Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mdh and argR are separated by 434 nucleotides argR and yhcN are separated by 364 nucleotides yhcN and yhcO are separated by 55 nucleotides
b3236: mdh - malate dehydrogenase (NCBI), at 3,381,352 to 3,382,290
mdh
b3237: argR - arginine repressor (NCBI), at 3,382,725 to 3,383,195
argR
b3238: yhcN - orf, hypothetical protein (VIMSS), at 3,383,560 to 3,383,823
yhcN
b3239: yhcO - predicted barnase inhibitor (NCBI), at 3,383,879 to 3,384,151
yhcO
Position (kb)
3382
3383
3384 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 3381.729 kb on + strand, within mdh at 3381.761 kb on - strand, within mdh at 3381.909 kb on - strand, within mdh at 3381.909 kb on - strand, within mdh at 3382.001 kb on + strand, within mdh at 3382.021 kb on - strand, within mdh at 3382.031 kb on - strand, within mdh at 3382.031 kb on - strand, within mdh at 3382.033 kb on + strand, within mdh at 3382.033 kb on + strand, within mdh at 3382.079 kb on - strand, within mdh at 3382.149 kb on + strand, within mdh at 3382.163 kb on - strand, within mdh at 3382.187 kb on + strand, within mdh at 3382.193 kb on + strand, within mdh at 3382.193 kb on + strand, within mdh at 3382.444 kb on + strand at 3382.465 kb on + strand at 3382.515 kb on - strand at 3382.656 kb on - strand at 3382.656 kb on - strand at 3382.689 kb on + strand at 3382.709 kb on - strand at 3382.722 kb on + strand at 3382.730 kb on - strand at 3382.730 kb on - strand at 3382.737 kb on + strand at 3382.739 kb on - strand at 3382.754 kb on + strand at 3382.829 kb on - strand, within argR at 3382.829 kb on - strand, within argR at 3382.888 kb on - strand, within argR at 3382.941 kb on - strand, within argR at 3382.941 kb on - strand, within argR at 3382.955 kb on - strand, within argR at 3382.957 kb on + strand, within argR at 3383.008 kb on - strand, within argR at 3383.024 kb on - strand, within argR at 3383.039 kb on + strand, within argR at 3383.059 kb on + strand, within argR at 3383.063 kb on + strand, within argR at 3383.118 kb on - strand, within argR at 3383.118 kb on - strand, within argR at 3383.137 kb on - strand, within argR at 3383.153 kb on - strand at 3383.236 kb on - strand at 3383.249 kb on - strand at 3383.250 kb on - strand at 3383.430 kb on + strand at 3383.430 kb on + strand at 3383.466 kb on + strand at 3383.524 kb on - strand at 3383.524 kb on - strand at 3383.546 kb on - strand at 3383.573 kb on + strand at 3383.650 kb on - strand, within yhcN at 3383.650 kb on - strand, within yhcN at 3383.790 kb on + strand, within yhcN at 3383.833 kb on + strand at 3383.833 kb on + strand at 3383.903 kb on + strand at 3383.903 kb on + strand at 3384.023 kb on + strand, within yhcO at 3384.032 kb on + strand, within yhcO at 3384.042 kb on - strand, within yhcO at 3384.042 kb on - strand, within yhcO at 3384.042 kb on - strand, within yhcO at 3384.193 kb on + strand at 3384.193 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 3,381,729 + mdh b3236 0.40 -5.5 3,381,761 - mdh b3236 0.44 -4.8 3,381,909 - mdh b3236 0.59 -2.8 3,381,909 - mdh b3236 0.59 -3.5 3,382,001 + mdh b3236 0.69 -5.3 3,382,021 - mdh b3236 0.71 -4.0 3,382,031 - mdh b3236 0.72 -2.4 3,382,031 - mdh b3236 0.72 -2.7 3,382,033 + mdh b3236 0.73 -4.1 3,382,033 + mdh b3236 0.73 -2.2 3,382,079 - mdh b3236 0.77 -4.7 3,382,149 + mdh b3236 0.85 -4.8 3,382,163 - mdh b3236 0.86 -2.6 3,382,187 + mdh b3236 0.89 -4.6 3,382,193 + mdh b3236 0.90 -2.6 3,382,193 + mdh b3236 0.90 -3.4 3,382,444 + -1.1 3,382,465 + -0.5 3,382,515 - -0.0 3,382,656 - +0.0 3,382,656 - +0.2 3,382,689 + +0.7 3,382,709 - -1.4 3,382,722 + -3.2 3,382,730 - -1.9 3,382,730 - -2.3 3,382,737 + -2.4 3,382,739 - -1.8 3,382,754 + -2.4 3,382,829 - argR b3237 0.22 -2.4 3,382,829 - argR b3237 0.22 -2.6 3,382,888 - argR b3237 0.35 -2.4 3,382,941 - argR b3237 0.46 -0.9 3,382,941 - argR b3237 0.46 -1.3 3,382,955 - argR b3237 0.49 -1.2 3,382,957 + argR b3237 0.49 -2.3 3,383,008 - argR b3237 0.60 -3.2 3,383,024 - argR b3237 0.63 -1.3 3,383,039 + argR b3237 0.67 -2.1 3,383,059 + argR b3237 0.71 -2.7 3,383,063 + argR b3237 0.72 -2.6 3,383,118 - argR b3237 0.83 -1.7 3,383,118 - argR b3237 0.83 -2.6 3,383,137 - argR b3237 0.87 -3.7 3,383,153 - -3.2 3,383,236 - +0.3 3,383,249 - +0.8 3,383,250 - +0.1 3,383,430 + +0.4 3,383,430 + -1.1 3,383,466 + -0.1 3,383,524 - -0.1 3,383,524 - -0.3 3,383,546 - -0.2 3,383,573 + -0.3 3,383,650 - yhcN b3238 0.34 +1.2 3,383,650 - yhcN b3238 0.34 +2.8 3,383,790 + yhcN b3238 0.87 -0.2 3,383,833 + +0.5 3,383,833 + +0.3 3,383,903 + -0.3 3,383,903 + -0.7 3,384,023 + yhcO b3239 0.53 +0.5 3,384,032 + yhcO b3239 0.56 +0.0 3,384,042 - yhcO b3239 0.60 +0.5 3,384,042 - yhcO b3239 0.60 +0.1 3,384,042 - yhcO b3239 0.60 +0.8 3,384,193 + -0.2 3,384,193 + -0.2
Or see this region's nucleotide sequence