Strain Fitness in Escherichia coli BW25113 around b3288

Experiment: Ying_Nucleobase14 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsmf and def are separated by 129 nucleotidesdef and fmt are separated by 14 nucleotidesfmt and rsmB are separated by 45 nucleotides b4473: smf - hypothetical protein (NCBI), at 3,430,458 to 3,431,582 smf b3287: def - peptide deformylase (NCBI), at 3,431,712 to 3,432,221 def b3288: fmt - methionyl-tRNA formyltransferase (NCBI), at 3,432,236 to 3,433,183 fmt b3289: rsmB - 16S rRNA m5C967 methyltransferase, S-adenosyl-L-methionine-dependent (NCBI), at 3,433,229 to 3,434,518 rsmB Position (kb) 3432 3433 3434Strain fitness (log2 ratio) -2 -1 0 1at 3431.373 kb on + strand, within smfat 3431.376 kb on - strand, within smfat 3431.415 kb on - strand, within smfat 3431.415 kb on - strand, within smfat 3431.448 kb on + strand, within smfat 3431.448 kb on + strand, within smfat 3431.455 kb on - strand, within smfat 3431.455 kb on - strand, within smfat 3431.468 kb on + strand, within smfat 3431.474 kb on - strandat 3431.482 kb on + strandat 3431.509 kb on + strandat 3431.512 kb on + strandat 3431.517 kb on + strandat 3431.527 kb on - strandat 3431.578 kb on + strandat 3431.666 kb on - strandat 3431.697 kb on + strandat 3431.697 kb on + strandat 3432.172 kb on + strandat 3432.204 kb on + strandat 3432.204 kb on + strandat 3432.238 kb on + strandat 3433.170 kb on + strandat 3433.177 kb on + strandat 3433.178 kb on + strandat 3433.186 kb on - strandat 3433.186 kb on - strandat 3433.193 kb on + strandat 3433.193 kb on + strandat 3433.218 kb on + strandat 3433.260 kb on - strandat 3433.293 kb on + strandat 3433.374 kb on + strand, within rsmBat 3433.374 kb on + strand, within rsmBat 3433.374 kb on + strand, within rsmBat 3433.379 kb on - strand, within rsmBat 3433.384 kb on - strand, within rsmBat 3433.392 kb on - strand, within rsmBat 3433.393 kb on + strand, within rsmBat 3433.397 kb on + strand, within rsmBat 3433.397 kb on + strand, within rsmBat 3433.397 kb on + strand, within rsmBat 3433.397 kb on + strand, within rsmBat 3433.397 kb on + strand, within rsmBat 3433.397 kb on + strand, within rsmBat 3433.421 kb on - strand, within rsmBat 3433.421 kb on - strand, within rsmBat 3433.440 kb on - strand, within rsmBat 3433.447 kb on + strand, within rsmBat 3433.464 kb on + strand, within rsmBat 3433.466 kb on - strand, within rsmBat 3433.472 kb on - strand, within rsmBat 3433.497 kb on + strand, within rsmBat 3433.504 kb on + strand, within rsmBat 3433.511 kb on - strand, within rsmBat 3433.518 kb on - strand, within rsmBat 3433.523 kb on - strand, within rsmBat 3433.523 kb on - strand, within rsmBat 3433.525 kb on + strand, within rsmBat 3433.525 kb on + strand, within rsmBat 3433.567 kb on + strand, within rsmBat 3433.589 kb on + strand, within rsmBat 3433.629 kb on - strand, within rsmBat 3433.665 kb on + strand, within rsmBat 3433.665 kb on + strand, within rsmBat 3433.665 kb on + strand, within rsmBat 3433.671 kb on - strand, within rsmBat 3433.671 kb on - strand, within rsmBat 3433.673 kb on - strand, within rsmBat 3433.698 kb on + strand, within rsmBat 3433.706 kb on - strand, within rsmBat 3433.706 kb on - strand, within rsmBat 3433.706 kb on - strand, within rsmBat 3433.758 kb on + strand, within rsmBat 3433.837 kb on + strand, within rsmBat 3433.843 kb on + strand, within rsmBat 3433.843 kb on + strand, within rsmBat 3433.845 kb on - strand, within rsmBat 3433.845 kb on - strand, within rsmBat 3433.851 kb on + strand, within rsmBat 3433.859 kb on - strand, within rsmBat 3433.864 kb on - strand, within rsmBat 3433.864 kb on - strand, within rsmBat 3433.864 kb on - strand, within rsmBat 3433.877 kb on + strand, within rsmBat 3433.936 kb on + strand, within rsmBat 3433.939 kb on - strand, within rsmBat 3433.944 kb on - strand, within rsmBat 3433.953 kb on - strand, within rsmBat 3434.019 kb on + strand, within rsmBat 3434.027 kb on - strand, within rsmBat 3434.036 kb on + strand, within rsmBat 3434.071 kb on - strand, within rsmBat 3434.071 kb on - strand, within rsmBat 3434.112 kb on - strand, within rsmBat 3434.134 kb on - strandat 3434.141 kb on - strand, within rsmBat 3434.141 kb on - strand, within rsmBat 3434.147 kb on - strand, within rsmBat 3434.147 kb on - strand, within rsmBat 3434.150 kb on + strand, within rsmBat 3434.158 kb on - strand, within rsmB

