Experiment: Ying_Nucleobase14 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppdB and ppdA overlap by 10 nucleotides ppdA and thyA are separated by 183 nucleotides thyA and lgt are separated by 6 nucleotides
b2825: ppdB - hypothetical protein (NCBI), at 2,961,175 to 2,961,738
ppdB
b2826: ppdA - hypothetical protein (NCBI), at 2,961,729 to 2,962,199
ppdA
b2827: thyA - thymidylate synthase (NCBI), at 2,962,383 to 2,963,177
thyA
b2828: lgt - prolipoprotein diacylglyceryl transferase (NCBI), at 2,963,184 to 2,964,059
lgt
Position (kb)
2962
2963
2964 Strain fitness (log2 ratio)
-2
-1
0
1 at 2961.469 kb on + strand, within ppdB at 2961.469 kb on + strand, within ppdB at 2961.483 kb on - strand, within ppdB at 2961.483 kb on - strand, within ppdB at 2961.493 kb on - strand, within ppdB at 2961.493 kb on - strand, within ppdB at 2961.518 kb on + strand, within ppdB at 2961.529 kb on - strand, within ppdB at 2961.529 kb on - strand, within ppdB at 2961.543 kb on - strand, within ppdB at 2961.543 kb on - strand, within ppdB at 2961.543 kb on - strand, within ppdB at 2961.664 kb on + strand, within ppdB at 2961.664 kb on + strand, within ppdB at 2961.794 kb on + strand, within ppdA at 2961.794 kb on + strand, within ppdA at 2961.832 kb on - strand, within ppdA at 2961.832 kb on - strand, within ppdA at 2961.852 kb on - strand, within ppdA at 2961.875 kb on + strand, within ppdA at 2961.875 kb on + strand, within ppdA at 2961.906 kb on + strand, within ppdA at 2961.918 kb on + strand, within ppdA at 2961.943 kb on + strand, within ppdA at 2961.943 kb on + strand, within ppdA at 2961.958 kb on + strand, within ppdA at 2961.958 kb on + strand, within ppdA at 2961.963 kb on + strand, within ppdA at 2962.113 kb on + strand, within ppdA at 2962.122 kb on - strand, within ppdA at 2962.130 kb on + strand, within ppdA at 2962.130 kb on + strand, within ppdA at 2962.149 kb on - strand, within ppdA at 2962.149 kb on - strand, within ppdA at 2962.151 kb on + strand, within ppdA at 2962.173 kb on - strand at 2962.175 kb on + strand at 2962.175 kb on + strand at 2962.175 kb on + strand at 2962.175 kb on + strand at 2962.183 kb on - strand at 2962.215 kb on - strand at 2962.271 kb on + strand at 2962.281 kb on + strand at 2962.281 kb on + strand at 2962.289 kb on - strand at 2962.289 kb on - strand at 2963.201 kb on - strand at 2963.239 kb on - strand at 2964.140 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 3 remove 2,961,469 + ppdB b2825 0.52 +1.4 2,961,469 + ppdB b2825 0.52 +0.6 2,961,483 - ppdB b2825 0.55 +1.3 2,961,483 - ppdB b2825 0.55 -0.2 2,961,493 - ppdB b2825 0.56 -0.6 2,961,493 - ppdB b2825 0.56 -0.5 2,961,518 + ppdB b2825 0.61 -0.6 2,961,529 - ppdB b2825 0.63 -0.1 2,961,529 - ppdB b2825 0.63 -0.0 2,961,543 - ppdB b2825 0.65 -0.5 2,961,543 - ppdB b2825 0.65 -0.3 2,961,543 - ppdB b2825 0.65 -0.0 2,961,664 + ppdB b2825 0.87 -2.1 2,961,664 + ppdB b2825 0.87 -0.3 2,961,794 + ppdA b2826 0.14 +0.3 2,961,794 + ppdA b2826 0.14 -0.3 2,961,832 - ppdA b2826 0.22 -0.4 2,961,832 - ppdA b2826 0.22 -0.3 2,961,852 - ppdA b2826 0.26 -0.3 2,961,875 + ppdA b2826 0.31 +1.0 2,961,875 + ppdA b2826 0.31 -1.1 2,961,906 + ppdA b2826 0.38 -0.3 2,961,918 + ppdA b2826 0.40 -0.1 2,961,943 + ppdA b2826 0.45 -0.1 2,961,943 + ppdA b2826 0.45 +0.0 2,961,958 + ppdA b2826 0.49 +0.4 2,961,958 + ppdA b2826 0.49 -0.5 2,961,963 + ppdA b2826 0.50 +0.3 2,962,113 + ppdA b2826 0.82 -0.4 2,962,122 - ppdA b2826 0.83 +0.4 2,962,130 + ppdA b2826 0.85 +0.1 2,962,130 + ppdA b2826 0.85 -0.2 2,962,149 - ppdA b2826 0.89 -0.3 2,962,149 - ppdA b2826 0.89 -0.3 2,962,151 + ppdA b2826 0.90 -0.2 2,962,173 - -0.3 2,962,175 + -0.1 2,962,175 + -0.2 2,962,175 + -0.5 2,962,175 + +0.3 2,962,183 - +0.2 2,962,215 - -0.2 2,962,271 + -0.2 2,962,281 + +0.2 2,962,281 + +0.2 2,962,289 - -0.3 2,962,289 - -0.2 2,963,201 - -0.3 2,963,239 - -0.2 2,964,140 - +1.1
Or see this region's nucleotide sequence