Experiment: Ying_sugar7 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuO and ilvI are separated by 317 nucleotides ilvI and ilvH are separated by 2 nucleotides ilvH and fruR are separated by 179 nucleotides
b0076: leuO - probable transcriptional activator for leuABCD operon (VIMSS), at 84,368 to 85,312
leuO
b0077: ilvI - acetolactate synthase III large subunit (NCBI), at 85,630 to 87,354
ilvI
b0078: ilvH - acetolactate synthase small subunit (NCBI), at 87,357 to 87,848
ilvH
b0080: fruR - DNA-binding transcriptional dual regulator (NCBI), at 88,028 to 89,032
fruR
Position (kb)
85
86
87
88 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3 at 84.635 kb on + strand, within leuO at 84.635 kb on + strand, within leuO at 84.644 kb on - strand, within leuO at 84.648 kb on + strand, within leuO at 84.648 kb on + strand, within leuO at 84.648 kb on + strand, within leuO at 84.648 kb on + strand, within leuO at 84.658 kb on - strand, within leuO at 84.658 kb on - strand, within leuO at 84.671 kb on + strand, within leuO at 84.696 kb on + strand, within leuO at 84.696 kb on + strand, within leuO at 84.728 kb on + strand, within leuO at 84.728 kb on + strand, within leuO at 84.728 kb on + strand, within leuO at 84.728 kb on + strand, within leuO at 84.731 kb on + strand, within leuO at 84.736 kb on - strand, within leuO at 84.736 kb on - strand, within leuO at 84.769 kb on + strand, within leuO at 84.786 kb on + strand, within leuO at 84.786 kb on + strand, within leuO at 84.799 kb on + strand, within leuO at 84.799 kb on + strand, within leuO at 84.805 kb on - strand, within leuO at 84.805 kb on - strand, within leuO at 84.825 kb on + strand, within leuO at 84.825 kb on - strand, within leuO at 84.827 kb on + strand, within leuO at 84.827 kb on + strand, within leuO at 84.851 kb on - strand, within leuO at 84.859 kb on + strand, within leuO at 84.867 kb on + strand, within leuO at 84.900 kb on - strand, within leuO at 84.918 kb on + strand, within leuO at 84.918 kb on + strand, within leuO at 84.922 kb on - strand, within leuO at 84.947 kb on - strand, within leuO at 84.947 kb on - strand, within leuO at 84.975 kb on - strand, within leuO at 84.975 kb on - strand, within leuO at 84.978 kb on - strand, within leuO at 84.978 kb on - strand, within leuO at 84.990 kb on - strand, within leuO at 85.019 kb on - strand, within leuO at 85.028 kb on + strand, within leuO at 85.028 kb on + strand, within leuO at 85.037 kb on - strand, within leuO at 85.040 kb on + strand, within leuO at 85.045 kb on + strand, within leuO at 85.045 kb on + strand, within leuO at 85.048 kb on - strand, within leuO at 85.049 kb on + strand, within leuO at 85.050 kb on + strand, within leuO at 85.051 kb on - strand, within leuO at 85.051 kb on - strand, within leuO at 85.055 kb on + strand, within