Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dapF and yigA overlap by 4 nucleotides yigA and xerC overlap by 4 nucleotides xerC and yigB overlap by 1 nucleotides yigB and uvrD are separated by 83 nucleotides
b3809: dapF - diaminopimelate epimerase (VIMSS), at 3,992,785 to 3,993,609
dapF
b3810: yigA - hypothetical protein (NCBI), at 3,993,606 to 3,994,313
yigA
b3811: xerC - site-specific tyrosine recombinase XerC (NCBI), at 3,994,310 to 3,995,206
xerC
b3812: yigB - predicted hydrolase (NCBI), at 3,995,206 to 3,995,922
yigB
b3813: uvrD - DNA-dependent ATPase I and helicase II (NCBI), at 3,996,006 to 3,998,168
uvrD
Position (kb)
3994
3995
3996 Strain fitness (log2 ratio)
-2
-1
0
1 at 3993.601 kb on + strand at 3993.601 kb on + strand at 3993.637 kb on + strand at 3993.647 kb on - strand at 3993.778 kb on - strand, within yigA at 3993.778 kb on - strand, within yigA at 3993.782 kb on - strand, within yigA at 3993.813 kb on - strand, within yigA at 3993.813 kb on - strand, within yigA at 3993.815 kb on + strand, within yigA at 3993.827 kb on + strand, within yigA at 3993.827 kb on + strand, within yigA at 3993.829 kb on + strand, within yigA at 3993.837 kb on - strand, within yigA at 3993.837 kb on - strand, within yigA at 3993.854 kb on - strand, within yigA at 3993.892 kb on - strand, within yigA at 3993.892 kb on - strand, within yigA at 3993.948 kb on - strand, within yigA at 3993.948 kb on - strand, within yigA at 3993.972 kb on - strand, within yigA at 3993.978 kb on - strand, within yigA at 3994.051 kb on - strand, within yigA at 3994.088 kb on - strand, within yigA at 3994.090 kb on + strand, within yigA at 3994.090 kb on + strand, within yigA at 3994.093 kb on - strand, within yigA at 3994.098 kb on - strand, within yigA at 3994.098 kb on - strand, within yigA at 3994.098 kb on - strand, within yigA at 3994.098 kb on - strand, within yigA at 3994.098 kb on - strand, within yigA at 3994.098 kb on - strand, within yigA at 3994.170 kb on + strand, within yigA at 3994.192 kb on + strand, within yigA at 3994.192 kb on + strand, within yigA at 3994.202 kb on + strand, within yigA at 3994.210 kb on - strand, within yigA at 3994.279 kb on - strand at 3994.300 kb on - strand at 3994.300 kb on - strand at 3994.314 kb on - strand at 3994.318 kb on + strand at 3994.399 kb on + strand at 3994.765 kb on + strand, within xerC at 3994.776 kb on + strand, within xerC at 3994.932 kb on + strand, within xerC at 3995.087 kb on + strand, within xerC at 3995.093 kb on - strand, within xerC at 3995.093 kb on - strand, within xerC at 3995.095 kb on - strand, within xerC at 3995.113 kb on + strand, within xerC at 3995.165 kb on + strand at 3995.165 kb on + strand at 3995.246 kb on + strand at 3995.246 kb on + strand at 3995.251 kb on + strand at 3995.308 kb on + strand, within yigB at 3995.308 kb on + strand, within yigB at 3995.316 kb on - strand, within yigB at 3995.338 kb on + strand, within yigB at 3995.341 kb on + strand, within yigB at 3995.351 kb on - strand, within yigB at 3995.379 kb on - strand, within yigB at 3995.391 kb on - strand, within yigB at 3995.400 kb on + strand, within yigB at 3995.567 kb on + strand, within yigB