Strain Fitness in Escherichia coli BW25113 around b0002

Experiment: Ying_Nucleobase14 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthrL and thrA are separated by 81 nucleotidesthrA and thrB are separated by 1 nucleotidesthrB and thrC are separated by 0 nucleotides b0001: thrL - thr operon leader peptide (NCBI), at 190 to 255 thrL b0002: thrA - bifunctional aspartokinase I/homeserine dehydrogenase I (NCBI), at 337 to 2,799 thrA b0003: thrB - homoserine kinase (NCBI), at 2,801 to 3,733 thrB b0004: thrC - threonine synthase (NCBI), at 3,734 to 5,020 thrC Position (kb) 0 1 2 3Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1at 0.070 kb on + strandat 0.070 kb on + strandat 0.078 kb on - strandat 0.078 kb on - strandat 0.161 kb on - strandat 0.185 kb on - strandat 0.185 kb on - strandat 0.208 kb on + strand, within thrLat 0.217 kb on - strand, within thrLat 0.257 kb on - strandat 0.257 kb on - strandat 0.440 kb on - strandat 0.440 kb on - strandat 0.454 kb on - strandat 0.518 kb on - strandat 0.518 kb on - strandat 0.528 kb on + strandat 0.592 kb on + strand, within thrAat 0.592 kb on + strand, within thrAat 0.666 kb on + strand, within thrAat 0.666 kb on + strand, within thrAat 0.675 kb on - strand, within thrAat 0.754 kb on - strand, within thrAat 0.754 kb on - strand, within thrAat 0.767 kb on + strand, within thrAat 0.775 kb on - strand, within thrAat 0.957 kb on + strand, within thrAat 0.957 kb on + strand, within thrAat 1.046 kb on + strand, within thrAat 1.136 kb on - strand, within thrAat 1.142 kb on - strand, within thrAat 1.161 kb on + strand, within thrAat 1.161 kb on + strand, within thrAat 1.161 kb on + strand, within thrAat 1.259 kb on - strand, within thrAat 1.259 kb on - strand, within thrAat 1.262 kb on + strand, within thrAat 1.270 kb on - strand, within thrAat 1.270 kb on - strand, within thrAat 1.280 kb on - strand, within thrAat 1.489 kb on + strand, within thrAat 1.599 kb on - strand, within thrAat 1.606 kb on + strand, within thrAat 1.627 kb on + strand, within thrAat 1.633 kb on - strand, within thrAat 1.633 kb on - strand, within thrAat 1.636 kb on + strand, within thrAat 1.636 kb on + strand, within thrAat 1.637 kb on + strand, within thrAat 1.711 kb on - strand, within thrAat 1.721 kb on - strand, within thrAat 1.767 kb on - strand, within thrAat 1.793 kb on - strand, within thrAat 1.868 kb on + strand, within thrAat 1.868 kb on + strand, within thrAat 1.871 kb on + strand, within thrAat 1.876 kb on - strand, within thrAat 1.881 kb on - strand, within thrAat 1.881 kb on - strand, within thrAat 1.929 kb on + strand, within thrAat 1.929 kb on + strand, within thrAat 1.937 kb on - strand, within thrAat 1.937 kb on - strand, within thrAat 2.076 kb on + strand, within thrAat 2.104 kb on + strand, within thrAat 2.104 kb on + strand, within thrAat 2.266 kb on - strand, within thrAat 2.266 kb on - strand, within thrAat 2.292 kb on - strand, within thrAat 2.294 kb on + strand, within thrAat 2.370 kb on - strand, within thrAat 2.370 kb on - strand, within thrAat 2.449 kb on - strand, within thrAat 2.590 kb on - strandat 2.675 kb on + strandat 2.682 kb on + strandat 2.682 kb on + strandat 2.684 kb on + strandat 2.717 kb on + strandat 2.744 kb on - strandat 2.787 kb on - strandat 2.787 kb on - strandat 2.829 kb on + strandat 2.847 kb on + strandat 2.847 kb on + strandat 2.906 kb on - strand, within thrBat 2.934 kb on + strand, within thrBat 2.963 kb on - strand, within thrBat 3.020 kb on - strand, within thrBat 3.023 kb on + strand, within thrBat 3.131 kb on + strand, within thrBat 3.144 kb on - strand, within thrBat 3.186 kb