Experiment: GFP_control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaB and wcaA are separated by 2 nucleotides wcaA and b2060 are separated by 92 nucleotides b2060 and wzb are separated by 2 nucleotides wzb and wza are separated by 5 nucleotides
b2058: wcaB - predicted acyl transferase (NCBI), at 2,130,091 to 2,130,579
wcaB
b2059: wcaA - predicted glycosyl transferase (NCBI), at 2,130,582 to 2,131,421
wcaA
b2060: b2060 - orf, hypothetical protein (VIMSS), at 2,131,514 to 2,133,676
b2060
b2061: wzb - protein-tyrosine phosphatase (NCBI), at 2,133,679 to 2,134,122
wzb
b2062: wza - lipoprotein required for capsular polysaccharide translocation through the outer membrane (NCBI), at 2,134,128 to 2,135,267
wza
Position (kb)
2131
2132
2133
2134 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2130.625 kb on + strand at 2130.691 kb on - strand, within wcaA at 2130.691 kb on - strand, within wcaA at 2130.692 kb on - strand, within wcaA at 2130.813 kb on + strand, within wcaA at 2130.841 kb on + strand, within wcaA at 2130.889 kb on - strand, within wcaA at 2131.196 kb on - strand, within wcaA at 2131.212 kb on + strand, within wcaA at 2131.306 kb on - strand, within wcaA at 2131.332 kb on - strand, within wcaA at 2131.395 kb on + strand at 2131.395 kb on + strand at 2131.421 kb on - strand at 2131.519 kb on - strand at 2131.589 kb on + strand at 2131.691 kb on - strand at 2131.691 kb on - strand at 2131.830 kb on - strand, within b2060 at 2131.840 kb on + strand, within b2060 at 2131.840 kb on + strand, within b2060 at 2131.903 kb on - strand, within b2060 at 2131.960 kb on - strand, within b2060 at 2132.123 kb on + strand, within b2060 at 2132.297 kb on + strand, within b2060 at 2132.405 kb on + strand, within b2060 at 2132.469 kb on + strand, within b2060 at 2132.535 kb on - strand, within b2060 at 2132.698 kb on + strand, within b2060 at 2132.698 kb on + strand, within b2060 at 2132.842 kb on - strand, within b2060 at 2132.973 kb on + strand, within b2060 at 2132.981 kb on - strand, within b2060 at 2133.037 kb on - strand, within b2060 at 2133.348 kb on - strand, within b2060 at 2133.494 kb on - strand at 2133.538 kb on - strand at 2133.685 kb on - strand at 2134.043 kb on + strand, within wzb at 2134.142 kb on - strand at 2134.142 kb on - strand at 2134.157 kb on - strand at 2134.208 kb on - strand at 2134.244 kb on - strand, within wza at 2134.244 kb on - strand, within wza at 2134.329 kb on + strand, within wza at 2134.385 kb on + strand, within wza at 2134.409 kb on + strand, within wza at 2134.417 kb on - strand, within wza at 2134.417 kb on - strand, within wza at 2134.427 kb on - strand, within wza at 2134.427 kb on - strand, within wza at 2134.545 kb on + strand, within wza at 2134.545 kb on + strand, within wza at 2134.545 kb on + strand, within wza at 2134.599 kb on + strand, within wza at 2134.604 kb on + strand, within wza
Per-strain Table
Position Strand Gene LocusTag Fraction GFP_control remove 2,130,625 + +0.7 2,130,691 - wcaA b2059 0.13 +0.1 2,130,691 - wcaA b2059 0.13 +0.3 2,130,692 - wcaA b2059 0.13 -0.4 2,130,813 + wcaA b2059 0.28 -2.1 2,130,841 + wcaA b2059 0.31 +0.6 2,130,889 - wcaA b2059 0.37 -1.0 2,131,196 - wcaA b2059 0.73 -1.4 2,131,212 + wcaA b2059 0.75 +0.7 2,131,306 - wcaA b2059 0.86 +0.7 2,131,332 - wcaA b2059 0.89 -1.1 2,131,395 + -0.3 2,131,395 + +0.4 2,131,421 - +0.5 2,131,519 - +0.8 2,131,589 + -1.8 2,131,691 - +0.2 2,131,691 - -1.2 2,131,830 - b2060 b2060 0.15 +0.1 2,131,840 + b2060 b2060 0.15 -0.9 2,131,840 + b2060 b2060 0.15 -1.8 2,131,903 - b2060 b2060 0.18 -0.7 2,131,960 - b2060 b2060 0.21 -1.3 2,132,123 + b2060 b2060 0.28 +0.2 2,132,297 + b2060 b2060 0.36 +0.2 2,132,405 + b2060 b2060 0.41 -1.1 2,132,469 + b2060 b2060 0.44 -0.9 2,132,535 - b2060 b2060 0.47 -2.0 2,132,698 + b2060 b2060 0.55 -3.2 2,132,698 + b2060 b2060 0.55 -1.7 2,132,842 - b2060 b2060 0.61 +0.1 2,132,973 + b2060 b2060 0.67 -1.9 2,132,981 - b2060 b2060 0.68 -4.2 2,133,037 - b2060 b2060 0.70 +0.0 2,133,348 - b2060 b2060 0.85 -0.3 2,133,494 - -0.2 2,133,538 - -1.0 2,133,685 - -0.7 2,134,043 + wzb b2061 0.82 +1.0 2,134,142 - -2.3 2,134,142 - -0.6 2,134,157 - -1.0 2,134,208 - -0.3 2,134,244 - wza b2062 0.10 +2.0 2,134,244 - wza b2062 0.10 -2.4 2,134,329 + wza b2062 0.18 +1.0 2,134,385 + wza b2062 0.23 -0.2 2,134,409 + wza b2062 0.25 +1.3 2,134,417 - wza b2062 0.25 +0.7 2,134,417 - wza b2062 0.25 +0.2 2,134,427 - wza b2062 0.26 -0.0 2,134,427 - wza b2062 0.26 -0.6 2,134,545 + wza b2062 0.37 -1.2 2,134,545 + wza b2062 0.37 +0.5 2,134,545 + wza b2062 0.37 +0.7 2,134,599 + wza b2062 0.41 +1.0 2,134,604 + wza b2062 0.42 +1.0
Or see this region's nucleotide sequence