leuO at 85.055 kb on + strand, within leuO at 85.055 kb on + strand, within leuO at 85.058 kb on - strand, within leuO at 85.063 kb on - strand, within leuO at 85.063 kb on - strand, within leuO at 85.063 kb on - strand, within leuO at 85.064 kb on - strand, within leuO at 85.065 kb on - strand, within leuO at 85.066 kb on + strand, within leuO at 85.068 kb on + strand, within leuO at 85.069 kb on - strand, within leuO at 85.070 kb on + strand, within leuO at 85.071 kb on + strand, within leuO at 85.071 kb on + strand, within leuO at 85.071 kb on + strand, within leuO at 85.074 kb on - strand, within leuO at 85.076 kb on + strand, within leuO at 85.076 kb on - strand, within leuO at 85.076 kb on - strand, within leuO at 85.077 kb on + strand, within leuO at 85.079 kb on + strand, within leuO at 85.079 kb on + strand, within leuO at 85.079 kb on - strand, within leuO at 85.086 kb on + strand, within leuO at 85.086 kb on + strand, within leuO at 85.087 kb on - strand, within leuO at 85.087 kb on - strand, within leuO at 85.087 kb on - strand, within leuO at 85.098 kb on + strand, within leuO at 85.098 kb on + strand, within leuO at 85.098 kb on + strand, within leuO at 85.099 kb on - strand, within leuO at 85.099 kb on - strand, within leuO at 85.099 kb on - strand, within leuO at 85.102 kb on - strand, within leuO at 85.104 kb on + strand, within leuO at 85.104 kb on + strand, within leuO at 85.104 kb on + strand, within leuO at 85.104 kb on + strand, within leuO at 85.104 kb on + strand, within leuO at 85.104 kb on + strand, within leuO at 85.104 kb on + strand, within leuO at 85.105 kb on + strand, within leuO at 85.105 kb on + strand, within leuO at 85.108 kb on - strand, within leuO at 85.108 kb on - strand, within leuO at 85.108 kb on - strand, within leuO at 85.108 kb on - strand, within leuO at 85.110 kb on + strand, within leuO at 85.112 kb on - strand, within leuO at 85.112 kb on - strand, within leuO at 85.112 kb on - strand, within leuO at 85.137 kb on + strand, within leuO at 85.137 kb on + strand, within leuO at 85.137 kb on + strand, within leuO at 85.137 kb on + strand, within leuO at 85.145 kb on - strand, within leuO at 85.145 kb on - strand, within leuO at 85.147 kb on + strand, within leuO at 85.157 kb on + strand, within leuO at 85.157 kb on + strand, within leuO at 85.157 kb on + strand, within leuO at 85.157 kb on + strand, within leuO at 85.216 kb on - strand, within leuO at 85.224 kb on + strand at 85.224 kb on + strand at 85.224 kb on - strand at 85.227 kb on + strand at 85.227 kb on + strand at 85.234 kb on + strand at 85.234 kb on + strand at 85.234 kb on + strand at 85.234 kb on + strand at 85.240 kb on + strand at 85.250 kb on + strand at 85.250 kb on + strand at 85.259 kb on + strand at 85.266 kb on - strand at 85.273 kb on + strand at 85.277 kb on + strand at 85.292 kb on + strand at 85.327 kb on + strand at 85.327 kb on + strand at 85.327 kb on + strand at 85.332 kb on + strand at 85.332 kb on + strand at 85.334 kb on + strand at 85.388 kb on + strand at 85.388 kb on + strand at 85.548 kb on - strand at 85.548 kb on - strand at 85.582 kb on - strand at 85.582 kb on - strand at 85.584 kb on + strand at 85.592 kb on - strand at 85.620 kb on + strand at 85.623 kb on - strand at 85.626 kb on + strand at 85.626 kb on + strand at 85.626 kb on + strand at 85.626 kb on + strand at 85.626 kb on - strand at 85.627 kb on + strand at 85.627 kb on + strand at 85.634 kb on - strand at 85.634 kb on - strand at 85.634 kb on - strand at 85.634 kb on - strand at 85.635 kb on - strand at 85.635 kb on - strand at 85.635 kb on - strand at 85.635 kb on - strand at 85.636 kb on - strand at 85.638 kb on - strand at 85.643 kb on - strand at 85.643 kb on - strand at 85.646 kb on - strand at 85.646 kb on - strand at 85.649 kb on - strand at 85.649 kb on - strand at 85.725 kb on - strand at 85.725 kb on - strand at 85.738 kb on + strand at 85.738 kb on + strand at 85.745 kb on - strand at 85.745 kb on - strand at 85.757 kb on - strand at 85.765 kb on + strand at 85.793 kb on - strand at 85.794 kb on - strand at 85.802 kb on - strand at 85.802 kb on - strand at 85.802 kb on - strand at 85.802 kb on - strand at 85.815 kb on - strand, within ilvI at 85.815 kb on - strand, within ilvI at 85.830 kb on + strand, within ilvI at 85.856 kb on + strand, within ilvI at 85.856 kb on + strand, within ilvI at 85.864 kb on - strand, within ilvI at 85.931 kb on + strand, within ilvI at 85.937 kb on + strand, within ilvI at 86.112 kb on + strand, within ilvI at 86.348 kb on + strand, within ilvI at 86.348 kb on + strand, within ilvI at 86.357 kb on + strand, within ilvI at 86.357 kb on + strand, within ilvI at 86.366 kb on - strand, within ilvI at 86.367 kb on + strand, within ilvI at 86.438 kb on + strand, within ilvI at 86.447 kb on - strand, within ilvI at 86.447 kb on - strand, within ilvI at 86.508 kb on + strand, within ilvI at 86.517 kb on + strand, within ilvI at 86.535 kb on - strand, within ilvI at 86.535 kb on - strand, within ilvI at 86.548 kb on - strand, within ilvI at 86.593 kb on - strand, within ilvI at 86.602 kb on + strand, within ilvI at 86.602 kb on + strand, within ilvI at 86.797 kb on + strand, within ilvI at 86.841 kb on + strand, within ilvI at 86.894 kb on - strand, within ilvI at 86.899 kb on + strand, within ilvI at 87.001 kb on - strand, within ilvI at 87.026 kb on + strand, within ilvI at 87.034 kb on - strand, within ilvI at 87.062 kb on - strand, within ilvI at 87.070 kb on + strand, within ilvI at 87.070 kb on + strand, within ilvI at 87.092 kb on - strand, within ilvI at 87.098 kb on - strand, within ilvI at 87.098 kb on - strand, within ilvI at 87.130 kb on + strand, within ilvI at 87.165 kb on - strand, within ilvI at 87.166 kb on - strand, within ilvI at 87.250 kb on + strand at 87.285 kb on + strand at 87.443 kb on - strand, within ilvH at 87.480 kb on + strand, within ilvH at 87.497 kb on + strand, within ilvH at 87.571 kb on + strand, within ilvH at 87.571 kb on + strand, within ilvH at 87.588 kb on - strand, within ilvH at 87.617 kb on - strand, within ilvH at 87.617 kb on - strand, within ilvH at 87.640 kb on + strand, within ilvH at 87.665 kb on - strand, within ilvH at 87.698 kb on - strand, within ilvH at 87.705 kb on + strand, within ilvH at 87.710 kb on + strand, within ilvH at 87.710 kb on + strand, within ilvH at 87.718 kb on - strand, within ilvH at 87.718 kb on - strand, within ilvH at 87.718 kb on - strand, within ilvH at 87.718 kb on - strand, within ilvH at 87.740 kb on - strand, within ilvH at 87.744 kb on - strand, within ilvH at 87.785 kb on + strand, within ilvH at 87.802 kb on + strand at 87.815 kb on - strand at 87.918 kb on - strand at 87.951 kb on - strand at 87.955 kb on + strand at 88.009 kb on + strand at 88.074 kb on + strand at 88.085 kb on + strand at 88.111 kb on - strand at 88.111 kb on - strand at 88.111 kb on - strand at 88.111 kb on - strand at 88.113 kb on - strand at 88.187 kb on + strand, within fruR at 88.187 kb on + strand, within fruR at 88.259 kb on - strand, within fruR at 88.265 kb on + strand, within fruR
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 3 remove 84,635 + leuO b0076 0.28 -0.7 84,635 + leuO b0076 0.28 -0.1 84,644 - leuO b0076 0.29 +2.8 84,648 + leuO b0076 0.30 +0.1 84,648 + leuO b0076 0.30 +0.4 84,648 + leuO b0076 0.30 +0.6 84,648 + leuO b0076 0.30 +0.5 84,658 - leuO b0076 0.31 +0.8 84,658 - leuO b0076 0.31 +0.2 84,671 + leuO b0076 0.32 -1.4 84,696 + leuO b0076 0.35 +0.3 84,696 + leuO b0076 0.35 +0.4 84,728 + leuO b0076 0.38 +0.6 84,728 + leuO b0076 0.38 +0.2 84,728 + leuO b0076 0.38 +1.6 84,728 + leuO b0076 0.38 -0.7 84,731 + leuO b0076 0.38 +0.3 84,736 - leuO b0076 0.39 +1.8 84,736 - leuO b0076 0.39 -1.4 84,769 + leuO b0076 0.42 -1.1 84,786 + leuO b0076 0.44 +0.1 84,786 + leuO b0076 0.44 +0.1 84,799 + leuO b0076 0.46 -0.5 84,799 + leuO b0076 0.46 +0.1 84,805 - leuO b0076 0.46 +0.5 84,805 - leuO b0076 0.46 +1.6 84,825 + leuO b0076 0.48 -1.1 84,825 - leuO b0076 0.48 -0.4 84,827 + leuO b0076 0.49 -1.4 84,827 + leuO b0076 0.49 +0.3 84,851 - leuO b0076 0.51 -0.6 84,859 + leuO b0076 0.52 +0.1 84,867 + leuO b0076 0.53 -2.0 84,900 - leuO b0076 0.56 +2.4 84,918 + leuO b0076 0.58 +0.1 84,918 + leuO b0076 0.58 -0.6 84,922 - leuO b0076 0.59 +0.8 84,947 - leuO b0076 0.61 +0.3 84,947 - leuO b0076 0.61 -0.9 84,975 - leuO b0076 0.64 +0.5 84,975 - leuO b0076 0.64 -0.3 84,978 - leuO b0076 