Experiment: Ying_Nucleobase14 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ilvI and ilvH are separated by 2 nucleotides ilvH and fruR are separated by 179 nucleotides fruR and mraZ are separated by 601 nucleotides
b0077: ilvI - acetolactate synthase III large subunit (NCBI), at 85,630 to 87,354
ilvI
b0078: ilvH - acetolactate synthase small subunit (NCBI), at 87,357 to 87,848
ilvH
b0080: fruR - DNA-binding transcriptional dual regulator (NCBI), at 88,028 to 89,032
fruR
b0081: mraZ - hypothetical protein (NCBI), at 89,634 to 90,092
mraZ
Position (kb)
88
89
90 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4 at 87.034 kb on - strand, within ilvI at 87.062 kb on - strand, within ilvI at 87.070 kb on + strand, within ilvI at 87.070 kb on + strand, within ilvI at 87.092 kb on - strand, within ilvI at 87.098 kb on - strand, within ilvI at 87.098 kb on - strand, within ilvI at 87.130 kb on + strand, within ilvI at 87.165 kb on - strand, within ilvI at 87.166 kb on - strand, within ilvI at 87.250 kb on + strand at 87.285 kb on + strand at 87.443 kb on - strand, within ilvH at 87.480 kb on + strand, within ilvH at 87.497 kb on + strand, within ilvH at 87.571 kb on + strand, within ilvH at 87.571 kb on + strand, within ilvH at 87.588 kb on - strand, within ilvH at 87.617 kb on - strand, within ilvH at 87.617 kb on - strand, within ilvH at 87.640 kb on + strand, within ilvH at 87.665 kb on - strand, within ilvH at 87.698 kb on - strand, within ilvH at 87.705 kb on + strand, within ilvH at 87.710 kb on + strand, within ilvH at 87.710 kb on + strand, within ilvH at 87.718 kb on - strand, within ilvH at 87.718 kb on - strand, within ilvH at 87.718 kb on - strand, within ilvH at 87.718 kb on - strand, within ilvH at 87.740 kb on - strand, within ilvH at 87.744 kb on - strand, within ilvH at 87.785 kb on + strand, within ilvH at 87.802 kb on + strand at 87.815 kb on - strand at 87.918 kb on - strand at 87.951 kb on - strand at 87.955 kb on + strand at 88.009 kb on + strand at 88.074 kb on + strand at 88.085 kb on + strand at 88.111 kb on - strand at 88.111 kb on - strand at 88.111 kb on - strand at 88.111 kb on - strand at 88.113 kb on - strand at 88.187 kb on + strand, within fruR at 88.187 kb on + strand, within fruR at 88.259 kb on - strand, within fruR at 88.265 kb on + strand, within fruR at 88.365 kb on + strand, within fruR at 88.365 kb on + strand, within fruR at 88.432 kb on + strand, within fruR at 88.432 kb on + strand, within fruR at 88.471 kb on - strand, within fruR at 88.482 kb on + strand, within fruR at 88.490 kb on - strand, within fruR at 88.492 kb on + strand, within fruR at 88.492 kb on + strand, within fruR at 88.492 kb on + strand, within fruR at 88.500 kb on - strand, within fruR at 88.517 kb on - strand, within fruR at 88.517 kb on - strand, within fruR at 88.683 kb on + strand, within fruR at 88.753 kb on + strand, within fruR at 88.772 kb on + strand at 88.774 kb on + strand, within fruR at 88.821 kb on + strand, within fruR at 88.851 kb on + strand, within fruR at 88.851 kb on + strand, within fruR at 88.868 kb on + strand, within fruR at 88.894 kb on + strand, within fruR at 88.937 kb on + strand at 88.945 kb on - strand at 88.945 kb on - strand at 88.946 kb on + strand at 88.949 kb on - strand at 88.992 kb on + strand at 89.000 kb on + strand at 89.001 kb on - strand at 89.012 kb on + strand at 89.015 kb on + strand at 89.015 kb on + strand at 89.017 kb on + strand at 89.022 kb on + strand at 89.023 kb on - strand at 89.023 kb on - strand at 89.023 kb on - strand at 89.079 kb on + strand at 89.079 kb on + strand at 89.079 kb on + strand at 89.079 kb on + strand at 89.079 kb on + strand at 89.079 kb on + strand at 89.086 kb on + strand at 89.087 kb on - strand at 89.087 kb on - strand at 89.087 kb on - strand at 89.087 kb on - strand at 89.087 kb on - strand at 89.087 kb on - strand at 89.089 kb on - strand at 89.089 kb on - strand at 89.089 kb on - strand at 89.089 kb on - strand