Experiment: Ying_Nucleobase14 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yacH and acnB are separated by 354 nucleotides acnB and yacL are separated by 175 nucleotides yacL and speD are separated by 37 nucleotides
b0117: yacH - hypothetical protein (NCBI), at 129,407 to 131,260
yacH
b0118: acnB - aconitate hydratase (NCBI), at 131,615 to 134,212
acnB
b0119: yacL - orf, hypothetical protein (VIMSS), at 134,388 to 134,750
yacL
b0120: speD - S-adenosylmethionine decarboxylase proenzyme (NCBI), at 134,788 to 135,582
speD
Position (kb) 131
132
133
134
135 Strain fitness (log2 ratio) -1
0
1
2
3 at 130.615 kb on + strand, within yacH at 130.615 kb on + strand, within yacH at 130.649 kb on - strand, within yacH at 130.649 kb on - strand, within yacH at 130.693 kb on + strand, within yacH at 130.732 kb on + strand, within yacH at 130.742 kb on + strand, within yacH at 130.742 kb on + strand, within yacH at 130.796 kb on + strand, within yacH at 130.871 kb on + strand, within yacH at 130.879 kb on - strand, within yacH at 130.930 kb on + strand, within yacH at 131.045 kb on - strand, within yacH at 131.228 kb on - strand at 131.228 kb on - strand at 131.369 kb on + strand at 131.369 kb on + strand at 131.389 kb on - strand at 131.518 kb on + strand at 132.875 kb on - strand, within acnB at 134.194 kb on + strand at 134.338 kb on + strand at 134.338 kb on + strand at 134.373 kb on + strand at 134.384 kb on + strand at 134.410 kb on + strand at 134.410 kb on + strand at 134.418 kb on - strand at 134.429 kb on + strand, within yacL at 134.430 kb on - strand, within yacL at 134.430 kb on - strand, within yacL at 134.432 kb on + strand, within yacL at 134.445 kb on + strand, within yacL at 134.445 kb on + strand, within yacL at 134.454 kb on - strand, within yacL at 134.454 kb on - strand, within yacL at 134.454 kb on - strand, within yacL at 134.456 kb on + strand, within yacL at 134.456 kb on + strand, within yacL at 134.549 kb on + strand, within yacL at 134.549 kb on + strand, within yacL at 134.553 kb on + strand, within yacL at 134.580 kb on + strand, within yacL at 134.598 kb on - strand, within yacL at 134.601 kb on - strand, within yacL at 134.657 kb on + strand, within yacL at 134.657 kb on + strand, within yacL at 134.666 kb on - strand, within yacL at 134.683 kb on + strand, within yacL at 134.690 kb on - strand, within yacL at 134.690 kb on - strand, within yacL at 134.831 kb on + strand at 134.878 kb on - strand, within speD at 134.878 kb on - strand, within speD at 134.899 kb on - strand, within speD at 134.899 kb on - strand, within speD at 134.900 kb on + strand, within speD at 134.900 kb on + strand, within speD at 134.903 kb on - strand, within speD at 134.980 kb on + strand, within speD at 134.980 kb on + strand, within speD at 135.008 kb on + strand, within speD at 135.018 kb on + strand, within speD at 135.048 kb on + strand, within speD at 135.048 kb on + strand, within speD
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 3 remove 130,615 + yacH b0117 0.65 +3.7 130,615 + yacH b0117 0.65 -0.7 130,649 - yacH b0117 0.67 -0.4 130,649 - yacH b0117 0.67 +0.1 130,693 + yacH b0117 0.69 +0.6 130,732 + yacH b0117 0.71 +0.7 130,742 + yacH b0117 0.72 +0.0 130,742 + yacH b0117 0.72 +0.2 130,796 + yacH b0117 0.75 -0.7 130,871 + yacH b0117 0.79 +3.4 130,879 - yacH b0117 0.79 +0.2 130,930 + yacH b0117 0.82 +0.0 131,045 - yacH b0117 0.88 -0.6 131,228 - +1.4 131,228 - -0.2 131,369 + +0.3 131,369 + -0.8 131,389 - +0.7 131,518 + -0.1 132,875 - acnB b0118 0.48 +1.5 134,194 + +0.2 134,338 + +0.3 134,338 + +0.3 134,373 + +0.4 134,384 + +0.0 134,410 + -0.5 134,410 + +0.2 134,418 - +0.4 134,429 + yacL b0119 0.11 -0.6 134,430 - yacL b0119 0.12 +0.7 134,430 - yacL b0119 0.12 -0.3 134,432 + yacL b0119 0.12 -0.1 134,445 + yacL b0119 0.16 -0.7 134,445 + yacL b0119 0.16 -0.0 134,454 - yacL b0119 0.18 -0.1 134,454 - yacL b0119 0.18 +0.1 134,454 - yacL b0119 0.18 -0.0 134,456 + yacL b0119 0.19 +0.8 134,456 + yacL b0119 0.19 -0.9 134,549 + yacL b0119 0.44 +0.3 134,549 + yacL b0119 0.44 +1.1 134,553 + yacL b0119 0.45 -0.8 134,580 + yacL b0119 0.53 -0.0 134,598 - yacL b0119 0.58 +0.1 134,601 - yacL b0119 0.59 -0.4 134,657 + yacL b0119 0.74 +1.0 134,657 + yacL b0119 0.74 +0.5 134,666 - yacL b0119 0.77 +0.5 134,683 + yacL b0119 0.81 -0.5 134,690 - yacL b0119 0.83 -0.1 134,690 - yacL b0119 0.83 +0.5 134,831 + -0.6 134,878 - speD b0120 0.11 -0.0 134,878 - speD b0120 0.11 -0.6 134,899 - speD b0120 0.14 +0.2 134,899 - speD b0120 0.14 +0.0 134,900 + speD b0120 0.14 +0.2 134,900 + speD b0120 0.14 +0.2 134,903 - speD b0120 0.14 +0.7 134,980 + speD b0120 0.24 -0.7 134,980 + speD b0120 0.24 -0.9 135,008 + speD b0120 0.28 -0.1 135,018 + speD b0120 0.29 -0.2 135,048 + speD b0120 0.33 -1.5 135,048 + speD b0120 0.33 -0.5
Or see this region's nucleotide sequence