Strain Fitness in Escherichia coli BW25113 around b1395

Experiment: Ying_OrganicAcid7 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpaaF and paaG overlap by 1 nucleotidespaaG and paaH are separated by 1 nucleotidespaaH and paaI overlap by 11 nucleotidespaaI and paaJ overlap by 1 nucleotides b1393: paaF - enoyl-CoA hydratase-isomerase (NCBI), at 1,455,521 to 1,456,288 paaF b1394: paaG - enoyl-CoA hydratase (NCBI), at 1,456,288 to 1,457,076 paaG b1395: paaH - 3-hydroxybutyryl-CoA dehydrogenase (NCBI), at 1,457,078 to 1,458,505 paaH b1396: paaI - predicted thioesterase (NCBI), at 1,458,495 to 1,458,917 paaI b1397: paaJ - acetyl-CoA acetyltransferase (NCBI), at 1,458,917 to 1,460,122 paaJ Position (kb) 1457 1458 1459Strain fitness (log2 ratio) -1 0 1at 1456.216 kb on - strandat 1456.216 kb on - strandat 1456.490 kb on + strand, within paaGat 1456.637 kb on + strand, within paaGat 1456.780 kb on - strand, within paaGat 1456.910 kb on + strand, within paaGat 1457.029 kb on + strandat 1457.029 kb on + strandat 1457.192 kb on + strandat 1457.264 kb on + strand, within paaHat 1457.625 kb on + strand, within paaHat 1457.625 kb on + strand, within paaHat 1457.668 kb on + strand, within paaHat 1457.750 kb on + strand, within paaHat 1457.822 kb on + strand, within paaHat 1457.921 kb on - strand, within paaHat 1457.921 kb on - strand, within paaHat 1457.941 kb on + strand, within paaHat 1457.979 kb on + strand, within paaHat 1458.061 kb on - strand, within paaHat 1458.061 kb on - strand, within paaHat 1458.104 kb on - strand, within paaHat 1458.104 kb on - strand, within paaHat 1458.138 kb on + strand, within paaHat 1458.207 kb on + strand, within paaHat 1458.487 kb on - strandat 1458.499 kb on + strandat 1458.558 kb on - strand, within paaIat 1458.590 kb on - strand, within paaIat 1458.590 kb on - strand, within paaIat 1458.602 kb on + strand, within paaIat 1458.602 kb on + strand, within paaIat 1458.679 kb on - strand, within paaIat 1458.689 kb on - strand, within paaIat 1458.786 kb on + strand, within paaIat 1458.909 kb on - strandat 1458.909 kb on - strandat 1458.914 kb on - strandat 1458.914 kb on - strandat 1459.088 kb on + strand, within paaJat 1459.188 kb on + strand, within paaJat 1459.188 kb on + strand, within paaJat 1459.425 kb on + strand, within paaJat 1459.425 kb on + strand, within paaJ

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 3
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1,456,216 - +0.3
1,456,216 - -0.3
1,456,490 + paaG b1394 0.26 +0.2
1,456,637 + paaG b1394 0.44 +0.8
1,456,780 - paaG b1394 0.62 +0.3
1,456,910 + paaG b1394 0.79 +0.8
1,457,029 + +0.8
1,457,029 + +0.7
1,457,192 + +0.1
1,457,264 + paaH b1395 0.13 +0.7
1,457,625 + paaH b1395 0.38 -0.0
1,457,625 + paaH b1395 0.38 +0.3
1,457,668 + paaH b1395 0.41 +0.3
1,457,750 + paaH b1395 0.47 +0.7
1,457,822 + paaH b1395 0.52 +0.6
1,457,921 - paaH b1395 0.59 +0.2
1,457,921 - paaH b1395 0.59 +0.8
1,457,941 + paaH b1395 0.60 +0.4
1,457,979 + paaH b1395 0.63 +0.4
1,458,061 - paaH b1395 0.69 +1.0
1,458,061 - paaH b1395 0.69 +0.8
1,458,104 - paaH b1395 0.72 -0.0
1,458,104 - paaH b1395 0.72 +0.9
1,458,138 + paaH b1395 0.74 +0.8
1,458,207 + paaH b1395 0.79 -0.4
1,458,487 - +0.4
1,458,499 + +0.3
1,458,558 - paaI b1396 0.15 -0.0
1,458,590 - paaI b1396 0.22 +0.2
1,458,590 - paaI b1396 0.22 -0.3
1,458,602 + paaI b1396 0.25 +0.5
1,458,602 + paaI b1396 0.25 +0.4
1,458,679 - paaI b1396 0.43 +0.2
1,458,689 - paaI b1396 0.46 -0.0
1,458,786 + paaI b1396 0.69 +0.8
1,458,909 - +0.0
1,458,909 - +0.8
1,458,914 - +0.5
1,458,914 - -0.6
1,459,088 + paaJ b1397 0.14 -0.8
1,459,188 + paaJ b1397 0.22 -0.5
1,459,188 + paaJ b1397 0.22 +0.2
1,459,425 + paaJ b1397 0.42 -0.6
1,459,425 + paaJ b1397 0.42 -0.2

Or see this region's nucleotide sequence