Experiment: Ying_Nucleobase14 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpR and yjjX are separated by 146 nucleotides yjjX and ytjC are separated by 51 nucleotides ytjC and rob overlap by 4 nucleotides
b4393: trpR - Trp operon repressor (NCBI), at 4,630,783 to 4,631,109
trpR
b4394: yjjX - orf, hypothetical protein (VIMSS), at 4,631,256 to 4,631,768
yjjX
b4395: ytjC - phosphoglycerate mutase (NCBI), at 4,631,820 to 4,632,467
ytjC
b4396: rob - DNA-binding transcriptional activator (NCBI), at 4,632,464 to 4,633,333
rob
Position (kb)
4631
4632
4633 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4630.865 kb on - strand, within trpR at 4630.865 kb on - strand, within trpR at 4630.865 kb on - strand, within trpR at 4630.941 kb on - strand, within trpR at 4630.941 kb on - strand, within trpR at 4630.949 kb on + strand, within trpR at 4630.952 kb on - strand, within trpR at 4630.960 kb on + strand, within trpR at 4630.992 kb on + strand, within trpR at 4630.992 kb on + strand, within trpR at 4631.035 kb on + strand, within trpR at 4631.035 kb on + strand, within trpR at 4631.225 kb on + strand at 4631.311 kb on - strand, within yjjX at 4631.311 kb on - strand, within yjjX at 4631.364 kb on - strand, within yjjX at 4631.364 kb on - strand, within yjjX at 4631.520 kb on + strand, within yjjX at 4631.537 kb on - strand, within yjjX at 4631.871 kb on - strand at 4631.878 kb on + strand at 4631.965 kb on + strand, within ytjC at 4631.965 kb on + strand, within ytjC at 4631.991 kb on - strand, within ytjC at 4632.026 kb on - strand, within ytjC at 4632.026 kb on - strand, within ytjC at 4632.026 kb on - strand, within ytjC at 4632.255 kb on + strand, within ytjC at 4632.357 kb on - strand, within ytjC at 4632.357 kb on - strand, within ytjC at 4632.395 kb on + strand, within ytjC at 4632.439 kb on + strand at 4632.439 kb on + strand at 4632.444 kb on + strand at 4632.444 kb on + strand at 4632.447 kb on - strand at 4632.447 kb on - strand at 4632.520 kb on + strand at 4632.520 kb on + strand at 4632.545 kb on - strand at 4632.550 kb on - strand at 4632.567 kb on - strand, within rob at 4632.569 kb on - strand, within rob at 4632.579 kb on - strand, within rob at 4632.583 kb on + strand, within rob at 4632.609 kb on - strand, within rob at 4632.650 kb on + strand, within rob at 4632.664 kb on - strand, within rob at 4632.664 kb on - strand, within rob at 4632.700 kb on + strand, within rob at 4632.708 kb on - strand, within rob at 4632.708 kb on - strand, within rob at 4632.805 kb on - strand, within rob at 4632.883 kb on + strand, within rob at 4632.883 kb on + strand, within rob at 4632.907 kb on + strand, within rob at 4632.910 kb on - strand, within rob at 4632.915 kb on - strand, within rob at 4632.915 kb on - strand, within rob at 4632.931 kb on + strand, within rob at 4632.932 kb on + strand, within rob at 4633.037 kb on + strand, within rob at 4633.037 kb on + strand, within rob at 4633.038 kb on - strand, within rob at 