Experiment: Ying_OrganicAcid7 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bcp and hyfA are separated by 252 nucleotides hyfA and hyfB overlap by 1 nucleotides hyfB and hyfC are separated by 10 nucleotides hyfC and hyfD are separated by 16 nucleotides
b2480: bcp - thioredoxin-dependent thiol peroxidase (NCBI), at 2,598,500 to 2,598,970
bcp
b2481: hyfA - hydrogenase 4 Fe-S subunit (VIMSS), at 2,599,223 to 2,599,840
hyfA
b2482: hyfB - NADH dehydrogenase subunit N (NCBI), at 2,599,840 to 2,601,858
hyfB
b2483: hyfC - hydrogenase 4 membrane subunit (VIMSS), at 2,601,869 to 2,602,816
hyfC
b2484: hyfD - hydrogenase 4 membrane subunit (NCBI), at 2,602,833 to 2,604,272
hyfD
Position (kb)
2599
2600
2601
2602 Strain fitness (log2 ratio)
-2
-1
0
1 at 2598.938 kb on + strand at 2598.967 kb on - strand at 2598.976 kb on - strand at 2599.005 kb on + strand at 2599.230 kb on + strand at 2599.238 kb on - strand at 2599.319 kb on - strand, within hyfA at 2599.319 kb on - strand, within hyfA at 2599.362 kb on + strand, within hyfA at 2599.370 kb on - strand, within hyfA at 2599.426 kb on + strand, within hyfA at 2599.488 kb on - strand, within hyfA at 2599.528 kb on - strand, within hyfA at 2599.913 kb on - strand at 2599.998 kb on + strand at 2600.132 kb on + strand, within hyfB at 2600.135 kb on - strand, within hyfB at 2600.340 kb on - strand, within hyfB at 2600.517 kb on - strand, within hyfB at 2600.527 kb on + strand, within hyfB at 2600.577 kb on - strand, within hyfB at 2600.621 kb on - strand, within hyfB at 2600.621 kb on - strand, within hyfB at 2600.759 kb on - strand, within hyfB at 2600.889 kb on + strand, within hyfB at 2601.194 kb on + strand, within hyfB at 2601.202 kb on - strand, within hyfB at 2601.245 kb on - strand, within hyfB at 2601.245 kb on - strand, within hyfB at 2601.404 kb on - strand, within hyfB at 2601.421 kb on + strand, within hyfB at 2601.434 kb on + strand, within hyfB at 2601.588 kb on + strand, within hyfB at 2601.644 kb on + strand, within hyfB at 2601.644 kb on + strand, within hyfB at 2601.652 kb on - strand, within hyfB at 2601.652 kb on - strand, within hyfB at 2601.701 kb on - strand at 2601.701 kb on - strand at 2601.810 kb on + strand at 2601.925 kb on + strand at 2601.925 kb on + strand at 2601.988 kb on - strand, within hyfC at 2602.127 kb on + strand, within hyfC at 2602.127 kb on + strand, within hyfC at 2602.150 kb on - strand, within hyfC at 2602.199 kb on - strand, within hyfC at 2602.199 kb on - strand, within hyfC at 2602.325 kb on + strand, within hyfC at 2602.350 kb on + strand, within hyfC at 2602.379 kb on + strand, within hyfC at 2602.382 kb on - strand, within hyfC at 2602.432 kb on - strand, within hyfC at 2602.620 kb on - strand, within hyfC at 2602.620 kb on - strand, within hyfC at 2602.813 kb on + strand at 2602.813 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 3 remove 2,598,938 + -0.3 2,598,967 - +0.3 2,598,976 - +0.3 2,599,005 + +0.1 2,599,230 + +0.4 2,599,238 - -0.6 2,599,319 - hyfA b2481 0.16 +0.2 2,599,319 - hyfA b2481 0.16 +0.6 2,599,362 + hyfA b2481 0.22 +0.2 2,599,370 - hyfA b2481 0.24 -0.2 2,599,426 + hyfA b2481 0.33 +0.4 2,599,488 - hyfA b2481 0.43 -0.0 2,599,528 - hyfA b2481 0.49 -0.8 2,599,913 - +0.4 2,599,998 + +0.2 2,600,132 + hyfB b2482 0.14 +0.2 2,600,135 - hyfB b2482 0.15 -0.8 2,600,340 - hyfB b2482 0.25 -0.2 2,600,517 - hyfB b2482 0.34 +0.2 2,600,527 + hyfB b2482 0.34 -0.4 2,600,577 - hyfB b2482 0.37 +0.3 2,600,621 - hyfB b2482 0.39 -0.2 2,600,621 - hyfB b2482 0.39 -0.0 2,600,759 - hyfB b2482 0.46 +0.5 2,600,889 + hyfB b2482 0.52 +0.6 2,601,194 + hyfB b2482 0.67 +0.2 2,601,202 - hyfB b2482 0.67 -0.1 2,601,245 - hyfB b2482 0.70 +0.1 2,601,245 - hyfB b2482 0.70 -0.2 2,601,404 - hyfB b2482 0.77 +1.4 2,601,421 + hyfB b2482 0.78 -1.1 2,601,434 + hyfB b2482 0.79 +0.3 2,601,588 + hyfB b2482 0.87 -0.3 2,601,644 + hyfB b2482 0.89 +1.2 2,601,644 + hyfB b2482 0.89 +0.2 2,601,652 - hyfB b2482 0.90 +0.3 2,601,652 - hyfB b2482 0.90 +0.0 2,601,701 - -2.6 2,601,701 - +0.4 2,601,810 + +0.3 2,601,925 + +1.2 2,601,925 + +0.4 2,601,988 - hyfC b2483 0.13 +0.0 2,602,127 + hyfC b2483 0.27 +0.0 2,602,127 + hyfC b2483 0.27 +0.9 2,602,150 - hyfC b2483 0.30 -0.0 2,602,199 - hyfC b2483 0.35 +0.3 2,602,199 - hyfC b2483 0.35 -0.6 2,602,325 + hyfC b2483 0.48 +0.6 2,602,350 + hyfC b2483 0.51 -0.2 2,602,379 + hyfC b2483 0.54 +0.7 2,602,382 - hyfC b2483 0.54 -0.5 2,602,432 - hyfC b2483 0.59 +0.8 2,602,620 - hyfC b2483 0.79 -0.9 2,602,620 - hyfC b2483 0.79 -0.2 2,602,813 + +0.4 2,602,813 + +0.6
Or see this region's nucleotide sequence