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 3
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3,431,373 + smf b4473 0.81 -0.6
3,431,376 - smf b4473 0.82 +0.2
3,431,415 - smf b4473 0.85 -0.6
3,431,415 - smf b4473 0.85 +0.1
3,431,448 + smf b4473 0.88 +0.3
3,431,448 + smf b4473 0.88 -0.1
3,431,455 - smf b4473 0.89 -0.1
3,431,455 - smf b4473 0.89 -0.9
3,431,468 + smf b4473 0.90 -0.1
3,431,474 - +0.3
3,431,482 + -0.2
3,431,509 + -0.4
3,431,512 + +0.3
3,431,517 + +0.5
3,431,527 - +0.1
3,431,578 + +0.2
3,431,666 - +0.1
3,431,697 + +0.3
3,431,697 + +0.1
3,432,172 + -2.6
3,432,204 + -1.5
3,432,204 + -1.3
3,432,238 + +0.1
3,433,170 + -0.6
3,433,177 + +0.5
3,433,178 + -0.0
3,433,186 - +0.6
3,433,186 - -0.9
3,433,193 + +0.4
3,433,193 + -0.7
3,433,218 + -0.4
3,433,260 - -0.5
3,433,293 + +0.9
3,433,374 + rsmB b3289 0.11 +0.8
3,433,374 + rsmB b3289 0.11 -0.6
3,433,374 + rsmB b3289 0.11 +0.3
3,433,379 - rsmB b3289 0.12 -0.5
3,433,384 - rsmB b3289 0.12 -0.7
3,433,392 - rsmB b3289 0.13 +0.8
3,433,393 + rsmB b3289 0.13 +0.6
3,433,397 + rsmB b3289 0.13 +0.3
3,433,397 + rsmB b3289 0.13 +0.4
3,433,397 + rsmB b3289 0.13 -0.2
3,433,397 + rsmB b3289 0.13 -0.6
3,433,397 + rsmB b3289 0.13 +0.4
3,433,397 + rsmB b3289 0.13 -0.9
3,433,421 - rsmB b3289 0.15 -1.9
3,433,421 - rsmB b3289 0.15 +1.0
3,433,440 - rsmB b3289 0.16 -0.7
3,433,447 + rsmB b3289 0.17 +1.2
3,433,464 + rsmB b3289 0.18 +0.4
3,433,466 - rsmB b3289 0.18 -0.6
3,433,472 - rsmB b3289 0.19 -0.3
3,433,497 + rsmB b3289 0.21 +0.1
3,433,504 + rsmB b3289 0.21 +0.3
3,433,511 - rsmB b3289 0.22 -0.4
3,433,518 - rsmB b3289 0.22 +0.4
3,433,523 - rsmB b3289 0.23 +1.1
3,433,523 - rsmB b3289 0.23 +0.8
3,433,525 + rsmB b3289 0.23 +0.4
3,433,525 + rsmB b3289 0.23 +0.9
3,433,567 + rsmB b3289 0.26 +0.4
3,433,589 + rsmB b3289 0.28 +0.5
3,433,629 - rsmB b3289 0.31 -0.2
3,433,665 + rsmB b3289 0.34 +0.4
3,433,665 + rsmB b3289 0.34 +1.3
3,433,665 + rsmB b3289 0.34 -0.2
3,433,671 - rsmB b3289 0.34 -1.0
3,433,671 - rsmB b3289 0.34 -1.3
3,433,673 - rsmB b3289 0.34 -0.0
3,433,698 + rsmB b3289 0.36 +0.9
3,433,706 - rsmB b3289 0.37 -0.7
3,433,706 - rsmB b3289 0.37 +0.7
3,433,706 - rsmB b3289 0.37 +0.4
3,433,758 + rsmB b3289 0.41 +0.2
3,433,837 + rsmB b3289 0.47 +0.2
3,433,843 + rsmB b3289 0.48 -0.2
3,433,843 + rsmB b3289 0.48 +0.8
3,433,845 - rsmB b3289 0.48 -0.6
3,433,845 - rsmB b3289 0.48 -0.6
3,433,851 + rsmB b3289 0.48 +0.4
3,433,859 - rsmB b3289 0.49 -0.2
3,433,864 - rsmB b3289 0.49 -0.5
3,433,864 - rsmB b3289 0.49 -0.7
3,433,864 - rsmB b3289 0.49 -0.1
3,433,877 + rsmB b3289 0.50 +0.6
3,433,936 + rsmB b3289 0.55 -0.5
3,433,939 - rsmB b3289 0.55 -0.9
3,433,944 - rsmB b3289 0.55 +0.2
3,433,953 - rsmB b3289 0.56 +0.6
3,434,019 + rsmB b3289 0.61 -0.0
3,434,027 - rsmB b3289 0.62 +1.2
3,434,036 + rsmB b3289 0.63 +0.5
3,434,071 - rsmB b3289 0.65 -0.4
3,434,071 - rsmB b3289 0.65 +0.2
3,434,112 - rsmB b3289 0.68 +0.1
3,434,134 - -0.4
3,434,141 - rsmB b3289 0.71 -0.6
3,434,141 - rsmB b3289 0.71 +0.4
3,434,147 - rsmB b3289 0.71 -0.0
3,434,147 - rsmB b3289 0.71 -0.6
3,434,150 + rsmB b3289 0.71 +0.1
3,434,158 - rsmB b3289 0.72 -0.7

Or see this region's nucleotide sequence