Experiment: Ying_sugar7 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt macB and cspD are separated by 72 nucleotides cspD and clpS are separated by 322 nucleotides clpS and clpA are separated by 30 nucleotides clpA and serW are separated by 343 nucleotides serW and infA are separated by 253 nucleotides
b0879: macB - fused macrolide transporter subunits of ABC superfamily: ATP-binding component/membrane component (NCBI), at 919,570 to 921,516
macB
b0880: cspD - cold shock protein homolog (NCBI), at 921,589 to 921,813
cspD
b0881: clpS - ATP-dependent Clp protease adaptor protein ClpS (NCBI), at 922,136 to 922,456
clpS
b0882: clpA - ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity (NCBI), at 922,487 to 924,763
clpA
b0883: serW - tRNA-Ser (NCBI), at 925,107 to 925,194
serW
b0884: infA - translation initiation factor IF-1 (NCBI), at 925,448 to 925,666
infA
Position (kb)
922
923
924
925 Strain fitness (log2 ratio)
-1
0
1
2 at 921.790 kb on + strand, within cspD at 921.790 kb on + strand, within cspD at 921.956 kb on - strand at 921.956 kb on - strand at 922.042 kb on + strand at 922.042 kb on + strand at 922.055 kb on + strand at 922.069 kb on + strand at 922.091 kb on - strand at 922.103 kb on + strand at 922.124 kb on + strand at 922.246 kb on - strand, within clpS at 922.417 kb on + strand, within clpS at 922.435 kb on - strand at 922.562 kb on + strand at 922.686 kb on - strand at 922.695 kb on - strand at 922.703 kb on + strand at 922.703 kb on + strand at 922.745 kb on + strand, within clpA at 922.745 kb on + strand, within clpA at 922.767 kb on + strand, within clpA at 922.767 kb on + strand, within clpA at 922.850 kb on + strand, within clpA at 922.877 kb on - strand, within clpA at 922.904 kb on - strand, within clpA at 923.228 kb on + strand, within clpA at 923.236 kb on - strand, within clpA at 923.381 kb on + strand, within clpA at 923.462 kb on - strand, within clpA at 923.523 kb on + strand, within clpA at 923.523 kb on + strand, within clpA at 923.595 kb on - strand, within clpA at 923.595 kb on - strand, within clpA at 923.644 kb on + strand, within clpA at 923.666 kb on - strand, within clpA at 923.736 kb on - strand, within clpA at 923.793 kb on + strand, within clpA at 923.903 kb on + strand, within clpA at 923.903 kb on + strand, within clpA at 923.924 kb on - strand, within clpA at 923.982 kb on - strand, within clpA at 923.999 kb on + strand, within clpA at 924.056 kb on - strand, within clpA at 924.056 kb on - strand, within clpA at 924.082 kb on + strand, within clpA at 924.093 kb on - strand, within clpA at 924.151 kb on - strand, within clpA at 924.151 kb on - strand, within clpA at 924.151 kb on - strand, within clpA at 924.344 kb on + strand, within clpA at 924.344 kb on + strand, within clpA at 924.542 kb on + strand at 924.566 kb on + strand at 924.616 kb on - strand at 924.616 kb on - strand at 924.644 kb on + strand at 924.644 kb on + strand at 924.734 kb on + strand at 924.734 kb on + strand at 924.765 kb on - strand at 924.780 kb on + strand at 924.954 kb on + strand at 925.246 kb on - strand at 925.246 kb on - strand at 925.319 kb on + strand at 925.319 kb on + strand at 925.319 kb on + strand at 925.327 kb on - strand at 925.686 kb on - strand at 925.687 kb on - strand at 925.687 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 3 remove 921,790 + cspD b0880 0.89 +0.7 921,790 + cspD b0880 0.89 -0.5 921,956 - +0.2 921,956 - +0.7 922,042 + -0.7 922,042 + -0.2 922,055 + -0.6 922,069 + -0.8 922,091 - -1.6 922,103 + -1.0 922,124 + -0.7 922,246 - clpS b0881 0.34 +0.5 922,417 + clpS b0881 0.88 +0.6 922,435 - +0.1 922,562 + +1.5 922,686 - +1.4 922,695 - +0.9 922,703 + +1.5 922,703 + +1.0 922,745 + clpA b0882 0.11 +1.6 922,745 + clpA b0882 0.11 +0.3 922,767 + clpA b0882 0.12 +1.3 922,767 + clpA b0882 0.12 +1.7 922,850 + clpA b0882 0.16 +1.4 922,877 - clpA b0882 0.17 +1.8 922,904 - clpA b0882 0.18 +1.7 923,228 + clpA b0882 0.33 +1.4 923,236 - clpA b0882 0.33 +1.5 923,381 + clpA b0882 0.39 +0.8 923,462 - clpA b0882 0.43 +1.7 923,523 + clpA b0882 0.45 +1.5 923,523 + clpA b0882 0.45 +1.0 923,595 - clpA b0882 0.49 +1.4 923,595 - clpA b0882 0.49 +2.3 923,644 + clpA b0882 0.51 +1.1 923,666 - clpA b0882 0.52 +1.9 923,736 - clpA b0882 0.55 +1.4 923,793 + clpA b0882 0.57 +1.5 923,903 + clpA b0882 0.62 +2.5 923,903 + clpA b0882 0.62 +1.3 923,924 - clpA b0882 0.63 +1.5 923,982 - clpA b0882 0.66 +1.8 923,999 + clpA b0882 0.66 +1.4 924,056 - clpA b0882 0.69 +1.2 924,056 - clpA b0882 0.69 +2.0 924,082 + clpA b0882 0.70 +1.4 924,093 - clpA b0882 0.71 +0.6 924,151 - clpA b0882 0.73 +2.2 924,151 - clpA b0882 0.73 +2.0 924,151 - clpA b0882 0.73 +2.0 924,344 + clpA b0882 0.82 +1.7 924,344 + clpA b0882 0.82 +1.3 924,542 + +2.0 924,566 + +2.4 924,616 - +1.7 924,616 - +1.9 924,644 + +1.8 924,644 + +1.3 924,734 + +1.2 924,734 + +1.0 924,765 - +0.6 924,780 + +1.5 924,954 + -0.5 925,246 - -0.9 925,246 - -0.1 925,319 + +0.3 925,319 + -0.1 925,319 + +0.1 925,327 - +0.1 925,686 - +0.8 925,687 - -0.4 925,687 - +0.6
Or see this region's nucleotide sequence