Strain Fitness in Escherichia coli BW25113 around b4441

Experiment: Ying_OrganicAcid7 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfhK and glmY are separated by 58 nucleotidesglmY and purL are separated by 315 nucleotides b2556: yfhK - putative 2-component sensor protein (VIMSS), at 2,687,693 to 2,689,120 yfhK b4441: glmY - sRNA activator of glmS mRNA (RefSeq), at 2,689,179 to 2,689,362 glmY b2557: purL - phosphoribosylformylglycinamidine synthase (NCBI), at 2,689,678 to 2,693,565 purL Position (kb) 2689 2690Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 2688.321 kb on - strand, within yfhKat 2688.421 kb on + strand, within yfhKat 2688.467 kb on + strand, within yfhKat 2688.526 kb on + strand, within yfhKat 2688.526 kb on + strand, within yfhKat 2688.566 kb on + strand, within yfhKat 2688.652 kb on + strand, within yfhKat 2688.674 kb on - strand, within yfhKat 2688.707 kb on - strand, within yfhKat 2688.732 kb on - strand, within yfhKat 2688.874 kb on + strand, within yfhKat 2688.874 kb on + strand, within yfhKat 2688.908 kb on + strand, within yfhKat 2688.912 kb on + strand, within yfhKat 2688.914 kb on - strand, within yfhKat 2688.914 kb on - strand, within yfhKat 2688.969 kb on + strand, within yfhKat 2689.027 kb on + strandat 2689.030 kb on - strandat 2689.155 kb on - strandat 2689.169 kb on + strandat 2689.250 kb on + strand, within glmYat 2689.677 kb on - strandat 2689.716 kb on + strandat 2689.716 kb on + strandat 2689.716 kb on + strandat 2689.724 kb on - strandat 2689.838 kb on - strandat 2689.838 kb on - strandat 2689.891 kb on + strandat 2689.959 kb on + strandat 2690.045 kb on + strandat 2690.136 kb on + strand, within purLat 2690.136 kb on + strand, within purLat 2690.199 kb on - strand, within purLat 2690.199 kb on - strand, within purLat 2690.209 kb on + strand, within purLat 2690.294 kb on + strand, within purLat 2690.299 kb on + strand, within purLat 2690.308 kb on - strand, within purLat 2690.356 kb on - strand, within purL

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 3
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2,688,321 - yfhK b2556 0.44 +0.2
2,688,421 + yfhK b2556 0.51 +0.0
2,688,467 + yfhK b2556 0.54 +0.4
2,688,526 + yfhK b2556 0.58 -0.1
2,688,526 + yfhK b2556 0.58 +0.3
2,688,566 + yfhK b2556 0.61 -0.1
2,688,652 + yfhK b2556 0.67 -0.0
2,688,674 - yfhK b2556 0.69 +0.4
2,688,707 - yfhK b2556 0.71 +0.2
2,688,732 - yfhK b2556 0.73 +0.3
2,688,874 + yfhK b2556 0.83 -2.6
2,688,874 + yfhK b2556 0.83 +0.3
2,688,908 + yfhK b2556 0.85 +0.2
2,688,912 + yfhK b2556 0.85 -0.5
2,688,914 - yfhK b2556 0.86 -1.0
2,688,914 - yfhK b2556 0.86 +0.1
2,688,969 + yfhK b2556 0.89 +0.7
2,689,027 + -0.1
2,689,030 - -0.1
2,689,155 - -0.1
2,689,169 + +0.2
2,689,250 + glmY b4441 0.39 -0.7
2,689,677 - +0.2
2,689,716 + -4.3
2,689,716 + -3.1
2,689,716 + -5.2
2,689,724 - -4.9
2,689,838 - -5.2
2,689,838 - -6.4
2,689,891 + -3.7
2,689,959 + -6.2
2,690,045 + -6.6
2,690,136 + purL b2557 0.12 -3.0
2,690,136 + purL b2557 0.12 -3.9
2,690,199 - purL b2557 0.13 -3.0
2,690,199 - purL b2557 0.13 -3.2
2,690,209 + purL b2557 0.14 -6.1
2,690,294 + purL b2557 0.16 -4.5
2,690,299 + purL b2557 0.16 -3.2
2,690,308 - purL b2557 0.16 -4.1
2,690,356 - purL b2557 0.17 -3.0

Or see this region's nucleotide sequence