Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rhlB and trxA are separated by 130 nucleotides trxA and rho are separated by 326 nucleotides
b3780: rhlB - ATP-dependent RNA helicase (NCBI), at 3,962,388 to 3,963,653
rhlB
b3781: trxA - thioredoxin 1 (VIMSS), at 3,963,784 to 3,964,113
trxA
b3783: rho - transcription termination factor Rho (NCBI), at 3,964,440 to 3,965,699
rho
Position (kb)
3963
3964
3965 Strain fitness (log2 ratio)
-2
-1
0
1 at 3962.803 kb on - strand, within rhlB at 3962.811 kb on - strand, within rhlB at 3962.822 kb on + strand, within rhlB at 3962.840 kb on + strand, within rhlB at 3962.863 kb on - strand, within rhlB at 3962.863 kb on - strand, within rhlB at 3962.889 kb on - strand, within rhlB at 3962.942 kb on + strand, within rhlB at 3962.957 kb on + strand, within rhlB at 3962.957 kb on + strand, within rhlB at 3963.061 kb on + strand, within rhlB at 3963.110 kb on - strand, within rhlB at 3963.117 kb on - strand, within rhlB at 3963.120 kb on + strand, within rhlB at 3963.139 kb on + strand, within rhlB at 3963.139 kb on + strand, within rhlB at 3963.149 kb on + strand, within rhlB at 3963.196 kb on - strand, within rhlB at 3963.196 kb on - strand, within rhlB at 3963.207 kb on + strand, within rhlB at 3963.265 kb on + strand, within rhlB at 3963.265 kb on + strand, within rhlB at 3963.268 kb on - strand, within rhlB at 3963.275 kb on + strand, within rhlB at 3963.288 kb on - strand, within rhlB at 3963.295 kb on + strand, within rhlB at 3963.295 kb on + strand, within rhlB at 3963.346 kb on - strand, within rhlB at 3963.417 kb on - strand, within rhlB at 3963.417 kb on - strand, within rhlB at 3963.445 kb on - strand, within rhlB at 3963.450 kb on - strand, within rhlB at 3963.503 kb on - strand, within rhlB at 3963.513 kb on - strand, within rhlB at 3963.542 kb on + strand at 3963.542 kb on + strand at 3963.550 kb on - strand at 3963.553 kb on + strand at 3963.553 kb on + strand at 3963.561 kb on - strand at 3963.561 kb on - strand at 3963.605 kb on - strand at 3963.632 kb on - strand at 3963.655 kb on + strand at 3963.655 kb on + strand at 3963.694 kb on - strand at 3963.758 kb on - strand at 3963.764 kb on - strand at 3963.769 kb on - strand at 3963.780 kb on + strand at 3963.788 kb on - strand at 3963.788 kb on - strand at 3963.829 kb on + strand at 3963.837 kb on - strand, within trxA at 3963.839 kb on + strand, within trxA at 3963.856 kb on - strand, within trxA at 3963.957 kb on + strand, within trxA at 3963.975 kb on + strand, within trxA at 3963.975 kb on + strand, within trxA at 3963.975 kb on + strand, within trxA at 3964.021 kb on - strand, within trxA at 3964.026 kb on - strand, within trxA at 3964.068 kb on + strand, within trxA at 3964.068 kb on + strand at 3964.080 kb on + strand, within trxA at 3964.110 kb on - strand at 3964.110 kb on - strand at 3964.155 kb on + strand at 3964.179 kb on + strand at 3964.254 kb on + strand at 3964.308 kb on + strand at 3964.318 kb on - strand at 3964.320 kb on + strand at 3964.320 kb on + strand at 3964.320 kb on + strand at 3964.355 kb on + strand at 3964.378 kb on + strand at 3964.386 kb on - strand at 3964.460 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 3,962,803 - rhlB b3780 0.33 +1.3 3,962,811 - rhlB b3780 0.33 +0.9 3,962,822 + rhlB b3780 0.34 +0.1 3,962,840 + rhlB b3780 0.36 -0.7 3,962,863 - rhlB b3780 0.38 -0.3 3,962,863 - rhlB b3780 0.38 +0.0 3,962,889 - rhlB b3780 0.40 +0.4 3,962,942 + rhlB b3780 0.44 +0.1 3,962,957 + rhlB b3780 0.45 -0.6 3,962,957 + rhlB b3780 0.45 -1.9 3,963,061 + rhlB b3780 0.53 -0.0 3,963,110 - rhlB b3780 0.57 +1.1 3,963,117 - rhlB b3780 0.58 +0.3 3,963,120 + rhlB b3780 0.58 +0.3 3,963,139 + rhlB b3780 0.59 -0.3 3,963,139 + rhlB b3780 0.59 -0.6 3,963,149 + rhlB b3780 0.60 -0.4 3,963,196 - rhlB b3780 0.64 -0.4 3,963,196 - rhlB b3780 0.64 +0.6 3,963,207 + rhlB b3780 0.65 -0.2 3,963,265 + rhlB b3780 0.69 +0.6 3,963,265 + rhlB b3780 0.69 +0.3 3,963,268 - rhlB b3780 0.70 -0.1 3,963,275 + rhlB b3780 0.70 +0.5 3,963,288 - rhlB b3780 0.71 -0.5 3,963,295 + rhlB b3780 0.72 +0.2 3,963,295 + rhlB b3780 0.72 -0.5 3,963,346 - rhlB b3780 0.76 +0.7 3,963,417 - rhlB b3780 0.81 -1.4 3,963,417 - rhlB b3780 0.81 +0.9 3,963,445 - rhlB b3780 0.83 +0.7 3,963,450 - rhlB b3780 0.84 +0.2 3,963,503 - rhlB b3780 0.88 +0.3 3,963,513 - rhlB b3780 0.89 +0.1 3,963,542 + +0.8 3,963,542 + +0.4 3,963,550 - -0.2 3,963,553 + -0.1 3,963,553 + +0.4 3,963,561 - +0.2 3,963,561 - +0.3 3,963,605 - +0.3 3,963,632 - +1.4 3,963,655 + +0.7 3,963,655 + +0.1 3,963,694 - -1.3 3,963,758 - -0.7 3,963,764 - -0.7 3,963,769 - -0.6 3,963,780 + -1.1 3,963,788 - -1.3 3,963,788 - -0.1 3,963,829 + +0.8 3,963,837 - trxA b3781 0.16 +0.0 3,963,839 + trxA b3781 0.17 -1.3 3,963,856 - trxA b3781 0.22 -1.2 3,963,957 + trxA b3781 0.52 -1.3 3,963,975 + trxA b3781 0.58 -1.1 3,963,975 + trxA b3781 0.58 -1.7 3,963,975 + trxA b3781 0.58 -0.0 3,964,021 - trxA b3781 0.72 -0.3 3,964,026 - trxA b3781 0.73 -1.3 3,964,068 + trxA b3781 0.86 -0.6 3,964,068 + -0.4 3,964,080 + trxA b3781 0.90 -0.3 3,964,110 - -0.8 3,964,110 - -1.6 3,964,155 + +0.5 3,964,179 + +0.2 3,964,254 + -0.5 3,964,308 + +0.9 3,964,318 - -1.7 3,964,320 + -0.6 3,964,320 + -0.2 3,964,320 + +0.0 3,964,355 + -0.1 3,964,378 + -0.2 3,964,386 - +0.1 3,964,460 + -1.5
Or see this region's nucleotide sequence