Experiment: Ying_Nucleobase14 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kefC and folA are separated by 191 nucleotides folA and apaH are separated by 77 nucleotides apaH and apaG are separated by 6 nucleotides
b0047: kefC - glutathione-regulated potassium-efflux system protein (NCBI), at 47,769 to 49,631
kefC
b0048: folA - dihydrofolate reductase (NCBI), at 49,823 to 50,302
folA
b0049: apaH - diadenosinetetraphosphatase (NCBI), at 50,380 to 51,222
apaH
b0050: apaG - hypothetical protein (NCBI), at 51,229 to 51,606
apaG
Position (kb)
49
50
51 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 48.907 kb on + strand, within kefC at 48.958 kb on + strand, within kefC at 48.968 kb on - strand, within kefC at 49.068 kb on - strand, within kefC at 49.068 kb on - strand, within kefC at 49.149 kb on - strand, within kefC at 49.149 kb on - strand, within kefC at 49.202 kb on + strand, within kefC at 49.281 kb on + strand, within kefC at 49.289 kb on - strand, within kefC at 49.289 kb on - strand, within kefC at 49.334 kb on + strand, within kefC at 49.334 kb on + strand, within kefC at 49.356 kb on - strand, within kefC at 49.356 kb on - strand, within kefC at 49.369 kb on + strand, within kefC at 49.369 kb on + strand, within kefC at 49.409 kb on - strand, within kefC at 49.431 kb on + strand, within kefC at 49.487 kb on - strand at 49.493 kb on - strand at 49.709 kb on + strand at 50.361 kb on + strand at 50.361 kb on + strand at 50.370 kb on - strand at 50.376 kb on + strand at 50.461 kb on + strand at 50.469 kb on - strand, within apaH at 50.501 kb on + strand, within apaH at 50.507 kb on - strand, within apaH at 50.568 kb on - strand, within apaH at 50.717 kb on - strand, within apaH at 50.785 kb on - strand, within apaH at 50.791 kb on + strand, within apaH at 50.802 kb on + strand, within apaH at 50.829 kb on - strand, within apaH at 50.859 kb on - strand, within apaH at 50.877 kb on + strand, within apaH at 50.877 kb on + strand, within apaH at 51.025 kb on + strand, within apaH at 51.109 kb on - strand, within apaH at 51.177 kb on + strand at 51.177 kb on + strand at 51.185 kb on - strand at 51.190 kb on + strand at 51.190 kb on + strand at 51.240 kb on + strand at 51.240 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 3 remove 48,907 + kefC b0047 0.61 -0.2 48,958 + kefC b0047 0.64 +0.8 48,968 - kefC b0047 0.64 +0.6 49,068 - kefC b0047 0.70 -0.3 49,068 - kefC b0047 0.70 -0.1 49,149 - kefC b0047 0.74 +0.8 49,149 - kefC b0047 0.74 +0.7 49,202 + kefC b0047 0.77 +0.2 49,281 + kefC b0047 0.81 +0.1 49,289 - kefC b0047 0.82 -1.4 49,289 - kefC b0047 0.82 +0.2 49,334 + kefC b0047 0.84 -1.8 49,334 + kefC b0047 0.84 +0.3 49,356 - kefC b0047 0.85 -0.2 49,356 - kefC b0047 0.85 -1.5 49,369 + kefC b0047 0.86 +0.1 49,369 + kefC b0047 0.86 -0.0 49,409 - kefC b0047 0.88 -0.0 49,431 + kefC b0047 0.89 -0.3 49,487 - +0.2 49,493 - +0.5 49,709 + -0.2 50,361 + +0.2 50,361 + +0.2 50,370 - -0.3 50,376 + +0.1 50,461 + -1.8 50,469 - apaH b0049 0.11 -1.9 50,501 + apaH b0049 0.14 -1.4 50,507 - apaH b0049 0.15 -1.8 50,568 - apaH b0049 0.22 -1.6 50,717 - apaH b0049 0.40 -2.9 50,785 - apaH b0049 0.48 -2.9 50,791 + apaH b0049 0.49 -0.9 50,802 + apaH b0049 0.50 -2.8 50,829 - apaH b0049 0.53 -0.6 50,859 - apaH b0049 0.57 -0.8 50,877 + apaH b0049 0.59 +0.2 50,877 + apaH b0049 0.59 -0.4 51,025 + apaH b0049 0.77 -2.2 51,109 - apaH b0049 0.86 -3.7 51,177 + -1.6 51,177 + -1.1 51,185 - -2.7 51,190 + -3.0 51,190 + -1.7 51,240 + +0.0 51,240 + -0.4
Or see this region's nucleotide sequence