Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ubiD and fre are separated by 45 nucleotides fre and fadA are separated by 380 nucleotides
b3843: ubiD - 3-octaprenyl-4-hydroxybenzoate decarboxylase (NCBI), at 4,023,011 to 4,024,504
ubiD
b3844: fre - NAD(P)H-flavin reductase (NCBI), at 4,024,550 to 4,025,251
fre
b3845: fadA - acetyl-CoA acetyltransferase (NCBI), at 4,025,632 to 4,026,795
fadA
Position (kb)
4024
4025
4026 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4024.500 kb on + strand at 4024.586 kb on + strand at 4024.586 kb on + strand at 4024.586 kb on - strand at 4024.586 kb on - strand at 4024.599 kb on + strand at 4024.600 kb on + strand at 4024.600 kb on + strand at 4024.608 kb on - strand at 4024.608 kb on - strand at 4024.649 kb on + strand, within fre at 4024.674 kb on - strand, within fre at 4024.693 kb on + strand, within fre at 4024.701 kb on - strand, within fre at 4024.780 kb on + strand, within fre at 4024.780 kb on + strand, within fre at 4024.801 kb on - strand, within fre at 4024.823 kb on + strand, within fre at 4024.856 kb on - strand, within fre at 4024.856 kb on - strand, within fre at 4024.891 kb on + strand, within fre at 4024.898 kb on - strand, within fre at 4024.905 kb on + strand, within fre at 4024.905 kb on + strand, within fre at 4024.968 kb on + strand, within fre at 4024.968 kb on + strand, within fre at 4024.971 kb on + strand, within fre at 4024.976 kb on - strand, within fre at 4024.994 kb on - strand, within fre at 4025.044 kb on + strand, within fre at 4025.068 kb on + strand, within fre at 4025.105 kb on + strand at 4025.132 kb on - strand, within fre at 4025.208 kb on - strand at 4025.285 kb on - strand at 4025.293 kb on + strand at 4025.307 kb on - strand at 4025.307 kb on - strand at 4025.341 kb on - strand at 4025.376 kb on - strand at 4025.376 kb on - strand at 4025.410 kb on - strand at 4025.437 kb on + strand at 4025.437 kb on + strand at 4025.442 kb on - strand at 4025.442 kb on - strand at 4025.451 kb on - strand at 4025.451 kb on - strand at 4025.507 kb on + strand at 4025.600 kb on + strand at 4025.649 kb on + strand at 4025.652 kb on + strand at 4025.653 kb on + strand at 4025.660 kb on - strand at 4025.678 kb on - strand at 4025.678 kb on - strand at 4025.678 kb on - strand at 4025.758 kb on + strand, within fadA at 4025.765 kb on + strand, within fadA at 4025.765 kb on + strand, within fadA at 4025.766 kb on - strand, within fadA at 4025.766 kb on - strand, within fadA at 4025.846 kb on + strand, within fadA at 4025.907 kb on - strand, within fadA at 4025.930 kb on + strand, within fadA at 4025.930 kb on + strand, within fadA at 4025.955 kb on - strand, within fadA at 4025.955 kb on - strand, within fadA at 4025.980 kb on - strand, within fadA at 4025.980 kb on - strand, within fadA at 4025.982 kb on + strand, within fadA at 4025.987 kb on + strand, within fadA at 4026.002 kb on - strand, within fadA at 4026.047 kb on + strand, within fadA at 4026.047 kb on + strand, within fadA at 4026.075 kb on + strand, within fadA at 4026.075 kb on + strand, within fadA at 4026.075 kb on + strand, within fadA at 4026.163 kb on + strand, within fadA at 4026.163 kb on + strand, within fadA at 4026.189 kb on + strand, within fadA at 4026.198 kb on - strand, within fadA at 4026.220 kb on + strand, within fadA
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 4,024,500 + -0.1 4,024,586 + -1.0 4,024,586 + -0.7 4,024,586 - -0.0 4,024,586 - -1.5 4,024,599 + -2.3 4,024,600 + -0.9 4,024,600 + -0.9 4,024,608 - -2.2 4,024,608 - -3.7 4,024,649 + fre b3844 0.14 -1.5 4,024,674 - fre b3844 0.18 -1.7 4,024,693 + fre b3844 0.20 -3.0 4,024,701 - fre b3844 0.22 -0.3 4,024,780 + fre b3844 0.33 -2.5 4,024,780 + fre b3844 0.33 -1.4 4,024,801 - fre b3844 0.36 -2.8 4,024,823 + fre b3844 0.39 -2.3 4,024,856 - fre b3844 0.44 -2.6 4,024,856 - fre b3844 0.44 -4.2 4,024,891 + fre b3844 0.49 +0.3 4,024,898 - fre b3844 0.50 -1.5 4,024,905 + fre b3844 0.51 -1.4 4,024,905 + fre b3844 0.51 -3.0 4,024,968 + fre b3844 0.60 -1.8 4,024,968 + fre b3844 0.60 -1.0 4,024,971 + fre b3844 0.60 -3.3 4,024,976 - fre b3844 0.61 -2.5 4,024,994 - fre b3844 0.63 -1.5 4,025,044 + fre b3844 0.70 -2.7 4,025,068 + fre b3844 0.74 -1.8 4,025,105 + -0.5 4,025,132 - fre b3844 0.83 -1.9 4,025,208 - -0.7 4,025,285 - -0.1 4,025,293 + -0.6 4,025,307 - -0.1 4,025,307 - +1.2 4,025,341 - -0.2 4,025,376 - -0.5 4,025,376 - +0.3 4,025,410 - +0.2 4,025,437 + +0.4 4,025,437 + +0.2 4,025,442 - -1.2 4,025,442 - -2.6 4,025,451 - +0.4 4,025,451 - +0.1 4,025,507 + +0.5 4,025,600 + +0.3 4,025,649 + -0.7 4,025,652 + +1.5 4,025,653 + +0.2 4,025,660 - -0.5 4,025,678 - -0.6 4,025,678 - -0.5 4,025,678 - -0.1 4,025,758 + fadA b3845 0.11 +0.5 4,025,765 + fadA b3845 0.11 +0.5 4,025,765 + fadA b3845 0.11 +0.4 4,025,766 - fadA b3845 0.12 -0.2 4,025,766 - fadA b3845 0.12 +0.5 4,025,846 + fadA b3845 0.18 -0.2 4,025,907 - fadA b3845 0.24 -1.0 4,025,930 + fadA b3845 0.26 +0.9 4,025,930 + fadA b3845 0.26 +0.9 4,025,955 - fadA b3845 0.28 -1.2 4,025,955 - fadA b3845 0.28 -1.7 4,025,980 - fadA b3845 0.30 -0.5 4,025,980 - fadA b3845 0.30 +0.4 4,025,982 + fadA b3845 0.30 -0.8 4,025,987 + fadA b3845 0.30 -0.5 4,026,002 - fadA b3845 0.32 +0.2 4,026,047 + fadA b3845 0.36 +0.3 4,026,047 + fadA b3845 0.36 -0.4 4,026,075 + fadA b3845 0.38 -0.3 4,026,075 + fadA b3845 0.38 -0.4 4,026,075 + fadA b3845 0.38 +0.1 4,026,163 + fadA b3845 0.46 +0.2 4,026,163 + fadA b3845 0.46 +0.4 4,026,189 + fadA b3845 0.48 -0.3 4,026,198 - fadA b3845 0.49 -0.5 4,026,220 + fadA b3845 0.51 -0.7
Or see this region's nucleotide sequence