Strain Fitness in Escherichia coli BW25113 around b1132

Experiment: Ying_OrganicAcid7 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpurB and hflD are separated by 3 nucleotideshflD and ycfB are separated by 35 nucleotides b1131: purB - adenylosuccinate lyase (NCBI), at 1,189,839 to 1,191,209 purB b1132: hflD - hypothetical protein (NCBI), at 1,191,213 to 1,191,854 hflD b1133: ycfB - orf, hypothetical protein (VIMSS), at 1,191,890 to 1,192,996 ycfB Position (kb) 1191 1192Strain fitness (log2 ratio) -1 0 1at 1191.335 kb on - strand, within hflDat 1191.604 kb on - strand, within hflDat 1191.744 kb on - strand, within hflDat 1191.776 kb on - strand, within hflDat 1191.776 kb on - strand, within hflDat 1191.780 kb on - strand, within hflD

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep C; time point 3
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1,191,335 - hflD b1132 0.19 +0.6
1,191,604 - hflD b1132 0.61 +0.1
1,191,744 - hflD b1132 0.83 +0.2
1,191,776 - hflD b1132 0.88 +0.0
1,191,776 - hflD b1132 0.88 +1.2
1,191,780 - hflD b1132 0.88 +0.9

Or see this region's nucleotide sequence