Experiment: Ying_Nucleobase14 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nlpD and pcm are separated by 139 nucleotides pcm and surE overlap by 7 nucleotides surE and truD overlap by 20 nucleotides
b2742: nlpD - predicted outer membrane lipoprotein (NCBI), at 2,865,636 to 2,866,775
nlpD
b2743: pcm - protein-L-isoaspartate O-methyltransferase (NCBI), at 2,866,915 to 2,867,541
pcm
b2744: surE - acid phosphatase (NCBI), at 2,867,535 to 2,868,296
surE
b2745: truD - tRNA pseudouridine synthase D (NCBI), at 2,868,277 to 2,869,326
truD
Position (kb)
2867
2868
2869 Strain fitness (log2 ratio)
-1
0
1 at 2866.658 kb on + strand, within nlpD at 2866.722 kb on - strand at 2866.836 kb on - strand at 2866.998 kb on + strand, within pcm at 2867.354 kb on + strand, within pcm at 2867.354 kb on + strand, within pcm at 2867.354 kb on + strand, within pcm at 2867.354 kb on + strand, within pcm at 2867.354 kb on + strand, within pcm at 2867.359 kb on - strand, within pcm at 2867.541 kb on - strand at 2867.541 kb on - strand at 2867.616 kb on - strand, within surE at 2867.623 kb on + strand, within surE at 2867.631 kb on + strand, within surE at 2867.672 kb on - strand, within surE at 2867.701 kb on + strand, within surE at 2867.709 kb on - strand, within surE at 2867.747 kb on + strand, within surE at 2867.747 kb on + strand, within surE at 2867.862 kb on - strand, within surE at 2867.862 kb on - strand, within surE at 2867.862 kb on - strand, within surE at 2867.883 kb on + strand, within surE at 2867.883 kb on + strand, within surE at 2868.038 kb on - strand, within surE at 2868.043 kb on - strand, within surE at 2868.043 kb on - strand, within surE at 2868.152 kb on + strand, within surE at 2868.266 kb on - strand at 2868.284 kb on + strand at 2868.332 kb on - strand at 2868.421 kb on - strand, within truD at 2868.647 kb on + strand, within truD at 2868.647 kb on - strand, within truD at 2868.744 kb on + strand, within truD at 2868.779 kb on + strand, within truD at 2868.779 kb on + strand, within truD at 2868.785 kb on - strand, within truD at 2868.785 kb on - strand, within truD at 2868.791 kb on + strand, within truD at 2868.845 kb on - strand, within truD at 2868.845 kb on - strand, within truD at 2868.930 kb on + strand, within truD at 2868.930 kb on + strand, within truD at 2868.986 kb on + strand, within truD at 2868.986 kb on + strand, within truD at 2868.996 kb on - strand, within truD at 2869.037 kb on + strand, within truD at 2869.118 kb on - strand, within truD at 2869.118 kb on - strand, within truD at 2869.194 kb on + strand, within truD at 2869.194 kb on + strand, within truD at 2869.292 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 3 remove 2,866,658 + nlpD b2742 0.90 -0.1 2,866,722 - +0.8 2,866,836 - -0.5 2,866,998 + pcm b2743 0.13 -0.7 2,867,354 + pcm b2743 0.70 -0.1 2,867,354 + pcm b2743 0.70 -1.2 2,867,354 + pcm b2743 0.70 +0.1 2,867,354 + pcm b2743 0.70 -0.5 2,867,354 + pcm b2743 0.70 +0.0 2,867,359 - pcm b2743 0.71 -1.3 2,867,541 - -0.0 2,867,541 - -0.1 2,867,616 - surE b2744 0.11 -1.7 2,867,623 + surE b2744 0.12 -0.4 2,867,631 + surE b2744 0.13 -1.3 2,867,672 - surE b2744 0.18 -1.1 2,867,701 + surE b2744 0.22 -0.2 2,867,709 - surE b2744 0.23 -0.4 2,867,747 + surE b2744 0.28 -0.7 2,867,747 + surE b2744 0.28 -0.5 2,867,862 - surE b2744 0.43 -0.7 2,867,862 - surE b2744 0.43 -0.9 2,867,862 - surE b2744 0.43 -0.6 2,867,883 + surE b2744 0.46 -0.4 2,867,883 + surE b2744 0.46 -0.6 2,868,038 - surE b2744 0.66 -1.0 2,868,043 - surE b2744 0.67 -0.2 2,868,043 - surE b2744 0.67 -0.3 2,868,152 + surE b2744 0.81 -1.0 2,868,266 - -1.1 2,868,284 + -1.0 2,868,332 - -0.4 2,868,421 - truD b2745 0.14 -0.2 2,868,647 + truD b2745 0.35 -0.5 2,868,647 - truD b2745 0.35 -0.4 2,868,744 + truD b2745 0.44 -0.6 2,868,779 + truD b2745 0.48 +0.0 2,868,779 + truD b2745 0.48 +0.5 2,868,785 - truD b2745 0.48 -0.4 2,868,785 - truD b2745 0.48 -0.1 2,868,791 + truD b2745 0.49 -0.4 2,868,845 - truD b2745 0.54 +0.6 2,868,845 - truD b2745 0.54 -0.4 2,868,930 + truD b2745 0.62 -0.3 2,868,930 + truD b2745 0.62 +0.2 2,868,986 + truD b2745 0.68 -1.0 2,868,986 + truD b2745 0.68 +0.3 2,868,996 - truD b2745 0.68 +0.1 2,869,037 + truD b2745 0.72 +1.3 2,869,118 - truD b2745 0.80 -1.0 2,869,118 - truD b2745 0.80 -0.4 2,869,194 + truD b2745 0.87 -0.5 2,869,194 + truD b2745 0.87 -0.8 2,869,292 - -0.4
Or see this region's nucleotide sequence