Strain Fitness in Escherichia coli BW25113 around b2422

Experiment: Ying_OrganicAcid7 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcysM and cysA are separated by 133 nucleotidescysA and cysW overlap by 11 nucleotidescysW and cysU overlap by 1 nucleotides b2421: cysM - cysteine synthase B (O-acetylserine sulfhydrolase B) (NCBI), at 2,536,694 to 2,537,605 cysM b2422: cysA - sulfate/thiosulfate transporter subunit (NCBI), at 2,537,739 to 2,538,836 cysA b2423: cysW - sulfate/thiosulfate transporter subunit (NCBI), at 2,538,826 to 2,539,701 cysW b2424: cysU - sulfate/thiosulfate transporter subunit (NCBI), at 2,539,701 to 2,540,534 cysU Position (kb) 2537 2538 2539Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 2536.906 kb on + strand, within cysMat 2537.014 kb on - strand, within cysMat 2537.014 kb on - strand, within cysMat 2537.041 kb on + strand, within cysMat 2537.041 kb on + strand, within cysMat 2537.148 kb on + strand, within cysMat 2537.148 kb on + strand, within cysMat 2537.578 kb on + strandat 2537.578 kb on + strandat 2537.849 kb on + strand, within cysAat 2537.862 kb on - strand, within cysAat 2537.903 kb on - strand, within cysAat 2537.963 kb on - strand, within cysAat 2538.033 kb on + strand, within cysAat 2538.037 kb on - strand, within cysAat 2538.054 kb on - strand, within cysAat 2538.054 kb on - strand, within cysAat 2538.084 kb on - strand, within cysAat 2538.108 kb on - strand, within cysAat 2538.114 kb on + strand, within cysAat 2538.193 kb on + strand, within cysAat 2538.193 kb on + strand, within cysAat 2538.224 kb on + strand, within cysAat 2538.224 kb on + strand, within cysAat 2538.334 kb on - strand, within cysAat 2538.334 kb on - strand, within cysAat 2538.336 kb on - strand, within cysAat 2538.699 kb on + strand, within cysAat 2538.824 kb on + strandat 2538.824 kb on + strandat 2538.846 kb on - strandat 2538.846 kb on - strandat 2538.952 kb on + strand, within cysWat 2538.952 kb on + strand, within cysWat 2538.959 kb on + strand, within cysWat 2538.961 kb on + strand, within cysWat 2538.986 kb on + strand, within cysWat 2539.072 kb on + strand, within cysWat 2539.173 kb on + strand, within cysWat 2539.173 kb on + strand, within cysWat 2539.240 kb on + strand, within cysWat 2539.249 kb on - strand, within cysWat 2539.249 kb on - strand, within cysWat 2539.456 kb on + strand, within cysWat 2539.468 kb on - strand, within cysWat 2539.468 kb on - strand, within cysWat 2539.468 kb on - strand, within cysWat 2539.694 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 3
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2,536,906 + cysM b2421 0.23 +0.8
2,537,014 - cysM b2421 0.35 +0.3
2,537,014 - cysM b2421 0.35 +1.0
2,537,041 + cysM b2421 0.38 +1.1
2,537,041 + cysM b2421 0.38 +0.9
2,537,148 + cysM b2421 0.50 +0.3
2,537,148 + cysM b2421 0.50 -0.0
2,537,578 + +0.7
2,537,578 + +1.0
2,537,849 + cysA b2422 0.10 -6.5
2,537,862 - cysA b2422 0.11 -3.1
2,537,903 - cysA b2422 0.15 -4.0
2,537,963 - cysA b2422 0.20 -5.6
2,538,033 + cysA b2422 0.27 -3.8
2,538,037 - cysA b2422 0.27 -3.3
2,538,054 - cysA b2422 0.29 -3.4
2,538,054 - cysA b2422 0.29 -4.4
2,538,084 - cysA b2422 0.31 -4.7
2,538,108 - cysA b2422 0.34 -5.4
2,538,114 + cysA b2422 0.34 -2.3
2,538,193 + cysA b2422 0.41 -2.2
2,538,193 + cysA b2422 0.41 -3.0
2,538,224 + cysA b2422 0.44 -2.6
2,538,224 + cysA b2422 0.44 -3.2
2,538,334 - cysA b2422 0.54 -4.3
2,538,334 - cysA b2422 0.54 -2.8
2,538,336 - cysA b2422 0.54 -4.2
2,538,699 + cysA b2422 0.87 -3.0
2,538,824 + -3.2
2,538,824 + -4.8
2,538,846 - -0.1
2,538,846 - -0.8
2,538,952 + cysW b2423 0.14 -3.4
2,538,952 + cysW b2423 0.14 -3.5
2,538,959 + cysW b2423 0.15 -2.7
2,538,961 + cysW b2423 0.15 -2.3
2,538,986 + cysW b2423 0.18 -3.9
2,539,072 + cysW b2423 0.28 -2.3
2,539,173 + cysW b2423 0.40 -2.0
2,539,173 + cysW b2423 0.40 -2.5
2,539,240 + cysW b2423 0.47 -3.4
2,539,249 - cysW b2423 0.48 -3.9
2,539,249 - cysW b2423 0.48 -3.4
2,539,456 + cysW b2423 0.72 -3.4
2,539,468 - cysW b2423 0.73 -1.6
2,539,468 - cysW b2423 0.73 -1.9
2,539,468 - cysW b2423 0.73 -5.1
2,539,694 - -2.2

Or see this region's nucleotide sequence