Experiment: Ying_Nucleobase14 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sbp and cdh are separated by 106 nucleotides cdh and tpiA are separated by 54 nucleotides tpiA and yiiQ are separated by 107 nucleotides yiiQ and yiiR are separated by 100 nucleotides
b3917: sbp - sulfate transporter subunit (NCBI), at 4,106,857 to 4,107,846
sbp
b3918: cdh - CDP-diacylglycerol pyrophosphatase (NCBI), at 4,107,953 to 4,108,708
cdh
b3919: tpiA - triosephosphate isomerase (NCBI), at 4,108,763 to 4,109,530
tpiA
b3920: yiiQ - hypothetical protein (NCBI), at 4,109,638 to 4,110,237
yiiQ
b3921: yiiR - conserved inner membrane protein (NCBI), at 4,110,338 to 4,110,778
yiiR
Position (kb)
4108
4109
4110 Strain fitness (log2 ratio)
-1
0
1 at 4107.771 kb on + strand at 4107.831 kb on + strand at 4107.831 kb on + strand at 4107.840 kb on + strand at 4107.929 kb on + strand at 4108.052 kb on + strand, within cdh at 4108.052 kb on + strand, within cdh at 4108.058 kb on - strand, within cdh at 4108.168 kb on - strand, within cdh at 4108.170 kb on - strand, within cdh at 4108.170 kb on - strand, within cdh at 4108.172 kb on + strand, within cdh at 4108.183 kb on + strand, within cdh at 4108.200 kb on + strand, within cdh at 4108.200 kb on + strand, within cdh at 4108.202 kb on - strand, within cdh at 4108.251 kb on + strand, within cdh at 4108.307 kb on + strand, within cdh at 4108.307 kb on + strand, within cdh at 4108.342 kb on - strand, within cdh at 4108.386 kb on - strand, within cdh at 4108.391 kb on - strand, within cdh at 4108.417 kb on - strand, within cdh at 4108.417 kb on - strand, within cdh at 4108.532 kb on - strand, within cdh at 4108.568 kb on - strand, within cdh at 4108.568 kb on - strand, within cdh at 4108.576 kb on - strand, within cdh at 4108.579 kb on + strand, within cdh at 4108.579 kb on + strand, within cdh at 4108.601 kb on - strand, within cdh at 4108.651 kb on + strand at 4109.521 kb on - strand at 4109.552 kb on - strand at 4109.575 kb on - strand at 4109.575 kb on - strand at 4109.671 kb on + strand at 4109.675 kb on + strand at 4109.675 kb on + strand at 4109.727 kb on + strand, within yiiQ at 4109.739 kb on + strand, within yiiQ at 4109.752 kb on - strand, within yiiQ at 4109.759 kb on + strand, within yiiQ at 4109.838 kb on + strand, within yiiQ at 4109.838 kb on + strand, within yiiQ at 4109.838 kb on + strand, within yiiQ at 4109.846 kb on - strand, within yiiQ at 4109.866 kb on - strand, within yiiQ at 4109.963 kb on + strand, within yiiQ at 4109.963 kb on + strand, within yiiQ at 4110.004 kb on + strand, within yiiQ at 4110.007 kb on - strand, within yiiQ at 4110.007 kb on - strand, within yiiQ at 4110.106 kb on - strand, within yiiQ at 4110.116 kb on - strand, within yiiQ at 4110.135 kb on - strand, within yiiQ at 4110.135 kb on - strand, within yiiQ at 4110.159 kb on - strand, within yiiQ at 4110.177 kb on + strand, within yiiQ at 4110.177 kb on + strand, within yiiQ at 4110.185 kb on - strand at 4110.198 kb on - strand at 4110.232 kb on - strand at 4110.233 kb on + strand at 4110.342 kb on + strand at 4110.344 kb on + strand at 4110.375 kb on + strand at 4110.402 kb on + strand, within yiiR at 4110.407 kb on + strand, within yiiR at 4110.407 kb on + strand, within yiiR at 4110.445 kb on + strand, within yiiR at 4110.449 kb on + strand, within yiiR at 4110.465 kb on + strand, within yiiR at 4110.502 kb on - strand, within yiiR
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 3 remove 4,107,771 + +0.8 4,107,831 + -0.6 4,107,831 + +0.2 4,107,840 + -0.9 4,107,929 + -0.2 4,108,052 + cdh b3918 0.13 +0.2 4,108,052 + cdh b3918 0.13 +0.3 4,108,058 - cdh b3918 0.14 +0.9 4,108,168 - cdh b3918 0.28 -1.3 4,108,170 - cdh b3918 0.29 +0.1 4,108,170 - cdh b3918 0.29 +0.2 4,108,172 + cdh b3918 0.29 -0.1 4,108,183 + cdh b3918 0.30 -0.4 4,108,200 + cdh b3918 0.33 +0.7 4,108,200 + cdh b3918 0.33 -0.5 4,108,202 - cdh b3918 0.33 -0.0 4,108,251 + cdh b3918 0.39 -0.3 4,108,307 + cdh b3918 0.47 -0.1 4,108,307 + cdh b3918 0.47 -0.1 4,108,342 - cdh b3918 0.51 +0.1 4,108,386 - cdh b3918 0.57 +0.4 4,108,391 - cdh b3918 0.58 -0.3 4,108,417 - cdh b3918 0.61 +0.6 4,108,417 - cdh b3918 0.61 -0.1 4,108,532 - cdh b3918 0.77 -0.2 4,108,568 - cdh b3918 0.81 -0.2 4,108,568 - cdh b3918 0.81 +0.4 4,108,576 - cdh b3918 0.82 -0.5 4,108,579 + cdh b3918 0.83 -0.3 4,108,579 + cdh b3918 0.83 +0.0 4,108,601 - cdh b3918 0.86 -0.1 4,108,651 + -0.1 4,109,521 - -0.9 4,109,552 - +0.0 4,109,575 - +0.1 4,109,575 - +1.3 4,109,671 + +0.5 4,109,675 + +0.5 4,109,675 + +0.1 4,109,727 + yiiQ b3920 0.15 +0.0 4,109,739 + yiiQ b3920 0.17 -0.3 4,109,752 - yiiQ b3920 0.19 -0.4 4,109,759 + yiiQ b3920 0.20 +0.0 4,109,838 + yiiQ b3920 0.33 +0.4 4,109,838 + yiiQ b3920 0.33 -0.3 4,109,838 + yiiQ b3920 0.33 +0.1 4,109,846 - yiiQ b3920 0.35 +0.2 4,109,866 - yiiQ b3920 0.38 -1.1 4,109,963 + yiiQ b3920 0.54 +0.3 4,109,963 + yiiQ b3920 0.54 +0.9 4,110,004 + yiiQ b3920 0.61 +0.4 4,110,007 - yiiQ b3920 0.61 -0.3 4,110,007 - yiiQ b3920 0.61 -0.0 4,110,106 - yiiQ b3920 0.78 -0.1 4,110,116 - yiiQ b3920 0.80 +0.4 4,110,135 - yiiQ b3920 0.83 -0.2 4,110,135 - yiiQ b3920 0.83 +0.0 4,110,159 - yiiQ b3920 0.87 -0.3 4,110,177 + yiiQ b3920 0.90 +1.3 4,110,177 + yiiQ b3920 0.90 +0.0 4,110,185 - +0.3 4,110,198 - +0.4 4,110,232 - +1.2 4,110,233 + +0.3 4,110,342 + +0.1 4,110,344 + +0.4 4,110,375 + +0.6 4,110,402 + yiiR b3921 0.15 -1.0 4,110,407 + yiiR b3921 0.16 +0.6 4,110,407 + yiiR b3921 0.16 -0.1 4,110,445 + yiiR b3921 0.24 +0.0 4,110,449 + yiiR b3921 0.25 -0.2 4,110,465 + yiiR b3921 0.29 -0.4 4,110,502 - yiiR b3921 0.37 +1.3
Or see this region's nucleotide sequence