Strain Fitness in Escherichia coli BW25113 around b2311

Experiment: Ying_OrganicAcid7 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntargT and ubiX are separated by 265 nucleotidesubiX and purF are separated by 94 nucleotides b2310: argT - lysine/arginine/ornithine transporter subunit (NCBI), at 2,425,031 to 2,425,813 argT b2311: ubiX - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (NCBI), at 2,426,079 to 2,426,648 ubiX b2312: purF - amidophosphoribosyltransferase (NCBI), at 2,426,743 to 2,428,260 purF Position (kb) 2426 2427Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 2425.153 kb on + strand, within argTat 2425.153 kb on + strand, within argTat 2425.161 kb on - strand, within argTat 2425.161 kb on - strand, within argTat 2425.253 kb on + strand, within argTat 2425.280 kb on + strand, within argTat 2425.286 kb on - strand, within argTat 2425.319 kb on - strand, within argTat 2425.339 kb on - strand, within argTat 2425.353 kb on - strand, within argTat 2425.353 kb on - strand, within argTat 2425.387 kb on - strand, within argTat 2425.387 kb on - strand, within argTat 2425.402 kb on - strand, within argTat 2425.543 kb on + strand, within argTat 2425.702 kb on + strand, within argTat 2425.705 kb on - strand, within argTat 2425.732 kb on - strand, within argTat 2425.742 kb on - strandat 2425.984 kb on - strandat 2426.073 kb on - strandat 2426.795 kb on + strandat 2426.795 kb on + strandat 2427.252 kb on + strand, within purFat 2427.297 kb on - strand, within purFat 2427.537 kb on + strand, within purFat 2427.605 kb on - strand, within purF

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 3
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2,425,153 + argT b2310 0.16 +1.2
2,425,153 + argT b2310 0.16 +0.8
2,425,161 - argT b2310 0.17 +0.5
2,425,161 - argT b2310 0.17 +0.2
2,425,253 + argT b2310 0.28 +0.8
2,425,280 + argT b2310 0.32 -0.3
2,425,286 - argT b2310 0.33 -0.4
2,425,319 - argT b2310 0.37 -0.3
2,425,339 - argT b2310 0.39 +0.5
2,425,353 - argT b2310 0.41 +0.8
2,425,353 - argT b2310 0.41 +1.0
2,425,387 - argT b2310 0.45 +0.6
2,425,387 - argT b2310 0.45 +0.7
2,425,402 - argT b2310 0.47 -0.1
2,425,543 + argT b2310 0.65 +0.4
2,425,702 + argT b2310 0.86 +0.3
2,425,705 - argT b2310 0.86 +0.2
2,425,732 - argT b2310 0.90 +0.1
2,425,742 - +0.1
2,425,984 - +0.2
2,426,073 - -0.5
2,426,795 + -5.2
2,426,795 + -4.0
2,427,252 + purF b2312 0.34 -3.8
2,427,297 - purF b2312 0.36 -4.6
2,427,537 + purF b2312 0.52 -3.9
2,427,605 - purF b2312 0.57 -6.2

Or see this region's nucleotide sequence