Experiment: Ying_Nucleobase14 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt proS and yaeB are separated by 111 nucleotides yaeB and rcsF overlap by 4 nucleotides rcsF and metQ are separated by 117 nucleotides metQ and metI are separated by 39 nucleotides
b0194: proS - prolyl-tRNA synthetase (NCBI), at 217,057 to 218,775
proS
b0195: yaeB - hypothetical protein (NCBI), at 218,887 to 219,594
yaeB
b0196: rcsF - predicted outer membrane protein, signal (NCBI), at 219,591 to 219,995
rcsF
b0197: metQ - DL-methionine transporter subunit (NCBI), at 220,113 to 220,928
metQ
b0198: metI - DL-methionine transporter subunit (NCBI), at 220,968 to 221,621
metI
Position (kb) 219
220 Strain fitness (log2 ratio) -1
0
1 at 218.821 kb on - strand at 218.871 kb on + strand at 218.910 kb on + strand at 218.910 kb on + strand at 218.965 kb on + strand, within yaeB at 218.974 kb on - strand, within yaeB at 219.027 kb on - strand, within yaeB at 219.071 kb on - strand, within yaeB at 219.200 kb on + strand, within yaeB at 219.240 kb on + strand, within yaeB at 219.240 kb on + strand, within yaeB at 219.261 kb on + strand, within yaeB at 219.312 kb on + strand, within yaeB at 219.446 kb on - strand, within yaeB at 219.465 kb on + strand, within yaeB at 219.466 kb on + strand, within yaeB at 219.489 kb on - strand, within yaeB at 219.513 kb on + strand, within yaeB at 219.548 kb on - strand at 219.598 kb on + strand at 219.598 kb on + strand at 219.643 kb on - strand, within rcsF at 219.678 kb on + strand, within rcsF at 219.680 kb on + strand, within rcsF at 219.680 kb on + strand, within rcsF at 219.680 kb on + strand, within rcsF at 219.720 kb on - strand, within rcsF at 219.720 kb on - strand, within rcsF at 219.756 kb on + strand, within rcsF at 219.764 kb on - strand, within rcsF at 219.764 kb on - strand, within rcsF at 219.764 kb on - strand, within rcsF at 219.842 kb on - strand, within rcsF at 219.902 kb on + strand, within rcsF at 219.944 kb on - strand, within rcsF at 219.968 kb on + strand at 219.976 kb on - strand at 220.041 kb on - strand at 220.041 kb on - strand at 220.041 kb on - strand at 220.127 kb on - strand at 220.127 kb on - strand at 220.249 kb on - strand, within metQ at 220.321 kb on + strand, within metQ at 220.321 kb on + strand, within metQ at 220.323 kb on + strand, within metQ at 220.361 kb on - strand, within metQ at 220.386 kb on - strand, within metQ at 220.386 kb on - strand, within metQ at 220.405 kb on + strand, within metQ at 220.405 kb on + strand, within metQ at 220.432 kb on + strand, within metQ at 220.432 kb on + strand, within metQ at 220.504 kb on + strand, within metQ at 220.532 kb on + strand, within metQ at 220.543 kb on + strand, within metQ at 220.661 kb on + strand, within metQ at 220.675 kb on + strand, within metQ at 220.694 kb on - strand, within metQ at 220.694 kb on - strand, within metQ at 220.750 kb on - strand, within metQ at 220.754 kb on + strand, within metQ at 220.764 kb on + strand, within metQ at 220.825 kb on + strand, within metQ at 220.865 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 3 remove 218,821 - +0.7 218,871 + -0.2 218,910 + +1.3 218,910 + -0.3 218,965 + yaeB b0195 0.11 +0.6 218,974 - yaeB b0195 0.12 +0.4 219,027 - yaeB b0195 0.20 -0.2 219,071 - yaeB b0195 0.26 +0.7 219,200 + yaeB b0195 0.44 +0.9 219,240 + yaeB b0195 0.50 -0.3 219,240 + yaeB b0195 0.50 +0.3 219,261 + yaeB b0195 0.53 -0.4 219,312 + yaeB b0195 0.60 -0.5 219,446 - yaeB b0195 0.79 +0.0 219,465 + yaeB b0195 0.82 +0.7 219,466 + yaeB b0195 0.82 -0.3 219,489 - yaeB b0195 0.85 +0.2 219,513 + yaeB b0195 0.88 +0.6 219,548 - +0.3 219,598 + -1.0 219,598 + +0.4 219,643 - rcsF b0196 0.13 -0.2 219,678 + rcsF b0196 0.21 -0.2 219,680 + rcsF b0196 0.22 -0.4 219,680 + rcsF b0196 0.22 +0.4 219,680 + rcsF b0196 0.22 -0.6 219,720 - rcsF b0196 0.32 -0.7 219,720 - rcsF b0196 0.32 -0.1 219,756 + rcsF b0196 0.41 +1.0 219,764 - rcsF b0196 0.43 +0.2 219,764 - rcsF b0196 0.43 +0.6 219,764 - rcsF b0196 0.43 +0.4 219,842 - rcsF b0196 0.62 -1.1 219,902 + rcsF b0196 0.77 +0.9 219,944 - rcsF b0196 0.87 +0.4 219,968 + +0.7 219,976 - -0.4 220,041 - +1.2 220,041 - +0.7 220,041 - +0.5 220,127 - +1.3 220,127 - +0.5 220,249 - metQ b0197 0.17 +1.3 220,321 + metQ b0197 0.25 +1.2 220,321 + metQ b0197 0.25 +1.4 220,323 + metQ b0197 0.26 +1.1 220,361 - metQ b0197 0.30 +0.8 220,386 - metQ b0197 0.33 +0.6 220,386 - metQ b0197 0.33 +1.1 220,405 + metQ b0197 0.36 +1.3 220,405 + metQ b0197 0.36 +0.7 220,432 + metQ b0197 0.39 +1.2 220,432 + metQ b0197 0.39 +1.3 220,504 + metQ b0197 0.48 +1.7 220,532 + metQ b0197 0.51 +0.7 220,543 + metQ b0197 0.53 +0.5 220,661 + metQ b0197 0.67 +1.6 220,675 + metQ b0197 0.69 +0.9 220,694 - metQ b0197 0.71 +1.0 220,694 - metQ b0197 0.71 +0.5 220,750 - metQ b0197 0.78 +0.9 220,754 + metQ b0197 0.79 +1.1 220,764 + metQ b0197 0.80 +1.0 220,825 + metQ b0197 0.87 +1.7 220,865 + +1.7
Or see this region's nucleotide sequence