Strain Fitness in Escherichia coli BW25113 around b2853

Experiment: Ying_AminoAcid20 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygeH and ygeI are separated by 167 nucleotidesygeI and pbl are separated by 82 nucleotidespbl and ygeK are separated by 18 nucleotides b2852: ygeH - predictedtranscriptional regulator (NCBI), at 2,990,116 to 2,991,492 ygeH b2853: ygeI - hypothetical protein (NCBI), at 2,991,660 to 2,991,878 ygeI b2854: pbl - orf, hypothetical protein (VIMSS), at 2,991,961 to 2,992,463 pbl b2856: ygeK - hypothetical protein (RefSeq), at 2,992,482 to 2,993,114 ygeK Position (kb) 2991 2992Strain fitness (log2 ratio) -1 0 1 2at 2991.764 kb on + strand, within ygeIat 2991.764 kb on + strand, within ygeIat 2992.046 kb on - strand, within pblat 2992.531 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3
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2,991,764 + ygeI b2853 0.47 +0.1
2,991,764 + ygeI b2853 0.47 +1.1
2,992,046 - pbl b2854 0.17 +2.7
2,992,531 - +2.7

Or see this region's nucleotide sequence