Experiment: Ying_sugar7 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metQ and metI are separated by 39 nucleotides metI and metN overlap by 8 nucleotides metN and gmhB are separated by 187 nucleotides
b0197: metQ - DL-methionine transporter subunit (NCBI), at 220,113 to 220,928
metQ
b0198: metI - DL-methionine transporter subunit (NCBI), at 220,968 to 221,621
metI
b0199: metN - DL-methionine transporter subunit (NCBI), at 221,614 to 222,645
metN
b0200: gmhB - hypothetical protein (NCBI), at 222,833 to 223,408
gmhB
Position (kb) 221
222
223 Strain fitness (log2 ratio) -2
-1
0
1 at 220.661 kb on + strand, within metQ at 220.675 kb on + strand, within metQ at 220.694 kb on - strand, within metQ at 220.694 kb on - strand, within metQ at 220.750 kb on - strand, within metQ at 220.754 kb on + strand, within metQ at 220.764 kb on + strand, within metQ at 220.825 kb on + strand, within metQ at 220.865 kb on + strand at 221.043 kb on - strand, within metI at 221.047 kb on + strand, within metI at 221.052 kb on + strand, within metI at 221.060 kb on - strand, within metI at 221.076 kb on + strand, within metI at 221.076 kb on + strand, within metI at 221.080 kb on + strand, within metI at 221.080 kb on + strand, within metI at 221.096 kb on - strand, within metI at 221.105 kb on - strand, within metI at 221.106 kb on - strand, within metI at 221.128 kb on - strand, within metI at 221.148 kb on + strand, within metI at 221.148 kb on + strand, within metI at 221.181 kb on + strand, within metI at 221.214 kb on + strand, within metI at 221.238 kb on + strand, within metI at 221.238 kb on + strand, within metI at 221.250 kb on - strand, within metI at 221.269 kb on - strand, within metI at 221.280 kb on + strand, within metI at 221.300 kb on - strand, within metI at 221.347 kb on - strand, within metI at 221.383 kb on - strand, within metI at 221.383 kb on - strand, within metI at 221.466 kb on - strand, within metI at 221.469 kb on - strand, within metI at 221.511 kb on - strand, within metI at 221.511 kb on - strand, within metI at 221.595 kb on - strand at 221.595 kb on - strand at 221.619 kb on + strand at 221.623 kb on + strand at 221.655 kb on - strand at 221.680 kb on + strand at 221.685 kb on + strand at 221.688 kb on - strand at 221.802 kb on + strand, within metN at 221.830 kb on + strand, within metN at 221.830 kb on + strand, within metN at 221.846 kb on + strand, within metN at 221.847 kb on + strand, within metN at 221.848 kb on + strand, within metN at 221.856 kb on + strand, within metN at 221.911 kb on - strand, within metN at 221.967 kb on + strand, within metN at 221.967 kb on + strand, within metN at 221.975 kb on - strand, within metN at 221.999 kb on - strand, within metN at 222.001 kb on + strand, within metN at 222.009 kb on - strand, within metN at 222.027 kb on - strand, within metN at 222.061 kb on - strand, within metN at 222.102 kb on - strand, within metN at 222.102 kb on - strand, within metN at 222.159 kb on + strand, within metN at 222.203 kb on + strand, within metN at 222.239 kb on + strand, within metN at 222.239 kb on + strand, within metN at 222.247 kb on + strand, within metN at 222.269 kb on - strand, within metN at 222.279 kb on - strand, within metN at 222.364 kb on - strand, within metN at 222.425 kb on - strand, within metN at 222.495 kb on - strand, within metN at 222.501 kb on + strand, within metN at 222.501 