4633.089 kb on + strand, within rob at 4633.089 kb on + strand, within rob at 4633.102 kb on - strand, within rob at 4633.102 kb on - strand, within rob at 4633.193 kb on + strand, within rob at 4633.193 kb on + strand, within rob at 4633.193 kb on + strand, within rob at 4633.227 kb on - strand, within rob at 4633.227 kb on - strand, within rob at 4633.244 kb on - strand, within rob at 4633.274 kb on - strand at 4633.306 kb on - strand at 4633.317 kb on + strand at 4633.364 kb on - strand at 4633.367 kb on + strand at 4633.441 kb on - strand at 4633.445 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 3 remove 4,630,865 - trpR b4393 0.25 -0.7 4,630,865 - trpR b4393 0.25 -1.2 4,630,865 - trpR b4393 0.25 -0.7 4,630,941 - trpR b4393 0.48 -0.5 4,630,941 - trpR b4393 0.48 -0.1 4,630,949 + trpR b4393 0.51 -0.4 4,630,952 - trpR b4393 0.52 -0.8 4,630,960 + trpR b4393 0.54 -1.4 4,630,992 + trpR b4393 0.64 -0.1 4,630,992 + trpR b4393 0.64 -0.2 4,631,035 + trpR b4393 0.77 -0.8 4,631,035 + trpR b4393 0.77 -0.2 4,631,225 + -0.4 4,631,311 - yjjX b4394 0.11 +0.0 4,631,311 - yjjX b4394 0.11 +0.3 4,631,364 - yjjX b4394 0.21 +0.5 4,631,364 - yjjX b4394 0.21 +0.1 4,631,520 + yjjX b4394 0.51 +0.7 4,631,537 - yjjX b4394 0.55 +0.6 4,631,871 - -1.6 4,631,878 + -1.8 4,631,965 + ytjC b4395 0.22 -2.0 4,631,965 + ytjC b4395 0.22 -1.6 4,631,991 - ytjC b4395 0.26 -1.7 4,632,026 - ytjC b4395 0.32 -0.4 4,632,026 - ytjC b4395 0.32 -1.3 4,632,026 - ytjC b4395 0.32 -1.7 4,632,255 + ytjC b4395 0.67 -1.7 4,632,357 - ytjC b4395 0.83 -1.8 4,632,357 - ytjC b4395 0.83 -1.0 4,632,395 + ytjC b4395 0.89 -0.6 4,632,439 + -0.3 4,632,439 + -0.7 4,632,444 + -0.6 4,632,444 + -1.7 4,632,447 - -0.3 4,632,447 - -2.2 4,632,520 + +0.0 4,632,520 + -0.3 4,632,545 - -1.9 4,632,550 - -1.0 4,632,567 - rob b4396 0.12 -1.3 4,632,569 - rob b4396 0.12 -1.1 4,632,579 - rob b4396 0.13 -0.6 4,632,583 + rob b4396 0.14 -0.8 4,632,609 - rob b4396 0.17 -0.8 4,632,650 + rob b4396 0.21 -0.8 4,632,664 - rob b4396 0.23 -1.0 4,632,664 - rob b4396 0.23 -0.7 4,632,700 + rob b4396 0.27 -0.9 4,632,708 - rob b4396 0.28 -1.1 4,632,708 - rob b4396 0.28 -1.7 4,632,805 - rob b4396 0.39 -1.2 4,632,883 + rob b4396 0.48 -1.5 4,632,883 + rob b4396 0.48 -2.1 4,632,907 + rob b4396 0.51 -0.6 4,632,910 - rob b4396 0.51 -0.7 4,632,915 - rob b4396 0.52 -2.1 4,632,915 - rob b4396 0.52 -2.0 4,632,931 + rob b4396 0.54 -1.3 4,632,932 + rob b4396 0.54 -1.2 4,633,037 + rob b4396 0.66 -0.2 4,633,037 + rob b4396 0.66 +0.0 4,633,038 - rob b4396 0.66 -1.5 4,633,089 + rob b4396 0.72 -0.2 4,633,089 + rob b4396 0.72 -1.1 4,633,102 - rob b4396 0.73 -0.6 4,633,102 - rob b4396 0.73 -0.3 4,633,193 + rob b4396 0.84 -0.5 4,633,193 + rob b4396 0.84 -0.9 4,633,193 + rob b4396 0.84 -3.0 4,633,227 - rob b4396 0.88 -0.4 4,633,227 - rob b4396 0.88 +0.0 4,633,244 - rob b4396 0.90 -0.8 4,633,274 - -0.9 4,633,306 - -0.6 4,633,317 + -0.9 4,633,364 - +0.6 4,633,367 + -1.5 4,633,441 - +0.9 4,633,445 - +1.4
Or see this region's nucleotide sequence