Experiment: Ying_sugar7 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybcF and purK are separated by 194 nucleotides purK and purE overlap by 4 nucleotides purE and lpxH are separated by 117 nucleotides
b0521: ybcF - predicted carbamate kinase (NCBI), at 549,662 to 550,555
ybcF
b0522: purK - phosphoribosylaminoimidazole carboxylase (NCBI), at 550,750 to 551,817
purK
b0523: purE - phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI), at 551,814 to 552,323
purE
b0524: lpxH - UDP-2,3-diacylglucosamine hydrolase (NCBI), at 552,441 to 553,163
lpxH
Position (kb) 550
551
552 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 549.755 kb on + strand, within ybcF at 549.755 kb on + strand, within ybcF at 549.764 kb on + strand, within ybcF at 549.811 kb on - strand, within ybcF at 549.933 kb on + strand, within ybcF at 549.933 kb on + strand, within ybcF at 549.954 kb on - strand, within ybcF at 550.141 kb on - strand, within ybcF at 550.363 kb on + strand, within ybcF at 550.471 kb on - strand at 550.539 kb on + strand at 550.550 kb on - strand at 550.563 kb on + strand at 550.563 kb on - strand at 550.607 kb on + strand at 550.677 kb on + strand at 550.697 kb on - strand at 550.746 kb on + strand at 550.755 kb on - strand at 550.765 kb on - strand at 550.959 kb on + strand, within purK at 550.977 kb on - strand, within purK at 551.057 kb on + strand, within purK at 551.062 kb on + strand, within purK at 551.062 kb on + strand, within purK at 551.091 kb on - strand, within purK at 551.095 kb on + strand, within purK at 551.095 kb on + strand, within purK at 551.112 kb on + strand, within purK at 551.112 kb on + strand, within purK at 551.144 kb on + strand, within purK at 551.162 kb on - strand, within purK at 551.162 kb on - strand, within purK at 551.217 kb on + strand, within purK at 551.227 kb on - strand, within purK at 551.236 kb on + strand, within purK at 551.236 kb on + strand, within purK at 551.255 kb on + strand, within purK at 551.271 kb on + strand, within purK at 551.356 kb on + strand, within purK at 551.375 kb on + strand, within purK at 551.391 kb on - strand, within purK at 551.458 kb on - strand, within purK at 551.532 kb on + strand, within purK at 551.532 kb on + strand, within purK at 551.543 kb on + strand, within purK at 551.591 kb on - strand, within purK at 551.591 kb on - strand, within purK at 551.649 kb on - strand, within purK at 551.772 kb on + strand at 551.878 kb on + strand, within purE at 552.015 kb on - strand, within purE at 552.027 kb on + strand, within purE at 552.035 kb on - strand, within purE at 552.052 kb on - strand, within purE at 552.252 kb on + strand, within purE at 552.350 kb on + strand at 552.350 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 3 remove 549,755 + ybcF b0521 0.10 -0.9 549,755 + ybcF b0521 0.10 -0.5 549,764 + ybcF b0521 0.11 -0.4 549,811 - ybcF b0521 0.17 -0.1 549,933 + ybcF b0521 0.30 +0.7 549,933 + ybcF b0521 0.30 -0.6 549,954 - ybcF b0521 0.33 +0.5 550,141 - ybcF b0521 0.54 +0.7 550,363 + ybcF b0521 0.78 +0.9 550,471 - -0.0 550,539 + +0.0 550,550 - -1.5 550,563 + +0.1 550,563 - +0.2 550,607 + +0.9 550,677 + +0.2 550,697 - +0.1 550,746 + +0.1 550,755 - +0.4 550,765 - +0.4 550,959 + purK b0522 0.20 -2.5 550,977 - purK b0522 0.21 -5.6 551,057 + purK b0522 0.29 -4.2 551,062 + purK b0522 0.29 -4.3 551,062 + purK b0522 0.29 -4.0 551,091 - purK b0522 0.32 -4.7 551,095 + purK b0522 0.32 -0.8 551,095 + purK b0522 0.32 -2.9 551,112 + purK b0522 0.34 -4.1 551,112 + purK b0522 0.34 -3.3 551,144 + purK b0522 0.37 -3.4 551,162 - purK b0522 0.39 -3.3 551,162 - purK b0522 0.39 -3.9 551,217 + purK b0522 0.44 -3.4 551,227 - purK b0522 0.45 -2.3 551,236 + purK b0522 0.46 -5.1 551,236 + purK b0522 0.46 -4.3 551,255 + purK b0522 0.47 -2.9 551,271 + purK b0522 0.49 -3.3 551,356 + purK b0522 0.57 -5.3 551,375 + purK b0522 0.59 -5.1 551,391 - purK b0522 0.60 -3.6 551,458 - purK b0522 0.66 -5.8 551,532 + purK b0522 0.73 -4.1 551,532 + purK b0522 0.73 -5.2 551,543 + purK b0522 0.74 -3.9 551,591 - purK b0522 0.79 -3.7 551,591 - purK b0522 0.79 -3.9 551,649 - purK b0522 0.84 -3.9 551,772 + -1.9 551,878 + purE b0523 0.13 -3.4 552,015 - purE b0523 0.39 -5.1 552,027 + purE b0523 0.42 -2.9 552,035 - purE b0523 0.43 -4.8 552,052 - purE b0523 0.47 -4.9 552,252 + purE b0523 0.86 -4.1 552,350 + -4.0 552,350 + -3.2
Or see this region's nucleotide sequence