kb on + strand, within metN at 222.507 kb on - strand, within metN at 222.511 kb on + strand, within metN at 222.520 kb on - strand, within metN at 222.578 kb on - strand at 222.588 kb on - strand at 222.588 kb on - strand at 222.594 kb on - strand at 222.594 kb on - strand at 222.620 kb on - strand at 222.731 kb on - strand at 222.732 kb on + strand at 222.732 kb on + strand at 222.813 kb on + strand at 222.907 kb on - strand, within gmhB at 222.907 kb on - strand, within gmhB at 222.941 kb on - strand, within gmhB at 223.224 kb on + strand, within gmhB at 223.232 kb on - strand, within gmhB at 223.232 kb on - strand, within gmhB at 223.477 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 3 remove 220,661 + metQ b0197 0.67 +0.7 220,675 + metQ b0197 0.69 +0.5 220,694 - metQ b0197 0.71 -0.8 220,694 - metQ b0197 0.71 -0.2 220,750 - metQ b0197 0.78 -0.1 220,754 + metQ b0197 0.79 +0.7 220,764 + metQ b0197 0.80 -0.5 220,825 + metQ b0197 0.87 +1.0 220,865 + +0.7 221,043 - metI b0198 0.11 +0.4 221,047 + metI b0198 0.12 +0.2 221,052 + metI b0198 0.13 +0.1 221,060 - metI b0198 0.14 -0.3 221,076 + metI b0198 0.17 +0.1 221,076 + metI b0198 0.17 +0.9 221,080 + metI b0198 0.17 +0.8 221,080 + metI b0198 0.17 -1.4 221,096 - metI b0198 0.20 -0.5 221,105 - metI b0198 0.21 +0.3 221,106 - metI b0198 0.21 +0.2 221,128 - metI b0198 0.24 -0.3 221,148 + metI b0198 0.28 +0.9 221,148 + metI b0198 0.28 +0.5 221,181 + metI b0198 0.33 -0.0 221,214 + metI b0198 0.38 +1.0 221,238 + metI b0198 0.41 -0.1 221,238 + metI b0198 0.41 +0.5 221,250 - metI b0198 0.43 +0.3 221,269 - metI b0198 0.46 +0.4 221,280 + metI b0198 0.48 -0.0 221,300 - metI b0198 0.51 +1.0 221,347 - metI b0198 0.58 -0.5 221,383 - metI b0198 0.63 +0.6 221,383 - metI b0198 0.63 +0.6 221,466 - metI b0198 0.76 +0.4 221,469 - metI b0198 0.77 +0.4 221,511 - metI b0198 0.83 +0.5 221,511 - metI b0198 0.83 +0.3 221,595 - -0.1 221,595 - +0.6 221,619 + +0.7 221,623 + +0.5 221,655 - +0.3 221,680 + +0.8 221,685 + +0.4 221,688 - +0.4 221,802 + metN b0199 0.18 +0.6 221,830 + metN b0199 0.21 +0.8 221,830 + metN b0199 0.21 +0.8 221,846 + metN b0199 0.22 -0.1 221,847 + metN b0199 0.23 +0.9 221,848 + metN b0199 0.23 +0.6 221,856 + metN b0199 0.23 +1.0 221,911 - metN b0199 0.29 +0.4 221,967 + metN b0199 0.34 +0.7 221,967 + metN b0199 0.34 +0.6 221,975 - metN b0199 0.35 +0.8 221,999 - metN b0199 0.37 +0.8 222,001 + metN b0199 0.38 +0.9 222,009 - metN b0199 0.38 -0.6 222,027 - metN b0199 0.40 +0.2 222,061 - metN b0199 0.43 +0.6 222,102 - metN b0199 0.47 +0.8 222,102 - metN b0199 0.47 +0.6 222,159 + metN b0199 0.53 -0.0 222,203 + metN b0199 0.57 +0.1 222,239 + metN b0199 0.61 +0.4 222,239 + metN b0199 0.61 +1.0 222,247 + metN b0199 0.61 +1.7 222,269 - metN b0199 0.63 +0.4 222,279 - metN b0199 0.64 -0.3 222,364 - metN b0199 0.73 +0.5 222,425 - metN b0199 0.79 +0.7 222,495 - metN b0199 0.85 +0.8 222,501 + metN b0199 0.86 +0.7 222,501 + metN b0199 0.86 +0.7 222,507 - metN b0199 0.87 +0.6 222,511 + metN b0199 0.87 +0.4 222,520 - metN b0199 0.88 -0.3 222,578 - +0.1 222,588 - +0.3 222,588 - +0.1 222,594 - +0.8 222,594 - -0.3 222,620 - +0.5 222,731 - -0.0 222,732 + +0.2 222,732 + +0.4 222,813 + +0.2 222,907 - gmhB b0200 0.13 -0.7 222,907 - gmhB b0200 0.13 -0.5 222,941 - gmhB b0200 0.19 -2.4 223,224 + gmhB b0200 0.68 -0.9 223,232 - gmhB b0200 0.69 -0.7 223,232 - gmhB b0200 0.69 -0.5 223,477 - +0.3
Or see this region's nucleotide sequence