Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nlpI and pnp are separated by 108 nucleotides pnp and sraG are separated by 56 nucleotides sraG and rpsO are separated by 16 nucleotides rpsO and truB are separated by 148 nucleotides
b3163: nlpI - hypothetical protein (NCBI), at 3,306,062 to 3,306,946
nlpI
b3164: pnp - polynucleotide phosphorylase/polyadenylase (RefSeq), at 3,307,055 to 3,309,190
pnp
b4449: sraG - unknown RNA (NCBI), at 3,309,247 to 3,309,420
sraG
b3165: rpsO - 30S ribosomal protein S15 (NCBI), at 3,309,437 to 3,309,706
rpsO
b3166: truB - tRNA pseudouridine synthase B (NCBI), at 3,309,855 to 3,310,799
truB
Position (kb)
3307
3308
3309
3310 Strain fitness (log2 ratio)
-2
-1
0
1 at 3306.073 kb on - strand at 3306.103 kb on - strand at 3306.171 kb on + strand, within nlpI at 3306.189 kb on - strand, within nlpI at 3306.189 kb on - strand, within nlpI at 3306.192 kb on - strand, within nlpI at 3306.216 kb on - strand, within nlpI at 3306.275 kb on - strand, within nlpI at 3306.275 kb on - strand, within nlpI at 3306.640 kb on - strand, within nlpI at 3306.640 kb on - strand, within nlpI at 3306.652 kb on - strand, within nlpI at 3306.676 kb on + strand, within nlpI at 3306.712 kb on + strand, within nlpI at 3306.728 kb on + strand, within nlpI at 3306.729 kb on + strand, within nlpI at 3306.855 kb on - strand, within nlpI at 3307.056 kb on + strand at 3307.056 kb on + strand at 3307.069 kb on - strand at 3307.069 kb on - strand at 3307.074 kb on - strand at 3307.095 kb on - strand at 3307.117 kb on + strand at 3307.200 kb on - strand at 3307.247 kb on - strand at 3307.279 kb on - strand, within pnp at 3307.314 kb on - strand, within pnp at 3307.354 kb on - strand, within pnp at 3307.359 kb on - strand, within pnp at 3307.371 kb on - strand, within pnp at 3307.471 kb on - strand, within pnp at 3309.216 kb on - strand at 3309.239 kb on - strand at 3309.437 kb on - strand at 3309.823 kb on - strand at 3309.823 kb on - strand at 3309.838 kb on - strand at 3309.987 kb on - strand, within truB at 3309.990 kb on - strand, within truB at 3310.065 kb on + strand, within truB at 3310.085 kb on - strand, within truB at 3310.126 kb on + strand, within truB at 3310.148 kb on + strand, within truB at 3310.148 kb on + strand, within truB
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 3,306,073 - -1.7 3,306,103 - -1.3 3,306,171 + nlpI b3163 0.12 -0.8 3,306,189 - nlpI b3163 0.14 -0.8 3,306,189 - nlpI b3163 0.14 -0.6 3,306,192 - nlpI b3163 0.15 +0.1 3,306,216 - nlpI b3163 0.17 -0.0 3,306,275 - nlpI b3163 0.24 -0.4 3,306,275 - nlpI b3163 0.24 +0.0 3,306,640 - nlpI b3163 0.65 -1.7 3,306,640 - nlpI b3163 0.65 -0.1 3,306,652 - nlpI b3163 0.67 -1.2 3,306,676 + nlpI b3163 0.69 +0.1 3,306,712 + nlpI b3163 0.73 -2.6 3,306,728 + nlpI b3163 0.75 -0.9 3,306,729 + nlpI b3163 0.75 -1.1 3,306,855 - nlpI b3163 0.90 -0.5 3,307,056 + -0.8 3,307,056 + -0.8 3,307,069 - -0.4 3,307,069 - -0.7 3,307,074 - -0.3 3,307,095 - -0.0 3,307,117 + +1.4 3,307,200 - -0.1 3,307,247 - -0.7 3,307,279 - pnp b3164 0.10 -2.0 3,307,314 - pnp b3164 0.12 +0.2 3,307,354 - pnp b3164 0.14 -1.5 3,307,359 - pnp b3164 0.14 -0.7 3,307,371 - pnp b3164 0.15 -2.1 3,307,471 - pnp b3164 0.19 -1.3 3,309,216 - +0.6 3,309,239 - -0.2 3,309,437 - +0.4 3,309,823 - -0.6 3,309,823 - +0.1 3,309,838 - -0.9 3,309,987 - truB b3166 0.14 +0.3 3,309,990 - truB b3166 0.14 +0.5 3,310,065 + truB b3166 0.22 +0.1 3,310,085 - truB b3166 0.24 +0.7 3,310,126 + truB b3166 0.29 +0.4 3,310,148 + truB b3166 0.31 -0.1 3,310,148 + truB b3166 0.31 -0.1
Or see this region's nucleotide sequence