at 89.091 kb on + strand at 89.122 kb on + strand at 89.132 kb on + strand at 89.225 kb on + strand at 89.294 kb on - strand at 89.307 kb on + strand at 89.307 kb on + strand at 89.312 kb on + strand at 89.312 kb on + strand at 89.312 kb on + strand at 89.313 kb on - strand at 89.315 kb on - strand at 89.325 kb on + strand at 89.325 kb on - strand at 89.330 kb on - strand at 89.391 kb on + strand at 89.393 kb on + strand at 89.401 kb on - strand at 89.411 kb on + strand at 89.411 kb on + strand at 89.416 kb on - strand at 89.508 kb on + strand at 89.508 kb on + strand at 89.542 kb on - strand at 89.542 kb on - strand at 89.550 kb on + strand at 89.558 kb on - strand at 89.649 kb on + strand at 89.649 kb on + strand at 89.658 kb on + strand at 89.680 kb on + strand, within mraZ at 89.685 kb on + strand, within mraZ at 89.917 kb on + strand, within mraZ at 89.917 kb on + strand, within mraZ at 89.981 kb on + strand, within mraZ
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 3 remove 87,034 - ilvI b0077 0.81 -1.2 87,062 - ilvI b0077 0.83 -0.7 87,070 + ilvI b0077 0.83 -0.5 87,070 + ilvI b0077 0.83 -0.3 87,092 - ilvI b0077 0.85 -0.5 87,098 - ilvI b0077 0.85 -0.4 87,098 - ilvI b0077 0.85 -0.8 87,130 + ilvI b0077 0.87 -0.8 87,165 - ilvI b0077 0.89 -1.1 87,166 - ilvI b0077 0.89 -1.6 87,250 + -0.7 87,285 + -0.3 87,443 - ilvH b0078 0.17 +0.2 87,480 + ilvH b0078 0.25 -0.5 87,497 + ilvH b0078 0.28 -0.6 87,571 + ilvH b0078 0.43 -0.7 87,571 + ilvH b0078 0.43 -1.1 87,588 - ilvH b0078 0.47 -1.1 87,617 - ilvH b0078 0.53 -0.3 87,617 - ilvH b0078 0.53 +0.7 87,640 + ilvH b0078 0.58 -0.5 87,665 - ilvH b0078 0.63 -0.5 87,698 - ilvH b0078 0.69 -0.9 87,705 + ilvH b0078 0.71 -1.1 87,710 + ilvH b0078 0.72 -1.1 87,710 + ilvH b0078 0.72 -0.2 87,718 - ilvH b0078 0.73 -0.3 87,718 - ilvH b0078 0.73 -0.3 87,718 - ilvH b0078 0.73 -0.6 87,718 - ilvH b0078 0.73 -0.3 87,740 - ilvH b0078 0.78 -0.9 87,744 - ilvH b0078 0.79 -0.2 87,785 + ilvH b0078 0.87 -0.5 87,802 + +0.3 87,815 - -0.5 87,918 - -0.0 87,951 - -0.5 87,955 + +0.1 88,009 + +0.1 88,074 + -6.2 88,085 + -1.4 88,111 - -1.9 88,111 - -1.7 88,111 - -2.1 88,111 - -3.1 88,113 - -4.7 88,187 + fruR b0080 0.16 -3.8 88,187 + fruR b0080 0.16 -3.0 88,259 - fruR b0080 0.23 -4.2 88,265 + fruR b0080 0.24 -3.6 88,365 + fruR b0080 0.34 -3.9 88,365 + fruR b0080 0.34 -3.6 88,432 + fruR b0080 0.40 -3.7 88,432 + fruR b0080 0.40 -3.5 88,471 - fruR b0080 0.44 -3.2 88,482 + fruR b0080 0.45 -3.7 88,490 - fruR b0080 0.46 -5.0 88,492 + fruR b0080 0.46 -1.6 88,492 + fruR b0080 0.46 -1.8 88,492 + fruR b0080 0.46 -4.3 88,500 - fruR b0080 0.47 -3.7 88,517 - fruR b0080 0.49 -2.1 88,517 - fruR b0080 0.49 -3.0 88,683 + fruR b0080 0.65 -4.2 88,753 + fruR b0080 0.72 -3.2 88,772 + +0.2 88,774 + fruR b0080 0.74 -3.3 88,821 + fruR b0080 0.79 -4.0 88,851 + fruR b0080 0.82 -2.9 88,851 + fruR b0080 0.82 -3.4 88,868 + fruR b0080 0.84 -4.0 88,894 + fruR b0080 0.86 -3.6 88,937 + -3.5 88,945 - -3.0 88,945 - -1.9 88,946 + -4.9 88,949 - -4.1 88,992 + -6.0 89,000 + -1.7 89,001 - -3.1 89,012 + -4.0 89,015 + -3.2 89,015 + -4.9 89,017 + -0.8 89,022 + +0.1 89,023 - +0.4 89,023 - -3.3 89,023 - -4.6 89,079 + +0.1 89,079 + +0.3 89,079 + -1.2 89,079 + +0.0 89,079 + +0.7 89,079 + -0.6 89,086 + +0.5 89,087 - +3.8 89,087 - +1.2 89,087 - -0.2 89,087 - -0.2 89,087 - +1.4 89,087 - -0.6 89,089 - +1.2 89,089 - -0.0 89,089 - +0.3 89,089 - -0.8 89,091 + +0.3 89,122 + -0.1 89,132 + -0.1 89,225 + -0.5 89,294 - -0.3 89,307 + +0.1 89,307 + +0.2 89,312 + -0.9 89,312 + -0.8 89,312 + -0.6 89,313 - -0.6 89,315 - +0.5 89,325 + +0.0 89,325 - -1.2 89,330 - -0.2 89,391 + +0.1 89,393 + +0.4 89,401 - +0.4 89,411 + -0.3 89,411 + -1.0 89,416 - -0.3 89,508 + +0.4 89,508 + -0.6 89,542 - +0.0 89,542 - +0.4 89,550 + -1.3 89,558 - +0.0 89,649 + -1.1 89,649 + -0.5 89,658 + -0.0 89,680 + mraZ b0081 0.10 -1.7 89,685 + mraZ b0081 0.11 -1.0 89,917 + mraZ b0081 0.62 -1.3 89,917 + mraZ b0081 0.62 -0.9 89,981 + mraZ b0081 0.76 -1.8
Or see this region's nucleotide sequence