0.65 -1.6 84,978 - leuO b0076 0.65 -1.6 84,990 - leuO b0076 0.66 -0.1 85,019 - leuO b0076 0.69 +0.8 85,028 + leuO b0076 0.70 -0.8 85,028 + leuO b0076 0.70 -0.9 85,037 - leuO b0076 0.71 -1.0 85,040 + leuO b0076 0.71 -0.0 85,045 + leuO b0076 0.72 -0.3 85,045 + leuO b0076 0.72 -1.3 85,048 - leuO b0076 0.72 -0.3 85,049 + leuO b0076 0.72 -0.1 85,050 + leuO b0076 0.72 -0.1 85,051 - leuO b0076 0.72 -0.9 85,051 - leuO b0076 0.72 -0.4 85,055 + leuO b0076 0.73 -1.9 85,055 + leuO b0076 0.73 -1.4 85,055 + leuO b0076 0.73 -1.2 85,058 - leuO b0076 0.73 +1.3 85,063 - leuO b0076 0.74 +1.8 85,063 - leuO b0076 0.74 +0.8 85,063 - leuO b0076 0.74 -0.5 85,064 - leuO b0076 0.74 +0.2 85,065 - leuO b0076 0.74 +0.6 85,066 + leuO b0076 0.74 -1.5 85,068 + leuO b0076 0.74 +0.7 85,069 - leuO b0076 0.74 +0.5 85,070 + leuO b0076 0.74 -0.1 85,071 + leuO b0076 0.74 -0.4 85,071 + leuO b0076 0.74 +0.4 85,071 + leuO b0076 0.74 +1.4 85,074 - leuO b0076 0.75 +0.6 85,076 + leuO b0076 0.75 +0.1 85,076 - leuO b0076 0.75 +0.2 85,076 - leuO b0076 0.75 +0.7 85,077 + leuO b0076 0.75 -0.9 85,079 + leuO b0076 0.75 -0.1 85,079 + leuO b0076 0.75 -0.1 85,079 - leuO b0076 0.75 -0.7 85,086 + leuO b0076 0.76 -0.0 85,086 + leuO b0076 0.76 -0.0 85,087 - leuO b0076 0.76 +0.7 85,087 - leuO b0076 0.76 +1.9 85,087 - leuO b0076 0.76 +1.2 85,098 + leuO b0076 0.77 -0.5 85,098 + leuO b0076 0.77 -0.4 85,098 + leuO b0076 0.77 -0.5 85,099 - leuO b0076 0.77 +1.2 85,099 - leuO b0076 0.77 -0.3 85,099 - leuO b0076 0.77 -0.6 85,102 - leuO b0076 0.78 +0.7 85,104 + leuO b0076 0.78 +0.3 85,104 + leuO b0076 0.78 +0.5 85,104 + leuO b0076 0.78 +0.3 85,104 + leuO b0076 0.78 -0.1 85,104 + leuO b0076 0.78 +0.4 85,104 + leuO b0076 0.78 +0.1 85,104 + leuO b0076 0.78 +0.2 85,105 + leuO b0076 0.78 -0.9 85,105 + leuO b0076 0.78 -0.3 85,108 - leuO b0076 0.78 -0.4 85,108 - leuO b0076 0.78 -0.2 85,108 - leuO b0076 0.78 +1.6 85,108 - leuO b0076 0.78 +0.4 85,110 + leuO b0076 0.79 +0.4 85,112 - leuO b0076 0.79 -0.5 85,112 - leuO b0076 0.79 -1.0 85,112 - leuO b0076 0.79 -2.0 85,137 + leuO b0076 0.81 -0.3 85,137 + leuO b0076 0.81 +1.4 85,137 + leuO b0076 0.81 -1.0 85,137 + leuO b0076 0.81 -0.8 85,145 - leuO b0076 0.82 +1.8 85,145 - leuO b0076 0.82 +1.8 85,147 + leuO b0076 0.82 +0.1 85,157 + leuO b0076 0.83 -1.6 85,157 + leuO b0076 0.83 -0.1 85,157 + leuO b0076 0.83 -0.3 85,157 + leuO b0076 0.83 -0.9 85,216 - leuO b0076 0.90 +2.8 85,224 + +0.3 85,224 + -0.0 85,224 - -1.2 85,227 + -0.7 85,227 + -1.4 85,234 + +0.6 85,234 + +0.3 85,234 + +0.5 85,234 + -0.6 85,240 + -0.2 85,250 + -0.6 85,250 + -0.5 85,259 + -0.7 85,266 - +0.5 85,273 + -1.8 85,277 + +0.2 85,292 + -1.1 85,327 + -0.8 85,327 + -0.0 85,327 + -0.3 85,332 + +0.0 85,332 + -0.8 85,334 + -0.3 85,388 + -2.4 85,388 + -2.1 85,548 - -2.5 85,548 - -4.4 85,582 - -3.6 85,582 - -3.6 85,584 + +0.4 85,592 - -4.5 85,620 + +0.3 85,623 - -3.6 85,626 + -0.7 85,626 + +0.7 85,626 + +1.1 85,626 + -0.5 85,626 - -5.2 85,627 + -0.9 85,627 + -2.2 85,634 - -2.5 85,634 - -2.6 85,634 - -3.1 85,634 - -1.9 85,635 - -5.1 85,635 - -2.2 85,635 - -3.2 85,635 - -3.9 85,636 - -4.5 85,638 - -3.6 85,643 - -5.1 85,643 - -4.2 85,646 - -3.3 85,646 - -2.7 85,649 - -2.7 85,649 - -4.3 85,725 - -0.2 85,725 - -4.1 85,738 + -4.3 85,738 + -3.0 85,745 - -4.4 85,745 - -1.0 85,757 - -2.5 85,765 + -2.9 85,793 - -4.8 85,794 - -3.5 85,802 - -3.7 85,802 - -2.2 85,802 - -1.6 85,802 - -1.7 85,815 - ilvI b0077 0.11 -2.9 85,815 - ilvI b0077 0.11 -3.7 85,830 + ilvI b0077 0.12 -3.8 85,856 + ilvI b0077 0.13 -3.7 85,856 + ilvI b0077 0.13 -1.8 85,864 - ilvI b0077 0.14 -2.6 85,931 + ilvI b0077 0.17 -3.7 85,937 + ilvI b0077 0.18 -4.9 86,112 + ilvI b0077 0.28 -4.2 86,348 + ilvI b0077 0.42 -2.4 86,348 + ilvI b0077 0.42 -1.5 86,357 + ilvI b0077 0.42 -3.6 86,357 + ilvI b0077 0.42 -3.0 86,366 - ilvI b0077 0.43 -3.6 86,367 + ilvI b0077 0.43 -5.4 86,438 + ilvI b0077 0.47 -1.5 86,447 - ilvI b0077 0.47 -3.5 86,447 - ilvI b0077 0.47 -3.9 86,508 + ilvI b0077 0.51 -4.1 86,517 + ilvI b0077 0.51 -4.5 86,535 - ilvI b0077 0.52 -4.4 86,535 - ilvI b0077 0.52 -4.5 86,548 - ilvI b0077 0.53 -5.4 86,593 - ilvI b0077 0.56 -4.3 86,602 + ilvI b0077 0.56 -2.8 86,602 + ilvI b0077 0.56 -4.0 86,797 + ilvI b0077 0.68 -2.2 86,841 + ilvI b0077 0.70 -3.9 86,894 - ilvI b0077 0.73 -3.0 86,899 + ilvI b0077 0.74 -4.3 87,001 - ilvI b0077 0.79 -4.3 87,026 + ilvI b0077 0.81 -3.0 87,034 - ilvI b0077 0.81 -5.0 87,062 - ilvI b0077 0.83 -3.1 87,070 + ilvI b0077 0.83 -4.8 87,070 + ilvI b0077 0.83 -5.3 87,092 - ilvI b0077 0.85 -5.0 87,098 - ilvI b0077 0.85 -3.3 87,098 - ilvI b0077 0.85 -3.5 87,130 + ilvI b0077 0.87 -4.9 87,165 - ilvI b0077 0.89 -5.5 87,166 - ilvI b0077 0.89 -4.2 87,250 + -2.6 87,285 + -3.0 87,443 - ilvH b0078 0.17 -1.2 87,480 + ilvH b0078 0.25 -4.4 87,497 + ilvH b0078 0.28 -4.5 87,571 + ilvH b0078 0.43 -4.1 87,571 + ilvH b0078 0.43 -3.7 87,588 - ilvH b0078 0.47 -3.8 87,617 - ilvH b0078 0.53 -1.9 87,617 - ilvH b0078 0.53 -2.0 87,640 + ilvH b0078 0.58 -3.1 87,665 - ilvH b0078 0.63 -1.9 87,698 - ilvH b0078 0.69 -1.3 87,705 + ilvH b0078 0.71 -3.2 87,710 + ilvH b0078 0.72 -4.0 87,710 + ilvH b0078 0.72 -3.3 87,718 - ilvH b0078 0.73 -4.4 87,718 - ilvH b0078 0.73 -4.0 87,718 - ilvH b0078 0.73 -3.4 87,718 - ilvH b0078 0.73 -3.5 87,740 - ilvH b0078 0.78 -6.3 87,744 - ilvH b0078 0.79 -3.8 87,785 + ilvH b0078 0.87 -3.4 87,802 + -1.1 87,815 - -1.6 87,918 - -0.4 87,951 - -1.3 87,955 + -0.1 88,009 + +0.4 88,074 + -0.9 88,085 + -1.2 88,111 - -1.6 88,111 - -1.4 88,111 - -1.9 88,111 - -2.8 88,113 - -1.9 88,187 + fruR b0080 0.16 -1.2 88,187 + fruR b0080 0.16 -1.2 88,259 - fruR b0080 0.23 -1.7 88,265 + fruR b0080 0.24 -1.4
Or see this region's nucleotide sequence