Strain Fitness in Escherichia coli BW25113 around b2425

Experiment: Ying_sugar7 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcysW and cysU overlap by 1 nucleotidescysU and cysP overlap by 1 nucleotidescysP and ucpA are separated by 303 nucleotides b2423: cysW - sulfate/thiosulfate transporter subunit (NCBI), at 2,538,826 to 2,539,701 cysW b2424: cysU - sulfate/thiosulfate transporter subunit (NCBI), at 2,539,701 to 2,540,534 cysU b2425: cysP - thiosulfate transporter subunit (NCBI), at 2,540,534 to 2,541,550 cysP b2426: ucpA - putative oxidoreductase (VIMSS), at 2,541,854 to 2,542,645 ucpA Position (kb) 2540 2541 2542Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 2539.694 kb on - strandat 2540.062 kb on + strand, within cysUat 2540.110 kb on - strand, within cysUat 2540.110 kb on - strand, within cysUat 2540.129 kb on + strand, within cysUat 2540.492 kb on + strandat 2540.535 kb on + strandat 2540.563 kb on - strandat 2540.677 kb on - strand, within cysPat 2540.677 kb on - strand, within cysPat 2540.695 kb on - strand, within cysPat 2540.752 kb on - strand, within cysPat 2540.816 kb on - strand, within cysPat 2540.858 kb on + strand, within cysPat 2540.872 kb on + strand, within cysPat 2541.064 kb on + strand, within cysPat 2541.066 kb on + strand, within cysPat 2541.077 kb on - strand, within cysPat 2541.159 kb on + strand, within cysPat 2541.181 kb on - strand, within cysPat 2541.194 kb on + strand, within cysPat 2541.229 kb on - strand, within cysPat 2541.421 kb on - strand, within cysPat 2541.621 kb on + strandat 2541.699 kb on + strandat 2541.733 kb on + strandat 2542.113 kb on + strand, within ucpAat 2542.215 kb on - strand, within ucpAat 2542.432 kb on - strand, within ucpA

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 3
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2,539,694 - -4.1
2,540,062 + cysU b2424 0.43 -3.6
2,540,110 - cysU b2424 0.49 -4.9
2,540,110 - cysU b2424 0.49 -3.9
2,540,129 + cysU b2424 0.51 -4.1
2,540,492 + -3.1
2,540,535 + -4.0
2,540,563 - -2.4
2,540,677 - cysP b2425 0.14 +0.3
2,540,677 - cysP b2425 0.14 -0.4
2,540,695 - cysP b2425 0.16 -2.1
2,540,752 - cysP b2425 0.21 -4.0
2,540,816 - cysP b2425 0.28 +0.2
2,540,858 + cysP b2425 0.32 -4.0
2,540,872 + cysP b2425 0.33 -4.8
2,541,064 + cysP b2425 0.52 -5.4
2,541,066 + cysP b2425 0.52 -3.2
2,541,077 - cysP b2425 0.53 +0.2
2,541,159 + cysP b2425 0.61 -2.9
2,541,181 - cysP b2425 0.64 -0.7
2,541,194 + cysP b2425 0.65 -5.6
2,541,229 - cysP b2425 0.68 -1.1
2,541,421 - cysP b2425 0.87 -0.1
2,541,621 + -2.4
2,541,699 + -0.1
2,541,733 + -0.3
2,542,113 + ucpA b2426 0.33 +0.0
2,542,215 - ucpA b2426 0.46 -0.0
2,542,432 - ucpA b2426 0.73 +0.1

Or see this region's nucleotide sequence