Experiment: Ying_sugar7 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dxs and ispA are separated by 24 nucleotides ispA and xseB overlap by 1 nucleotides xseB and thiI are separated by 205 nucleotides
b0420: dxs - 1-deoxy-D-xylulose-5-phosphate synthase (NCBI), at 437,539 to 439,401
dxs
b0421: ispA - geranyltranstransferase (NCBI), at 439,426 to 440,325
ispA
b0422: xseB - exodeoxyribonuclease VII small subunit (NCBI), at 440,325 to 440,567
xseB
b0423: thiI - thiamine biosynthesis protein ThiI (NCBI), at 440,773 to 442,221
thiI
Position (kb) 439
440
441 Strain fitness (log2 ratio) -1
0
1
2 at 439.386 kb on - strand at 439.407 kb on - strand at 440.335 kb on - strand at 440.546 kb on - strand at 440.581 kb on - strand at 440.581 kb on - strand at 440.609 kb on + strand at 440.650 kb on + strand at 440.650 kb on + strand at 440.650 kb on - strand at 440.716 kb on - strand at 440.750 kb on - strand at 440.882 kb on + strand at 440.882 kb on + strand at 440.882 kb on + strand at 440.890 kb on - strand at 440.890 kb on - strand at 440.968 kb on + strand, within thiI at 440.968 kb on + strand, within thiI at 440.976 kb on - strand, within thiI at 440.976 kb on - strand, within thiI at 440.976 kb on - strand, within thiI at 441.031 kb on - strand, within thiI at 441.031 kb on - strand, within thiI at 441.040 kb on - strand, within thiI at 441.066 kb on - strand, within thiI at 441.100 kb on + strand, within thiI
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 3 remove 439,386 - +0.8 439,407 - +0.5 440,335 - +0.7 440,546 - +0.7 440,581 - +0.0 440,581 - +0.3 440,609 + -1.1 440,650 + +0.6 440,650 + +0.5 440,650 - +0.5 440,716 - -1.1 440,750 - -0.6 440,882 + +0.3 440,882 + +0.8 440,882 + -0.7 440,890 - -0.8 440,890 - +0.7 440,968 + thiI b0423 0.13 +0.2 440,968 + thiI b0423 0.13 +1.8 440,976 - thiI b0423 0.14 +0.8 440,976 - thiI b0423 0.14 -0.4 440,976 - thiI b0423 0.14 -1.4 441,031 - thiI b0423 0.18 +0.9 441,031 - thiI b0423 0.18 +0.8 441,040 - thiI b0423 0.18 +0.2 441,066 - thiI b0423 0.20 +0.1 441,100 + thiI b0423 0.23 -0.2
Or see this region's nucleotide sequence