Experiment: Ying_OrganicAcid7 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sapC and sapB overlap by 14 nucleotides sapB and sapA overlap by 4 nucleotides sapA and ymjA are separated by 312 nucleotides ymjA and ycjJ are separated by 133 nucleotides
b1292: sapC - predicted antimicrobial peptide transporter subunit (NCBI), at 1,351,652 to 1,352,542
sapC
b1293: sapB - predicted antimicrobial peptide transporter subunit (NCBI), at 1,352,529 to 1,353,494
sapB
b1294: sapA - predicted antimicrobial peptide transporter subunit (NCBI), at 1,353,491 to 1,355,134
sapA
b1295: ymjA - hypothetical protein (NCBI), at 1,355,447 to 1,355,692
ymjA
b1296: ycjJ - putative amino acid/amine transport protein (VIMSS), at 1,355,826 to 1,357,211
ycjJ
Position (kb)
1353
1354
1355
1356 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1352.770 kb on - strand, within sapB at 1352.770 kb on - strand, within sapB at 1352.790 kb on + strand, within sapB at 1353.131 kb on + strand, within sapB at 1353.522 kb on + strand at 1353.566 kb on - strand at 1353.747 kb on + strand, within sapA at 1353.747 kb on + strand, within sapA at 1353.825 kb on + strand, within sapA at 1353.825 kb on + strand, within sapA at 1353.841 kb on + strand, within sapA at 1353.841 kb on + strand, within sapA at 1353.844 kb on - strand, within sapA at 1353.890 kb on - strand, within sapA at 1353.890 kb on - strand, within sapA at 1353.912 kb on - strand, within sapA at 1353.987 kb on + strand, within sapA at 1354.118 kb on - strand, within sapA at 1354.136 kb on + strand, within sapA at 1354.136 kb on + strand, within sapA at 1354.136 kb on + strand, within sapA at 1354.136 kb on + strand, within sapA at 1354.320 kb on - strand, within sapA at 1354.330 kb on - strand, within sapA at 1354.427 kb on - strand, within sapA at 1354.486 kb on - strand, within sapA at 1354.486 kb on - strand, within sapA at 1354.640 kb on + strand, within sapA at 1354.789 kb on - strand, within sapA at 1354.915 kb on - strand, within sapA at 1354.915 kb on - strand, within sapA at 1354.998 kb on - strand at 1355.215 kb on + strand at 1355.225 kb on - strand at 1355.225 kb on - strand at 1355.370 kb on - strand at 1355.370 kb on - strand at 1355.445 kb on - strand at 1355.485 kb on + strand, within ymjA at 1355.488 kb on - strand, within ymjA at 1355.488 kb on - strand, within ymjA at 1355.507 kb on - strand, within ymjA at 1355.713 kb on - strand at 1355.723 kb on - strand at 1355.726 kb on - strand at 1355.728 kb on - strand at 1355.853 kb on - strand at 1355.862 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 3 remove 1,352,770 - sapB b1293 0.25 -0.4 1,352,770 - sapB b1293 0.25 -1.4 1,352,790 + sapB b1293 0.27 -2.6 1,353,131 + sapB b1293 0.62 -0.4 1,353,522 + -0.7 1,353,566 - +2.1 1,353,747 + sapA b1294 0.16 +1.3 1,353,747 + sapA b1294 0.16 +0.9 1,353,825 + sapA b1294 0.20 +0.8 1,353,825 + sapA b1294 0.20 +0.3 1,353,841 + sapA b1294 0.21 +0.1 1,353,841 + sapA b1294 0.21 -0.6 1,353,844 - sapA b1294 0.21 +2.6 1,353,890 - sapA b1294 0.24 +2.2 1,353,890 - sapA b1294 0.24 +2.0 1,353,912 - sapA b1294 0.26 +2.4 1,353,987 + sapA b1294 0.30 +1.2 1,354,118 - sapA b1294 0.38 +2.1 1,354,136 + sapA b1294 0.39 +0.7 1,354,136 + sapA b1294 0.39 +1.2 1,354,136 + sapA b1294 0.39 +1.2 1,354,136 + sapA b1294 0.39 +1.4 1,354,320 - sapA b1294 0.50 +1.0 1,354,330 - sapA b1294 0.51 +1.6 1,354,427 - sapA b1294 0.57 +2.1 1,354,486 - sapA b1294 0.61 +2.1 1,354,486 - sapA b1294 0.61 +1.9 1,354,640 + sapA b1294 0.70 -0.1 1,354,789 - sapA b1294 0.79 +2.2 1,354,915 - sapA b1294 0.87 +1.8 1,354,915 - sapA b1294 0.87 +2.0 1,354,998 - -0.2 1,355,215 + -0.9 1,355,225 - +0.1 1,355,225 - +0.4 1,355,370 - -0.2 1,355,370 - -0.1 1,355,445 - +0.1 1,355,485 + ymjA b1295 0.15 +0.1 1,355,488 - ymjA b1295 0.17 -0.1 1,355,488 - ymjA b1295 0.17 +0.1 1,355,507 - ymjA b1295 0.24 +0.6 1,355,713 - +0.2 1,355,723 - -0.0 1,355,726 - +1.2 1,355,728 - -0.2 1,355,853 - -0.6 1,355,862 + +0.9
Or see this region's nucleotide sequence