Strain Fitness in Escherichia coli BW25113 around b4425

Experiment: Ying_sugar7 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnarI and rttR are separated by 539 nucleotidesrttR and tpr overlap by 150 nucleotidestpr and tyrV are separated by 67 nucleotidestyrV and tyrT are separated by 209 nucleotidestyrT and purU are separated by 159 nucleotides b1227: narI - nitrate reductase 1, gamma (cytochrome b(NR)) subunit (NCBI), at 1,285,072 to 1,285,749 narI b4425: rttR - regulatory sRNA (NCBI), at 1,286,289 to 1,286,459 rttR b1229: tpr - predicted protamine-like protein (RefSeq), at 1,286,310 to 1,286,399 tpr b1230: tyrV - tRNA-Tyr (NCBI), at 1,286,467 to 1,286,551 tyrV b1231: tyrT - tRNA-Tyr (NCBI), at 1,286,761 to 1,286,845 tyrT b1232: purU - formyltetrahydrofolate deformylase (NCBI), at 1,287,005 to 1,287,847 purU Position (kb) 1286 1287Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 1285.352 kb on - strand, within narIat 1285.352 kb on - strand, within narIat 1285.476 kb on + strand, within narIat 1285.509 kb on - strand, within narIat 1285.518 kb on + strand, within narIat 1285.518 kb on + strand, within narIat 1285.526 kb on - strand, within narIat 1285.605 kb on - strand, within narIat 1285.670 kb on - strand, within narIat 1285.686 kb on + strandat 1285.694 kb on - strandat 1285.707 kb on + strandat 1286.125 kb on + strandat 1286.316 kb on - strandat 1286.316 kb on - strandat 1286.690 kb on - strandat 1286.690 kb on - strandat 1286.805 kb on + strand, within tyrTat 1286.916 kb on + strandat 1287.056 kb on + strandat 1287.064 kb on + strandat 1287.233 kb on + strand, within purUat 1287.255 kb on - strand, within purUat 1287.255 kb on - strand, within purUat 1287.342 kb on + strand, within purUat 1287.375 kb on + strand, within purUat 1287.423 kb on - strand, within purUat 1287.425 kb on + strand, within purUat 1287.439 kb on + strand, within purUat 1287.448 kb on + strand, within purU

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 3
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1,285,352 - narI b1227 0.41 +0.1
1,285,352 - narI b1227 0.41 -0.2
1,285,476 + narI b1227 0.60 +0.3
1,285,509 - narI b1227 0.64 -0.4
1,285,518 + narI b1227 0.66 -0.5
1,285,518 + narI b1227 0.66 -0.0
1,285,526 - narI b1227 0.67 -0.4
1,285,605 - narI b1227 0.79 -0.2
1,285,670 - narI b1227 0.88 +0.2
1,285,686 + +0.0
1,285,694 - +0.1
1,285,707 + -0.0
1,286,125 + +0.5
1,286,316 - -0.1
1,286,316 - +0.0
1,286,690 - +0.3
1,286,690 - +0.3
1,286,805 + tyrT b1231 0.52 -1.0
1,286,916 + -2.0
1,287,056 + -4.7
1,287,064 + -6.1
1,287,233 + purU b1232 0.27 -3.2
1,287,255 - purU b1232 0.30 -5.5
1,287,255 - purU b1232 0.30 -3.3
1,287,342 + purU b1232 0.40 -3.7
1,287,375 + purU b1232 0.44 -1.8
1,287,423 - purU b1232 0.50 -3.5
1,287,425 + purU b1232 0.50 -2.6
1,287,439 + purU b1232 0.51 -3.8
1,287,448 + purU b1232 0.53 -3.9

Or see this region's nucleotide sequence