Strain Fitness in Escherichia coli BW25113 around b0678

Experiment: Ying_OrganicAcid7 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnagA and nagB are separated by 59 nucleotidesnagB and nagE are separated by 332 nucleotides b0677: nagA - N-acetylglucosamine-6-phosphate deacetylase (NCBI), at 700,826 to 701,974 nagA b0678: nagB - glucosamine-6-phosphate deaminase (NCBI), at 702,034 to 702,834 nagB b0679: nagE - fused N-acetyl glucosamine specific PTS enzyme: IIC, IIB , and IIA components (NCBI), at 703,167 to 705,113 nagE Position (kb) 702 703Strain fitness (log2 ratio) -2 -1 0 1at 701.074 kb on - strand, within nagAat 701.191 kb on + strand, within nagAat 701.244 kb on - strand, within nagAat 701.309 kb on - strand, within nagAat 701.309 kb on - strand, within nagAat 701.321 kb on + strand, within nagAat 701.327 kb on - strand, within nagAat 701.347 kb on - strand, within nagAat 701.382 kb on + strand, within nagAat 701.417 kb on + strand, within nagAat 701.498 kb on - strand, within nagAat 701.531 kb on - strand, within nagAat 701.565 kb on + strand, within nagAat 701.565 kb on + strand, within nagAat 701.648 kb on + strand, within nagAat 701.692 kb on + strand, within nagAat 701.694 kb on - strand, within nagAat 701.755 kb on + strand, within nagAat 701.766 kb on + strand, within nagAat 701.805 kb on - strand, within nagAat 701.829 kb on + strand, within nagAat 701.904 kb on - strandat 701.904 kb on - strandat 701.959 kb on + strandat 701.959 kb on + strandat 701.959 kb on + strandat 701.968 kb on + strandat 701.980 kb on - strandat 702.001 kb on + strandat 702.001 kb on + strandat 702.011 kb on - strandat 702.020 kb on - strandat 702.104 kb on + strandat 702.160 kb on - strand, within nagBat 702.160 kb on - strand, within nagBat 702.166 kb on + strand, within nagBat 702.180 kb on - strand, within nagBat 702.217 kb on - strand, within nagBat 702.250 kb on + strand, within nagBat 702.346 kb on + strand, within nagBat 702.619 kb on - strand, within nagBat 702.624 kb on + strand, within nagBat 702.624 kb on + strand, within nagBat 702.769 kb on + strandat 702.794 kb on + strandat 702.804 kb on + strandat 702.823 kb on + strandat 702.823 kb on + strandat 702.881 kb on + strandat 702.966 kb on + strandat 703.054 kb on + strandat 703.054 kb on + strandat 703.064 kb on + strandat 703.087 kb on + strandat 703.193 kb on + strandat 703.193 kb on + strandat 703.211 kb on + strandat 703.420 kb on + strand, within nagEat 703.464 kb on - strand, within nagEat 703.634 kb on + strand, within nagEat 703.639 kb on - strand, within nagEat 703.663 kb on + strand, within nagEat 703.692 kb on - strand, within nagEat 703.711 kb on - strand, within nagEat 703.711 kb on - strand, within nagEat 703.742 kb on + strand, within nagEat 703.786 kb on - strand, within nagEat 703.786 kb on - strand, within nagEat 703.797 kb on + strand, within nagEat 703.815 kb on + strand, within nagEat 703.817 kb on - strand, within nagE

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 3
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701,074 - nagA b0677 0.22 -0.9
701,191 + nagA b0677 0.32 +0.7
701,244 - nagA b0677 0.36 -0.5
701,309 - nagA b0677 0.42 -0.8
701,309 - nagA b0677 0.42 -0.3
701,321 + nagA b0677 0.43 +1.0
701,327 - nagA b0677 0.44 -1.0
701,347 - nagA b0677 0.45 -1.2
701,382 + nagA b0677 0.48 -0.8
701,417 + nagA b0677 0.51 -0.3
701,498 - nagA b0677 0.58 +0.0
701,531 - nagA b0677 0.61 -0.5
701,565 + nagA b0677 0.64 -0.1
701,565 + nagA b0677 0.64 -0.9
701,648 + nagA b0677 0.72 -0.1
701,692 + nagA b0677 0.75 +0.7
701,694 - nagA b0677 0.76 +0.2
701,755 + nagA b0677 0.81 -1.2
701,766 + nagA b0677 0.82 +0.3
701,805 - nagA b0677 0.85 -1.3
701,829 + nagA b0677 0.87 -0.4
701,904 - +0.6
701,904 - -0.3
701,959 + +0.1
701,959 + -1.8
701,959 + +0.0
701,968 + -1.1
701,980 - +0.1
702,001 + +0.2
702,001 + +0.0
702,011 - +0.2
702,020 - -0.1
702,104 + +0.2
702,160 - nagB b0678 0.16 -0.1
702,160 - nagB b0678 0.16 +0.8
702,166 + nagB b0678 0.16 +0.3
702,180 - nagB b0678 0.18 -0.4
702,217 - nagB b0678 0.23 -0.4
702,250 + nagB b0678 0.27 +0.4
702,346 + nagB b0678 0.39 -0.2
702,619 - nagB b0678 0.73 -0.1
702,624 + nagB b0678 0.74 -0.5
702,624 + nagB b0678 0.74 -0.4
702,769 + -0.2
702,794 + -0.2
702,804 + -0.3
702,823 + +0.2
702,823 + -1.0
702,881 + -0.1
702,966 + -0.3
703,054 + -0.6
703,054 + -1.6
703,064 + +0.3
703,087 + -0.6
703,193 + +0.6
703,193 + -0.6
703,211 + -0.0
703,420 + nagE b0679 0.13 +0.4
703,464 - nagE b0679 0.15 -0.1
703,634 + nagE b0679 0.24 +0.0
703,639 - nagE b0679 0.24 +0.0
703,663 + nagE b0679 0.25 +1.0
703,692 - nagE b0679 0.27 -0.6
703,711 - nagE b0679 0.28 -0.1
703,711 - nagE b0679 0.28 +0.6
703,742 + nagE b0679 0.30 +0.4
703,786 - nagE b0679 0.32 -0.1
703,786 - nagE b0679 0.32 +0.2
703,797 + nagE b0679 0.32 +0.1
703,815 + nagE b0679 0.33 +0.3
703,817 - nagE b0679 0.33 +0.4

Or see this region's nucleotide sequence