Strain Fitness in Escherichia coli BW25113 around b2262

Experiment: Ying_OrganicAcid7 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmenE and menC overlap by 4 nucleotidesmenC and menB overlap by 1 nucleotidesmenB and yfbB are separated by 14 nucleotidesyfbB and menD overlap by 4 nucleotides b2260: menE - O-succinylbenzoic acid--CoA ligase (NCBI), at 2,371,670 to 2,373,025 menE b2261: menC - O-succinylbenzoate synthase (NCBI), at 2,373,022 to 2,373,984 menC b2262: menB - naphthoate synthase (NCBI), at 2,373,984 to 2,374,841 menB b2263: yfbB - predicted peptidase (NCBI), at 2,374,856 to 2,375,614 yfbB b2264: menD - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI), at 2,375,611 to 2,377,281 menD Position (kb) 2373 2374 2375Strain fitness (log2 ratio) -1 0 1 2at 2372.986 kb on + strandat 2373.079 kb on + strandat 2373.079 kb on + strandat 2373.115 kb on + strandat 2373.146 kb on - strand, within menCat 2373.146 kb on - strand, within menCat 2373.146 kb on - strand, within menCat 2373.241 kb on - strand, within menCat 2373.255 kb on + strand, within menCat 2373.493 kb on - strand, within menCat 2373.493 kb on - strand, within menCat 2373.550 kb on - strand, within menCat 2373.559 kb on + strand, within menCat 2373.559 kb on + strand, within menCat 2373.733 kb on + strand, within menCat 2373.733 kb on + strand, within menCat 2373.743 kb on - strand, within menCat 2373.743 kb on - strand, within menCat 2373.925 kb on + strandat 2373.930 kb on + strandat 2373.930 kb on + strandat 2374.136 kb on + strand, within menBat 2374.254 kb on - strand, within menBat 2374.373 kb on + strand, within menBat 2374.491 kb on + strand, within menBat 2374.673 kb on - strand, within menBat 2374.831 kb on + strandat 2374.831 kb on + strandat 2374.885 kb on + strandat 2374.893 kb on - strandat 2375.223 kb on - strand, within yfbBat 2375.307 kb on - strand, within yfbBat 2375.363 kb on - strand, within yfbBat 2375.570 kb on + strandat 2375.570 kb on + strandat 2375.616 kb on - strandat 2375.616 kb on - strandat 2375.795 kb on - strand, within menD

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 3
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2,372,986 + -0.1
2,373,079 + -1.7
2,373,079 + +0.9
2,373,115 + +0.6
2,373,146 - menC b2261 0.13 +0.2
2,373,146 - menC b2261 0.13 +0.3
2,373,146 - menC b2261 0.13 -1.1
2,373,241 - menC b2261 0.23 -0.1
2,373,255 + menC b2261 0.24 -0.8
2,373,493 - menC b2261 0.49 +0.3
2,373,493 - menC b2261 0.49 +0.3
2,373,550 - menC b2261 0.55 -0.5
2,373,559 + menC b2261 0.56 +0.5
2,373,559 + menC b2261 0.56 +0.5
2,373,733 + menC b2261 0.74 -0.2
2,373,733 + menC b2261 0.74 +0.4
2,373,743 - menC b2261 0.75 -0.3
2,373,743 - menC b2261 0.75 +0.4
2,373,925 + +0.0
2,373,930 + +0.6
2,373,930 + -0.5
2,374,136 + menB b2262 0.18 -0.3
2,374,254 - menB b2262 0.31 +0.8
2,374,373 + menB b2262 0.45 -0.2
2,374,491 + menB b2262 0.59 +0.3
2,374,673 - menB b2262 0.80 +2.0
2,374,831 + +0.0
2,374,831 + -1.0
2,374,885 + +0.2
2,374,893 - -0.5
2,375,223 - yfbB b2263 0.48 -0.0
2,375,307 - yfbB b2263 0.59 +0.3
2,375,363 - yfbB b2263 0.67 -0.5
2,375,570 + -0.6
2,375,570 + +0.1
2,375,616 - +0.3
2,375,616 - +0.1
2,375,795 - menD b2264 0.11 +1.1

Or see this region's nucleotide sequence