Experiment: Ying_Nucleobase14 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt entE and entB are separated by 13 nucleotides entB and entA overlap by 1 nucleotides entA and ybdB are separated by 2 nucleotides
b0594: entE - 2,3-dihydroxybenzoate-AMP ligase component of enterobactin synthase multienzyme complex (NCBI), at 625,293 to 626,903
entE
b0595: entB - isochorismatase (NCBI), at 626,917 to 627,774
entB
b0596: entA - 2,3-dihydroxybenzoate-2,3-dehydrogenase (NCBI), at 627,774 to 628,520
entA
b0597: ybdB - hypothetical protein (NCBI), at 628,523 to 628,936
ybdB
Position (kb) 626
627
628 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 625.958 kb on + strand, within entE at 625.964 kb on - strand, within entE at 625.964 kb on - strand, within entE at 625.971 kb on - strand, within entE at 626.073 kb on + strand, within entE at 626.303 kb on - strand, within entE at 626.303 kb on - strand, within entE at 626.369 kb on - strand, within entE at 626.487 kb on - strand, within entE at 626.643 kb on + strand, within entE at 626.643 kb on + strand, within entE at 626.751 kb on + strand at 626.751 kb on + strand at 626.851 kb on - strand at 626.896 kb on - strand at 626.908 kb on - strand at 626.908 kb on - strand at 626.937 kb on + strand at 626.937 kb on + strand at 627.113 kb on + strand, within entB at 627.113 kb on + strand, within entB at 627.128 kb on + strand, within entB at 627.128 kb on + strand, within entB at 627.146 kb on - strand, within entB at 627.146 kb on - strand, within entB at 627.146 kb on - strand, within entB at 627.146 kb on - strand, within entB at 627.216 kb on - strand, within entB at 627.216 kb on - strand, within entB at 627.367 kb on + strand, within entB at 627.437 kb on - strand, within entB at 627.437 kb on - strand, within entB at 627.550 kb on - strand, within entB at 627.610 kb on - strand, within entB at 627.619 kb on + strand, within entB at 627.619 kb on + strand, within entB at 627.629 kb on - strand, within entB at 627.706 kb on - strand at 627.751 kb on + strand at 627.825 kb on + strand at 627.950 kb on - strand, within entA at 628.176 kb on + strand, within entA at 628.200 kb on + strand, within entA at 628.208 kb on - strand, within entA at 628.219 kb on - strand, within entA at 628.257 kb on - strand, within entA at 628.287 kb on - strand, within entA at 628.287 kb on - strand, within entA at 628.297 kb on + strand, within entA at 628.297 kb on + strand, within entA at 628.319 kb on - strand, within entA at 628.387 kb on - strand, within entA at 628.463 kb on - strand at 628.463 kb on - strand at 628.601 kb on + strand, within ybdB at 628.609 kb on - strand, within ybdB at 628.618 kb on - strand, within ybdB at 628.640 kb on + strand, within ybdB at 628.640 kb on + strand, within ybdB at 628.756 kb on + strand, within ybdB at 628.756 kb on + strand, within ybdB
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 3 remove 625,958 + entE b0594 0.41 -1.7 625,964 - entE b0594 0.42 -3.6 625,964 - entE b0594 0.42 -0.1 625,971 - entE b0594 0.42 -0.4 626,073 + entE b0594 0.48 -0.5 626,303 - entE b0594 0.63 +0.1 626,303 - entE b0594 0.63 -0.4 626,369 - entE b0594 0.67 -0.5 626,487 - entE b0594 0.74 -1.3 626,643 + entE b0594 0.84 -1.2 626,643 + entE b0594 0.84 -1.5 626,751 + -0.7 626,751 + -0.3 626,851 - -0.2 626,896 - -1.1 626,908 - +0.8 626,908 - -0.2 626,937 + -1.0 626,937 + -1.2 627,113 + entB b0595 0.23 -0.3 627,113 + entB b0595 0.23 +1.2 627,128 + entB b0595 0.25 -1.0 627,128 + entB b0595 0.25 -2.0 627,146 - entB b0595 0.27 -2.0 627,146 - entB b0595 0.27 -0.9 627,146 - entB b0595 0.27 -0.6 627,146 - entB b0595 0.27 -2.8 627,216 - entB b0595 0.35 -0.9 627,216 - entB b0595 0.35 -1.2 627,367 + entB b0595 0.52 -0.9 627,437 - entB b0595 0.61 -1.1 627,437 - entB b0595 0.61 -0.1 627,550 - entB b0595 0.74 -1.4 627,610 - entB b0595 0.81 +1.2 627,619 + entB b0595 0.82 -0.3 627,619 + entB b0595 0.82 -0.3 627,629 - entB b0595 0.83 -0.1 627,706 - +0.0 627,751 + -0.3 627,825 + -0.8 627,950 - entA b0596 0.24 -0.4 628,176 + entA b0596 0.54 -0.2 628,200 + entA b0596 0.57 -3.0 628,208 - entA b0596 0.58 -0.2 628,219 - entA b0596 0.60 -1.0 628,257 - entA b0596 0.65 -0.8 628,287 - entA b0596 0.69 -0.2 628,287 - entA b0596 0.69 +0.0 628,297 + entA b0596 0.70 +0.1 628,297 + entA b0596 0.70 -1.4 628,319 - entA b0596 0.73 -0.1 628,387 - entA b0596 0.82 +0.1 628,463 - -0.3 628,463 - -0.9 628,601 + ybdB b0597 0.19 -0.2 628,609 - ybdB b0597 0.21 +0.2 628,618 - ybdB b0597 0.23 -1.9 628,640 + ybdB b0597 0.28 +0.1 628,640 + ybdB b0597 0.28 -3.9 628,756 + ybdB b0597 0.56 -0.6 628,756 + ybdB b0597 0.56 -0.1
Or see this region's nucleotide sequence