Strain Fitness in Escherichia coli BW25113 around b1446

Experiment: Ying_sugar7 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydcW and yncL are separated by 96 nucleotidesyncL and b1445 are separated by 194 nucleotidesb1445 and ydcY are separated by 85 nucleotidesydcY and ydcZ are separated by 0 nucleotidesydcZ and yncA overlap by 4 nucleotides b1444: ydcW - medium chain aldehyde dehydrogenase (NCBI), at 1,513,602 to 1,515,026 ydcW b4598: yncL - hypothetical protein (RefSeq), at 1,515,123 to 1,515,218 yncL b1445: b1445 - orf, hypothetical protein (VIMSS), at 1,515,413 to 1,515,586 b1445 b1446: ydcY - hypothetical protein (NCBI), at 1,515,672 to 1,515,905 ydcY b1447: ydcZ - predicted inner membrane protein (NCBI), at 1,515,906 to 1,516,355 ydcZ b1448: yncA - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 1,516,352 to 1,516,870 yncA Position (kb) 1515 1516Strain fitness (log2 ratio) -1 0 1at 1514.706 kb on + strand, within ydcWat 1514.920 kb on + strandat 1515.090 kb on + strandat 1515.199 kb on - strand, within yncLat 1515.247 kb on + strandat 1515.249 kb on + strandat 1515.252 kb on - strandat 1515.257 kb on - strandat 1515.257 kb on - strandat 1515.259 kb on + strandat 1515.608 kb on + strandat 1515.670 kb on - strandat 1515.791 kb on + strand, within ydcYat 1515.839 kb on - strand, within ydcYat 1515.839 kb on - strand, within ydcYat 1515.892 kb on + strandat 1515.892 kb on + strandat 1516.613 kb on - strand, within yncAat 1516.789 kb on - strand, within yncAat 1516.831 kb on + strandat 1516.870 kb on + strandat 1516.878 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 3
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1,514,706 + ydcW b1444 0.77 -0.5
1,514,920 + -0.2
1,515,090 + -0.6
1,515,199 - yncL b4598 0.79 -0.3
1,515,247 + +0.5
1,515,249 + -0.2
1,515,252 - -0.3
1,515,257 - +0.0
1,515,257 - -0.1
1,515,259 + +0.2
1,515,608 + -1.0
1,515,670 - -0.2
1,515,791 + ydcY b1446 0.51 +0.8
1,515,839 - ydcY b1446 0.71 +0.7
1,515,839 - ydcY b1446 0.71 +0.4
1,515,892 + -0.3
1,515,892 + -0.3
1,516,613 - yncA b1448 0.50 -0.4
1,516,789 - yncA b1448 0.84 +0.1
1,516,831 + -0.4
1,516,870 + -0.1
1,516,878 - -0.6

Or see this region's nucleotide sequence