Strain Fitness in Escherichia coli BW25113 around b4602

Experiment: Ying_Nucleobase14 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydhP and ynhF are separated by 165 nucleotidesynhF and purR are separated by 298 nucleotides b1657: ydhP - predicted transporter (NCBI), at 1,734,145 to 1,735,314 ydhP b4602: ynhF - hypothetical protein (RefSeq), at 1,735,480 to 1,735,569 ynhF b1658: purR - DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI), at 1,735,868 to 1,736,893 purR Position (kb) 1735 1736Strain fitness (log2 ratio) -2 -1 0 1at 1734.590 kb on - strand, within ydhPat 1734.609 kb on + strand, within ydhPat 1734.686 kb on + strand, within ydhPat 1734.726 kb on - strand, within ydhPat 1734.906 kb on - strand, within ydhPat 1734.939 kb on - strand, within ydhPat 1735.214 kb on - strandat 1735.316 kb on + strandat 1735.660 kb on - strandat 1735.660 kb on - strandat 1735.783 kb on + strandat 1735.783 kb on + strandat 1735.791 kb on - strandat 1736.132 kb on + strand, within purRat 1736.147 kb on - strand, within purRat 1736.235 kb on - strand, within purRat 1736.243 kb on - strand, within purRat 1736.255 kb on + strand, within purRat 1736.255 kb on + strand, within purRat 1736.297 kb on + strand, within purRat 1736.319 kb on + strand, within purRat 1736.347 kb on - strand, within purRat 1736.506 kb on + strand, within purRat 1736.506 kb on + strand, within purR

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 3
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1,734,590 - ydhP b1657 0.38 +0.3
1,734,609 + ydhP b1657 0.40 -0.3
1,734,686 + ydhP b1657 0.46 -0.5
1,734,726 - ydhP b1657 0.50 -0.1
1,734,906 - ydhP b1657 0.65 +0.3
1,734,939 - ydhP b1657 0.68 +0.6
1,735,214 - -0.1
1,735,316 + +0.2
1,735,660 - -0.9
1,735,660 - -0.4
1,735,783 + +1.1
1,735,783 + +0.2
1,735,791 - -1.2
1,736,132 + purR b1658 0.26 -2.3
1,736,147 - purR b1658 0.27 -2.3
1,736,235 - purR b1658 0.36 -1.4
1,736,243 - purR b1658 0.37 -1.0
1,736,255 + purR b1658 0.38 +0.4
1,736,255 + purR b1658 0.38 -0.3
1,736,297 + purR b1658 0.42 -1.2
1,736,319 + purR b1658 0.44 -1.4
1,736,347 - purR b1658 0.47 -1.6
1,736,506 + purR b1658 0.62 -0.2
1,736,506 + purR b1658 0.62 -1.5

Or see this region's nucleotide sequence