on + strand, within thrBat 3.246 kb on + strand, within thrBat 3.248 kb on + strand, within thrBat 3.256 kb on + strand, within thrBat 3.293 kb on + strand, within thrBat 3.301 kb on - strand, within thrBat 3.318 kb on - strand, within thrBat 3.411 kb on + strand, within thrBat 3.444 kb on - strand, within thrBat 3.444 kb on - strand, within thrBat 3.504 kb on - strand, within thrBat 3.504 kb on - strand, within thrBat 3.509 kb on - strand, within thrBat 3.511 kb on + strand, within thrBat 3.607 kb on + strand, within thrBat 3.634 kb on - strand, within thrBat 3.638 kb on + strand, within thrBat 3.638 kb on + strand, within thrBat 3.650 kb on + strandat 3.769 kb on + strandat 3.769 kb on + strandat 3.792 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 3
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70 + -0.1
70 + -0.2
78 - +0.1
78 - -2.6
161 - -4.2
185 - -2.7
185 - -2.3
208 + thrL b0001 0.27 -0.0
217 - thrL b0001 0.41 -4.7
257 - -2.0
257 - -4.5
440 - -4.8
440 - -4.8
454 - -4.6
518 - -2.8
518 - -4.5
528 + -3.9
592 + thrA b0002 0.10 -4.2
592 + thrA b0002 0.10 -2.9
666 + thrA b0002 0.13 -3.9
666 + thrA b0002 0.13 -3.4
675 - thrA b0002 0.14 -6.2
754 - thrA b0002 0.17 -5.6
754 - thrA b0002 0.17 -4.6
767 + thrA b0002 0.17 -4.7
775 - thrA b0002 0.18 -4.5
957 + thrA b0002 0.25 -4.2
957 + thrA b0002 0.25 -2.4
1,046 + thrA b0002 0.29 -3.8
1,136 - thrA b0002 0.32 -6.5
1,142 - thrA b0002 0.33 -6.0
1,161 + thrA b0002 0.33 -4.4
1,161 + thrA b0002 0.33 -4.9
1,161 + thrA b0002 0.33 -4.6
1,259 - thrA b0002 0.37 -5.8
1,259 - thrA b0002 0.37 -5.5
1,262 + thrA b0002 0.38 -6.9
1,270 - thrA b0002 0.38 -4.1
1,270 - thrA b0002 0.38 -4.0
1,280 - thrA b0002 0.38 -4.3
1,489 + thrA b0002 0.47 -4.8
1,599 - thrA b0002 0.51 -4.3
1,606 + thrA b0002 0.52 -5.2
1,627 + thrA b0002 0.52 -4.9
1,633 - thrA b0002 0.53 -4.5
1,633 - thrA b0002 0.53 -4.5
1,636 + thrA b0002 0.53 -3.7
1,636 + thrA b0002 0.53 -2.8
1,637 + thrA b0002 0.53 -5.7
1,711 - thrA b0002 0.56 -3.1
1,721 - thrA b0002 0.56 -4.2
1,767 - thrA b0002 0.58 -5.3
1,793 - thrA b0002 0.59 -6.8
1,868 + thrA b0002 0.62 -4.4
1,868 + thrA b0002 0.62 -4.8
1,871 + thrA b0002 0.62 -4.7
1,876 - thrA b0002 0.62 -3.6
1,881 - thrA b0002 0.63 -4.9
1,881 - thrA b0002 0.63 -3.5
1,929 + thrA b0002 0.65 -4.7
1,929 + thrA b0002 0.65 -5.0
1,937 - thrA b0002 0.65 -2.1
1,937 - thrA b0002 0.65 -1.3
2,076 + thrA b0002 0.71 -6.8
2,104 + thrA b0002 0.72 -4.6
2,104 + thrA b0002 0.72 -3.7
2,266 - thrA b0002 0.78 -3.6
2,266 - thrA b0002 0.78 -4.1
2,292 - thrA b0002 0.79 -4.8
2,294 + thrA b0002 0.79 -5.6
2,370 - thrA b0002 0.83 -3.6
2,370 - thrA b0002 0.83 -2.9
2,449 - thrA b0002 0.86 -5.6
2,590 - -3.4
2,675 + -4.2
2,682 + -4.0
2,682 + -0.3
2,684 + -4.1
2,717 + -5.1
2,744 - -4.8
2,787 - -4.0
2,787 - -3.4
2,829 + -5.5
2,847 + -3.6
2,847 + -3.2
2,906 - thrB b0003 0.11 -3.5
2,934 + thrB b0003 0.14 -6.3
2,963 - thrB b0003 0.17 -4.9
3,020 - thrB b0003 0.23 +1.1
3,023 + thrB b0003 0.24 -4.4
3,131 + thrB b0003 0.35 -6.6
3,144 - thrB b0003 0.37 -4.1
3,186 + thrB b0003 0.41 -5.6
3,246 + thrB b0003 0.48 -3.8
3,248 + thrB b0003 0.48 -5.9
3,256 + thrB b0003 0.49 -5.8
3,293 + thrB b0003 0.53 -3.4
3,301 - thrB b0003 0.54 -3.4
3,318 - thrB b0003 0.55 -5.8
3,411 + thrB b0003 0.65 -4.6
3,444 - thrB b0003 0.69 -4.5
3,444 - thrB b0003 0.69 -2.4
3,504 - thrB b0003 0.75 -5.8
3,504 - thrB b0003 0.75 -3.7
3,509 - thrB b0003 0.76 -6.3
3,511 + thrB b0003 0.76 -4.5
3,607 + thrB b0003 0.86 -4.6
3,634 - thrB b0003 0.89 -3.6
3,638 + thrB b0003 0.90 -5.4
3,638 + thrB b0003 0.90 -3.2
3,650 + -3.6
3,769 + -3.6
3,769 + -6.0
3,792 - -4.7

Or see this region's nucleotide sequence