591 0.59 +2.2
622,365 + ybdA b0591 0.67 +1.3
622,365 + ybdA b0591 0.67 +1.3
622,409 - ybdA b0591 0.71 +0.9
622,434 + ybdA b0591 0.73 +0.3
622,441 - ybdA b0591 0.73 +1.1
622,441 - ybdA b0591 0.73 +2.8
622,472 + ybdA b0591 0.76 -0.5
622,472 + ybdA b0591 0.76 -0.2
622,491 + ybdA b0591 0.77 +0.1
622,596 + ybdA b0591 0.86 -0.0
622,596 + ybdA b0591 0.86 +1.2
622,611 - ybdA b0591 0.87 +1.4
622,611 - ybdA b0591 0.87 +0.9
622,615 + ybdA b0591 0.87 +1.0
622,615 + ybdA b0591 0.87 +0.4
622,801 - +0.9
622,819 + -1.8
622,819 + +0.2
622,847 - +0.5
622,875 + fepB b0592 0.10 -0.2
622,875 + fepB b0592 0.10 +0.5
622,899 + fepB b0592 0.13 +0.9
622,917 + fepB b0592 0.15 -0.6
622,923 + fepB b0592 0.15 -3.1
622,958 + fepB b0592 0.19 +0.6
623,019 + fepB b0592 0.25 -1.9
623,019 + fepB b0592 0.25 -1.2
623,028 - fepB b0592 0.26 -0.7
623,028 - fepB b0592 0.26 -2.6
623,032 + fepB b0592 0.27 -0.5
623,119 + fepB b0592 0.36 +0.4
623,140 - fepB b0592 0.38 -0.1
623,148 - fepB b0592 0.39 -0.5
623,148 - fepB b0592 0.39 +1.4
623,199 - fepB b0592 0.44 -2.0
623,253 - fepB b0592 0.50 -1.6
623,341 - fepB b0592 0.59 +0.1
623,405 - fepB b0592 0.66 -2.2
623,405 - fepB b0592 0.66 +0.3
623,426 + fepB b0592 0.68 -0.9
623,426 + fepB b0592 0.68 -0.2
623,458 - fepB b0592 0.71 -0.1
623,458 - fepB b0592 0.71 -1.7
623,467 - fepB b0592 0.72 -0.1
623,467 - fepB b0592 0.72 -2.2
623,470 - fepB b0592 0.72 -3.1
623,470 - fepB b0592 0.72 -2.2
623,563 + fepB b0592 0.82 +0.7
623,576 - fepB b0592 0.83 -3.1
623,576 - fepB b0592 0.83 +0.7
623,591 - fepB b0592 0.85 -0.2
623,591 - fepB b0592 0.85 +1.5
623,610 + fepB b0592 0.87 +0.8
623,672 + -2.7
623,708 + +0.9

Or see this region's nucleotide sequence