at 3995.615 kb on + strand, within yigB at 3995.615 kb on + strand, within yigB at 3995.782 kb on + strand, within yigB at 3995.826 kb on - strand, within yigB at 3995.864 kb on + strand at 3995.875 kb on - strand at 3995.875 kb on - strand at 3995.950 kb on - strand at 3995.962 kb on + strand at 3996.013 kb on + strand at 3996.013 kb on + strand at 3996.013 kb on + strand at 3996.013 kb on + strand at 3996.013 kb on + strand at 3996.021 kb on - strand at 3996.037 kb on + strand at 3996.069 kb on + strand at 3996.095 kb on + strand at 3996.103 kb on + strand at 3996.111 kb on - strand at 3996.148 kb on + strand at 3996.150 kb on + strand at 3996.180 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 3,993,601 + -0.1 3,993,601 + +0.4 3,993,637 + -0.9 3,993,647 - -2.1 3,993,778 - yigA b3810 0.24 -0.3 3,993,778 - yigA b3810 0.24 -0.4 3,993,782 - yigA b3810 0.25 -0.0 3,993,813 - yigA b3810 0.29 +0.2 3,993,813 - yigA b3810 0.29 +0.6 3,993,815 + yigA b3810 0.30 -0.7 3,993,827 + yigA b3810 0.31 -1.0 3,993,827 + yigA b3810 0.31 -0.5 3,993,829 + yigA b3810 0.31 -0.1 3,993,837 - yigA b3810 0.33 -1.6 3,993,837 - yigA b3810 0.33 -0.5 3,993,854 - yigA b3810 0.35 -0.6 3,993,892 - yigA b3810 0.40 -0.4 3,993,892 - yigA b3810 0.40 -1.1 3,993,948 - yigA b3810 0.48 -0.2 3,993,948 - yigA b3810 0.48 +0.4 3,993,972 - yigA b3810 0.52 -0.2 3,993,978 - yigA b3810 0.53 -0.0 3,994,051 - yigA b3810 0.63 -0.6 3,994,088 - yigA b3810 0.68 +0.4 3,994,090 + yigA b3810 0.68 -0.4 3,994,090 + yigA b3810 0.68 +0.1 3,994,093 - yigA b3810 0.69 -0.8 3,994,098 - yigA b3810 0.69 -0.8 3,994,098 - yigA b3810 0.69 -0.7 3,994,098 - yigA b3810 0.69 -0.7 3,994,098 - yigA b3810 0.69 +0.1 3,994,098 - yigA b3810 0.69 -0.1 3,994,098 - yigA b3810 0.69 -0.9 3,994,170 + yigA b3810 0.80 -0.9 3,994,192 + yigA b3810 0.83 -0.5 3,994,192 + yigA b3810 0.83 -1.8 3,994,202 + yigA b3810 0.84 +0.6 3,994,210 - yigA b3810 0.85 -0.2 3,994,279 - -0.0 3,994,300 - -1.0 3,994,300 - -2.4 3,994,314 - -0.5 3,994,318 + +0.0 3,994,399 + +0.8 3,994,765 + xerC b3811 0.51 -2.0 3,994,776 + xerC b3811 0.52 +0.9 3,994,932 + xerC b3811 0.69 +0.6 3,995,087 + xerC b3811 0.87 +0.9 3,995,093 - xerC b3811 0.87 +0.9 3,995,093 - xerC b3811 0.87 -1.5 3,995,095 - xerC b3811 0.88 +0.9 3,995,113 + xerC b3811 0.90 +1.7 3,995,165 + -0.9 3,995,165 + +1.7 3,995,246 + -0.6 3,995,246 + +0.3 3,995,251 + -0.4 3,995,308 + yigB b3812 0.14 +0.2 3,995,308 + yigB b3812 0.14 +0.1 3,995,316 - yigB b3812 0.15 +0.0 3,995,338 + yigB b3812 0.18 +0.4 3,995,341 + yigB b3812 0.19 +0.1 3,995,351 - yigB b3812 0.20 -0.0 3,995,379 - yigB b3812 0.24 -0.4 3,995,391 - yigB b3812 0.26 -0.0 3,995,400 + yigB b3812 0.27 -0.5 3,995,567 + yigB b3812 0.50 -0.2 3,995,615 + yigB b3812 0.57 -1.0 3,995,615 + yigB b3812 0.57 -0.7 3,995,782 + yigB b3812 0.80 +0.1 3,995,826 - yigB b3812 0.86 +0.2 3,995,864 + +0.4 3,995,875 - -0.6 3,995,875 - +0.6 3,995,950 - +0.1 3,995,962 + -0.0 3,996,013 + +0.7 3,996,013 + +0.2 3,996,013 + -0.4 3,996,013 + +0.6 3,996,013 + -0.6 3,996,021 - -0.0 3,996,037 + -0.5 3,996,069 + -1.0 3,996,095 + -0.2 3,996,103 + -0.3 3,996,111 - -2.1 3,996,148 + +0.4 3,996,150 + -0.0 3,996,180 - -0.5
Or see this region's nucleotide sequence