Experiment: Ying_Nucleobase14 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sucC and sucD overlap by 1 nucleotides sucD and mngR are separated by 103 nucleotides mngR and mngA are separated by 108 nucleotides
b0728: sucC - succinyl-CoA synthetase subunit beta (NCBI), at 762,237 to 763,403
sucC
b0729: sucD - succinyl-CoA synthetase subunit alpha (NCBI), at 763,403 to 764,272
sucD
b0730: mngR - DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI), at 764,376 to 765,098
mngR
b0731: mngA - fused 2-O-a-mannosyl-D-glycerate specific PTS enzymes: IIA component/IIB component/IIC component (NCBI), at 765,207 to 767,183
mngA
Position (kb) 764
765
766 Strain fitness (log2 ratio) -2
-1
0
1
2 at 763.625 kb on + strand, within sucD at 763.625 kb on + strand, within sucD at 763.712 kb on + strand, within sucD at 763.712 kb on + strand, within sucD at 763.773 kb on + strand, within sucD at 763.871 kb on - strand, within sucD at 763.901 kb on + strand, within sucD at 764.252 kb on - strand at 764.279 kb on + strand at 764.302 kb on + strand at 764.302 kb on + strand at 764.311 kb on + strand at 764.319 kb on + strand at 764.349 kb on + strand at 764.480 kb on + strand, within mngR at 764.480 kb on + strand, within mngR at 764.515 kb on + strand, within mngR at 764.515 kb on + strand, within mngR at 764.515 kb on + strand, within mngR at 764.515 kb on + strand, within mngR at 764.645 kb on + strand, within mngR at 764.668 kb on + strand, within mngR at 764.811 kb on + strand, within mngR at 765.279 kb on + strand at 765.424 kb on + strand, within mngA at 765.590 kb on + strand, within mngA at 765.590 kb on + strand, within mngA at 765.674 kb on + strand, within mngA at 765.674 kb on - strand, within mngA at 765.675 kb on - strand, within mngA at 765.803 kb on - strand, within mngA at 765.803 kb on - strand, within mngA at 765.811 kb on - strand, within mngA at 765.811 kb on - strand, within mngA at 765.960 kb on - strand, within mngA at 766.059 kb on - strand, within mngA at 766.059 kb on - strand, within mngA at 766.066 kb on - strand, within mngA
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 3 remove 763,625 + sucD b0729 0.26 -0.5 763,625 + sucD b0729 0.26 -1.0 763,712 + sucD b0729 0.36 +0.0 763,712 + sucD b0729 0.36 -0.0 763,773 + sucD b0729 0.43 +0.2 763,871 - sucD b0729 0.54 -0.4 763,901 + sucD b0729 0.57 +0.2 764,252 - -0.5 764,279 + +0.4 764,302 + +0.5 764,302 + -0.1 764,311 + +0.1 764,319 + +0.1 764,349 + -0.6 764,480 + mngR b0730 0.14 -1.8 764,480 + mngR b0730 0.14 +0.9 764,515 + mngR b0730 0.19 -1.4 764,515 + mngR b0730 0.19 +0.8 764,515 + mngR b0730 0.19 -0.4 764,515 + mngR b0730 0.19 +1.4 764,645 + mngR b0730 0.37 -0.9 764,668 + mngR b0730 0.40 -1.3 764,811 + mngR b0730 0.60 +1.8 765,279 + +1.2 765,424 + mngA b0731 0.11 -0.5 765,590 + mngA b0731 0.19 -1.8 765,590 + mngA b0731 0.19 -1.1 765,674 + mngA b0731 0.24 -0.1 765,674 - mngA b0731 0.24 -0.4 765,675 - mngA b0731 0.24 +0.7 765,803 - mngA b0731 0.30 -0.3 765,803 - mngA b0731 0.30 -0.2 765,811 - mngA b0731 0.31 -0.1 765,811 - mngA b0731 0.31 +0.6 765,960 - mngA b0731 0.38 +0.2 766,059 - mngA b0731 0.43 +0.1 766,059 - mngA b0731 0.43 +0.4 766,066 - mngA b0731 0.43 -0.0
Or see this region's nucleotide sequence