Experiment: Ying_sugar7 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt emrA and emrB are separated by 16 nucleotides emrB and luxS are separated by 63 nucleotides luxS and micA are separated by 68 nucleotides micA and gshA are separated by 3 nucleotides
b2685: emrA - multidrug efflux system (NCBI), at 2,809,449 to 2,810,621
emrA
b2686: emrB - multidrug efflux system protein (NCBI), at 2,810,638 to 2,812,176
emrB
b2687: luxS - S-ribosylhomocysteinase (NCBI), at 2,812,240 to 2,812,755
luxS
b4442: micA - sRNA effector of ompA mRNA instability in stationary phase; requires Hfq (RefSeq), at 2,812,824 to 2,812,901
micA
b2688: gshA - glutamate--cysteine ligase (NCBI), at 2,812,905 to 2,814,461
gshA
Position (kb)
2810
2811
2812
2813 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2809.748 kb on - strand, within emrA at 2809.748 kb on - strand, within emrA at 2809.806 kb on - strand, within emrA at 2809.931 kb on - strand, within emrA at 2810.041 kb on + strand, within emrA at 2810.097 kb on + strand, within emrA at 2810.097 kb on + strand, within emrA at 2810.097 kb on + strand, within emrA at 2810.100 kb on - strand, within emrA at 2810.100 kb on - strand, within emrA at 2810.103 kb on - strand, within emrA at 2810.127 kb on - strand, within emrA at 2810.147 kb on - strand, within emrA at 2810.204 kb on - strand, within emrA at 2810.320 kb on + strand, within emrA at 2810.339 kb on - strand, within emrA at 2810.417 kb on - strand, within emrA at 2810.443 kb on - strand, within emrA at 2810.480 kb on + strand, within emrA at 2810.482 kb on - strand, within emrA at 2810.482 kb on - strand, within emrA at 2810.503 kb on + strand, within emrA at 2810.533 kb on + strand at 2810.543 kb on + strand at 2810.543 kb on + strand at 2810.549 kb on - strand at 2810.621 kb on + strand at 2810.633 kb on + strand at 2810.716 kb on + strand at 2810.716 kb on + strand at 2810.724 kb on - strand at 2810.763 kb on - strand at 2810.764 kb on + strand at 2810.764 kb on + strand at 2810.921 kb on + strand, within emrB at 2811.019 kb on - strand, within emrB at 2811.019 kb on - strand, within emrB at 2811.034 kb on + strand, within emrB at 2811.034 kb on + strand, within emrB at 2811.151 kb on - strand, within emrB at 2811.196 kb on - strand, within emrB at 2811.196 kb on - strand, within emrB at 2811.310 kb on + strand, within emrB at 2811.431 kb on - strand, within emrB at 2811.436 kb on - strand, within emrB at 2811.505 kb on - strand, within emrB at 2811.507 kb on - strand, within emrB at 2811.507 kb on - strand, within emrB at 2811.528 kb on + strand at 2811.581 kb on - strand, within emrB at 2811.778 kb on - strand, within emrB at 2811.788 kb on + strand, within emrB at 2811.788 kb on + strand, within emrB at 2811.796 kb on - strand, within emrB at 2811.889 kb on - strand, within emrB at 2811.906 kb on - strand, within emrB at 2811.983 kb on + strand, within emrB at 2811.983 kb on + strand, within emrB at 2812.033 kb on - strand at 2812.033 kb on - strand at 2812.113 kb on - strand at 2812.113 kb on - strand at 2812.127 kb on + strand at 2812.150 kb on + strand at 2812.150 kb on + strand at 2812.178 kb on - strand at 2812.327 