Experiment: Ying_Nucleobase14 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt eco and mqo are separated by 714 nucleotides mqo and yojI are separated by 217 nucleotides
b2209: eco - ecotin precursor (NCBI), at 2,301,927 to 2,302,415
eco
b2210: mqo - malate:quinone oxidoreductase (NCBI), at 2,303,130 to 2,304,776
mqo
b2211: yojI - fused predicted multidrug transport subunits of ABC superfamily: membrane component/ATP-binding component (NCBI), at 2,304,994 to 2,306,637
yojI
Position (kb)
2303
2304
2305 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6 at 2302.294 kb on + strand, within eco at 2302.310 kb on - strand, within eco at 2302.522 kb on + strand at 2302.748 kb on + strand at 2302.763 kb on + strand at 2302.763 kb on + strand at 2302.773 kb on + strand at 2303.223 kb on - strand at 2303.225 kb on + strand at 2303.226 kb on + strand at 2303.244 kb on + strand at 2303.346 kb on - strand, within mqo at 2303.346 kb on - strand, within mqo at 2303.421 kb on - strand, within mqo at 2303.421 kb on - strand, within mqo at 2303.579 kb on - strand, within mqo at 2303.672 kb on + strand, within mqo at 2303.680 kb on - strand, within mqo at 2303.694 kb on + strand, within mqo at 2303.694 kb on + strand, within mqo at 2303.702 kb on - strand, within mqo at 2303.702 kb on - strand, within mqo at 2303.702 kb on - strand, within mqo at 2303.767 kb on + strand, within mqo at 2303.843 kb on - strand, within mqo at 2303.919 kb on + strand at 2303.955 kb on + strand, within mqo at 2304.051 kb on - strand, within mqo at 2304.051 kb on - strand, within mqo at 2304.174 kb on - strand, within mqo at 2304.174 kb on - strand, within mqo at 2304.237 kb on + strand at 2304.388 kb on + strand, within mqo at 2304.409 kb on + strand, within mqo at 2304.417 kb on - strand, within mqo at 2304.444 kb on - strand, within mqo at 2304.524 kb on + strand, within mqo at 2304.577 kb on + strand, within mqo at 2304.578 kb on + strand, within mqo at 2304.578 kb on + strand, within mqo at 2304.991 kb on + strand at 2305.038 kb on + strand at 2305.038 kb on + strand at 2305.045 kb on + strand at 2305.067 kb on - strand at 2305.102 kb on + strand at 2305.120 kb on - strand at 2305.289 kb on + strand, within yojI at 2305.302 kb on + strand, within yojI at 2305.390 kb on - strand, within yojI at 2305.439 kb on - strand, within yojI at 2305.529 kb on + strand, within yojI at 2305.616 kb on - strand, within yojI at 2305.720 kb on - strand, within yojI at 2305.720 kb on - strand, within yojI at 2305.747 kb on + strand, within yojI at 2305.747 kb on + strand, within yojI at 2305.760 kb on + strand, within yojI at 2305.763 kb on + strand, within yojI
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 3 remove 2,302,294 + eco b2209 0.75 -0.5 2,302,310 - eco b2209 0.78 -0.4 2,302,522 + -0.3 2,302,748 + -0.3 2,302,763 + -0.8 2,302,763 + +0.3 2,302,773 + +0.0 2,303,223 - +0.4 2,303,225 + -0.3 2,303,226 + -0.1 2,303,244 + -0.9 2,303,346 - mqo b2210 0.13 +0.4 2,303,346 - mqo b2210 0.13 +0.2 2,303,421 - mqo b2210 0.18 +0.7 2,303,421 - mqo b2210 0.18 +0.1 2,303,579 - mqo b2210 0.27 +0.1 2,303,672 + mqo b2210 0.33 +0.0 2,303,680 - mqo b2210 0.33 +0.0 2,303,694 + mqo b2210 0.34 +0.1 2,303,694 + mqo b2210 0.34 +0.4 2,303,702 - mqo b2210 0.35 -0.2 2,303,702 - mqo b2210 0.35 -0.3 2,303,702 - mqo b2210 0.35 +0.3 2,303,767 + mqo b2210 0.39 -0.1 2,303,843 - mqo b2210 0.43 +0.4 2,303,919 + +0.7 2,303,955 + mqo b2210 0.50 +0.1 2,304,051 - mqo b2210 0.56 +0.1 2,304,051 - mqo b2210 0.56 -0.2 2,304,174 - mqo b2210 0.63 -0.0 2,304,174 - mqo b2210 0.63 -0.0 2,304,237 + -0.4 2,304,388 + mqo b2210 0.76 +0.3 2,304,409 + mqo b2210 0.78 +0.1 2,304,417 - mqo b2210 0.78 +0.2 2,304,444 - mqo b2210 0.80 -0.3 2,304,524 + mqo b2210 0.85 +0.0 2,304,577 + mqo b2210 0.88 +0.0 2,304,578 + mqo b2210 0.88 +0.8 2,304,578 + mqo b2210 0.88 +0.2 2,304,991 + +0.3 2,305,038 + +0.8 2,305,038 + -1.3 2,305,045 + +0.3 2,305,067 - -0.1 2,305,102 + -0.0 2,305,120 - +0.4 2,305,289 + yojI b2211 0.18 +0.0 2,305,302 + yojI b2211 0.19 -0.4 2,305,390 - yojI b2211 0.24 +6.7 2,305,439 - yojI b2211 0.27 -0.5 2,305,529 + yojI b2211 0.33 -0.0 2,305,616 - yojI b2211 0.38 +0.6 2,305,720 - yojI b2211 0.44 +0.3 2,305,720 - yojI b2211 0.44 -0.1 2,305,747 + yojI b2211 0.46 -0.7 2,305,747 + yojI b2211 0.46 +0.1 2,305,760 + yojI b2211 0.47 +0.7 2,305,763 + yojI b2211 0.47 +0.1
Or see this region's nucleotide sequence