Experiment: Ying_Nucleobase14 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt galM and galK overlap by 7 nucleotides galK and galT are separated by 3 nucleotides
b0756: galM - galactose-1-epimerase (mutarotase) (NCBI), at 787,020 to 788,060
galM
b0757: galK - galactokinase GalK (EC 2.7.1.6) <small>(from data)</small>, at 788,054 to 789,202
galK
b0758: galT - galactose-1-phosphate uridylyltransferase (NCBI), at 789,206 to 790,252
galT
Position (kb) 788
789
790 Strain fitness (log2 ratio) -2
-1
0
1
2 at 787.080 kb on - strand at 787.087 kb on + strand at 787.091 kb on - strand at 787.106 kb on + strand at 787.172 kb on - strand, within galM at 787.307 kb on - strand, within galM at 787.308 kb on - strand, within galM at 787.414 kb on - strand, within galM at 787.510 kb on - strand, within galM at 787.569 kb on - strand, within galM at 787.749 kb on + strand, within galM at 787.826 kb on + strand, within galM at 787.876 kb on - strand, within galM at 787.962 kb on - strand at 787.962 kb on - strand at 787.985 kb on + strand at 788.062 kb on + strand at 788.062 kb on + strand at 788.065 kb on - strand at 788.252 kb on + strand, within galK at 788.406 kb on - strand, within galK at 788.406 kb on - strand, within galK at 788.436 kb on - strand, within galK at 788.479 kb on + strand, within galK at 788.479 kb on + strand, within galK at 788.487 kb on - strand, within galK at 788.487 kb on - strand, within galK at 788.518 kb on - strand, within galK at 788.531 kb on + strand, within galK at 788.531 kb on + strand, within galK at 788.531 kb on + strand, within galK at 788.531 kb on + strand, within galK at 788.600 kb on - strand, within galK at 788.610 kb on + strand, within galK at 788.644 kb on - strand, within galK at 788.648 kb on - strand, within galK at 788.732 kb on + strand, within galK at 788.829 kb on + strand, within galK at 788.832 kb on - strand, within galK at 789.031 kb on + strand, within galK at 789.039 kb on - strand, within galK at 789.050 kb on + strand, within galK at 789.063 kb on + strand, within galK at 789.063 kb on + strand, within galK at 789.241 kb on - strand at 789.338 kb on - strand, within galT at 789.338 kb on - strand, within galT at 789.425 kb on - strand, within galT at 789.425 kb on - strand at 789.433 kb on - strand, within galT at 789.581 kb on - strand, within galT at 789.661 kb on + strand, within galT at 789.712 kb on - strand, within galT at 789.712 kb on - strand, within galT at 789.774 kb on - strand, within galT at 789.811 kb on + strand, within galT at 789.908 kb on + strand, within galT at 790.161 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 3 remove 787,080 - +0.8 787,087 + -0.8 787,091 - -0.8 787,106 + -0.6 787,172 - galM b0756 0.15 -0.1 787,307 - galM b0756 0.28 +0.5 787,308 - galM b0756 0.28 -0.5 787,414 - galM b0756 0.38 -0.4 787,510 - galM b0756 0.47 -0.6 787,569 - galM b0756 0.53 -0.5 787,749 + galM b0756 0.70 -0.2 787,826 + galM b0756 0.77 +0.1 787,876 - galM b0756 0.82 +0.0 787,962 - +0.1 787,962 - +0.3 787,985 + +0.2 788,062 + -0.6 788,062 + +1.0 788,065 - +0.7 788,252 + galK b0757 0.17 -0.4 788,406 - galK b0757 0.31 -2.3 788,406 - galK b0757 0.31 -0.3 788,436 - galK b0757 0.33 -0.5 788,479 + galK b0757 0.37 +0.0 788,479 + galK b0757 0.37 -0.3 788,487 - galK b0757 0.38 +0.1 788,487 - galK b0757 0.38 -0.2 788,518 - galK b0757 0.40 +0.1 788,531 + galK b0757 0.42 -0.5 788,531 + galK b0757 0.42 +0.1 788,531 + galK b0757 0.42 -1.6 788,531 + galK b0757 0.42 -0.1 788,600 - galK b0757 0.48 +0.1 788,610 + galK b0757 0.48 +0.5 788,644 - galK b0757 0.51 -0.9 788,648 - galK b0757 0.52 -0.6 788,732 + galK b0757 0.59 -0.4 788,829 + galK b0757 0.67 -0.7 788,832 - galK b0757 0.68 +0.7 789,031 + galK b0757 0.85 -0.3 789,039 - galK b0757 0.86 -0.2 789,050 + galK b0757 0.87 -0.4 789,063 + galK b0757 0.88 -2.0 789,063 + galK b0757 0.88 +2.2 789,241 - +0.1 789,338 - galT b0758 0.13 +0.8 789,338 - galT b0758 0.13 -0.9 789,425 - galT b0758 0.21 -0.0 789,425 - -0.1 789,433 - galT b0758 0.22 +0.0 789,581 - galT b0758 0.36 -0.3 789,661 + galT b0758 0.43 -0.7 789,712 - galT b0758 0.48 +0.0 789,712 - galT b0758 0.48 +0.5 789,774 - galT b0758 0.54 -0.2 789,811 + galT b0758 0.58 -0.2 789,908 + galT b0758 0.67 -1.0 790,161 - -0.6
Or see this region's nucleotide sequence