kb on + strand, within luxS at 2812.334 kb on - strand, within luxS at 2812.375 kb on - strand, within luxS at 2812.383 kb on - strand, within luxS at 2812.383 kb on - strand, within luxS at 2812.467 kb on + strand, within luxS at 2812.470 kb on - strand, within luxS at 2812.525 kb on + strand, within luxS at 2812.525 kb on + strand, within luxS at 2812.534 kb on - strand, within luxS at 2812.541 kb on - strand, within luxS at 2812.547 kb on - strand, within luxS at 2812.585 kb on + strand, within luxS at 2812.604 kb on - strand, within luxS at 2812.604 kb on - strand, within luxS at 2812.637 kb on - strand, within luxS at 2812.719 kb on + strand at 2812.819 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 3 remove 2,809,748 - emrA b2685 0.25 -0.5 2,809,748 - emrA b2685 0.25 +0.4 2,809,806 - emrA b2685 0.30 -2.0 2,809,931 - emrA b2685 0.41 -2.5 2,810,041 + emrA b2685 0.50 -0.5 2,810,097 + emrA b2685 0.55 -1.2 2,810,097 + emrA b2685 0.55 -1.3 2,810,097 + emrA b2685 0.55 -2.4 2,810,100 - emrA b2685 0.55 -2.0 2,810,100 - emrA b2685 0.55 -4.0 2,810,103 - emrA b2685 0.56 -2.2 2,810,127 - emrA b2685 0.58 -1.8 2,810,147 - emrA b2685 0.60 -0.9 2,810,204 - emrA b2685 0.64 -1.2 2,810,320 + emrA b2685 0.74 -1.0 2,810,339 - emrA b2685 0.76 -2.6 2,810,417 - emrA b2685 0.83 -2.9 2,810,443 - emrA b2685 0.85 -1.5 2,810,480 + emrA b2685 0.88 -2.1 2,810,482 - emrA b2685 0.88 -1.8 2,810,482 - emrA b2685 0.88 -1.8 2,810,503 + emrA b2685 0.90 -2.0 2,810,533 + -2.5 2,810,543 + -1.5 2,810,543 + -1.0 2,810,549 - -2.3 2,810,621 + -0.1 2,810,633 + +0.1 2,810,716 + -1.3 2,810,716 + -1.1 2,810,724 - -0.9 2,810,763 - -0.7 2,810,764 + -1.8 2,810,764 + -1.4 2,810,921 + emrB b2686 0.18 -1.2 2,811,019 - emrB b2686 0.25 -1.7 2,811,019 - emrB b2686 0.25 -3.0 2,811,034 + emrB b2686 0.26 -0.2 2,811,034 + emrB b2686 0.26 -1.4 2,811,151 - emrB b2686 0.33 -0.8 2,811,196 - emrB b2686 0.36 -2.1 2,811,196 - emrB b2686 0.36 -0.2 2,811,310 + emrB b2686 0.44 -1.1 2,811,431 - emrB b2686 0.52 -1.6 2,811,436 - emrB b2686 0.52 -1.5 2,811,505 - emrB b2686 0.56 -1.4 2,811,507 - emrB b2686 0.56 -2.3 2,811,507 - emrB b2686 0.56 -3.2 2,811,528 + -0.7 2,811,581 - emrB b2686 0.61 -0.6 2,811,778 - emrB b2686 0.74 -2.7 2,811,788 + emrB b2686 0.75 -2.9 2,811,788 + emrB b2686 0.75 -1.2 2,811,796 - emrB b2686 0.75 -0.8 2,811,889 - emrB b2686 0.81 -1.2 2,811,906 - emrB b2686 0.82 -1.7 2,811,983 + emrB b2686 0.87 -1.2 2,811,983 + emrB b2686 0.87 -2.6 2,812,033 - -3.0 2,812,033 - -1.5 2,812,113 - -1.1 2,812,113 - -0.5 2,812,127 + -1.4 2,812,150 + -0.9 2,812,150 + +0.1 2,812,178 - -1.0 2,812,327 + luxS b2687 0.17 +0.2 2,812,334 - luxS b2687 0.18 -0.2 2,812,375 - luxS b2687 0.26 -0.1 2,812,383 - luxS b2687 0.28 -0.6 2,812,383 - luxS b2687 0.28 +0.2 2,812,467 + luxS b2687 0.44 +0.9 2,812,470 - luxS b2687 0.45 -1.0 2,812,525 + luxS b2687 0.55 +1.0 2,812,525 + luxS b2687 0.55 -0.0 2,812,534 - luxS b2687 0.57 +1.0 2,812,541 - luxS b2687 0.58 -0.6 2,812,547 - luxS b2687 0.59 -1.2 2,812,585 + luxS b2687 0.67 +0.8 2,812,604 - luxS b2687 0.71 -0.8 2,812,604 - luxS b2687 0.71 -0.5 2,812,637 - luxS b2687 0.77 -0.3 2,812,719 + +0.9 2,812,819 - -0.5
Or see this region's nucleotide sequence