Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purM and purN overlap by 1 nucleotides purN and ppk are separated by 171 nucleotides ppk and ppx are separated by 4 nucleotides
b2499: purM - phosphoribosylaminoimidazole synthetase (NCBI), at 2,619,219 to 2,620,256
purM
b2500: purN - phosphoribosylglycinamide formyltransferase (NCBI), at 2,620,256 to 2,620,894
purN
b2501: ppk - polyphosphate kinase (NCBI), at 2,621,066 to 2,623,132
ppk
b2502: ppx - exopolyphosphatase (NCBI), at 2,623,137 to 2,624,678
ppx
Position (kb)
2621
2622
2623
2624 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 2620.164 kb on + strand at 2620.164 kb on + strand at 2620.164 kb on + strand at 2620.175 kb on + strand at 2620.258 kb on + strand at 2620.258 kb on + strand at 2620.260 kb on + strand at 2620.422 kb on - strand, within purN at 2620.451 kb on + strand, within purN at 2620.459 kb on - strand, within purN at 2620.470 kb on - strand, within purN at 2620.604 kb on + strand, within purN at 2620.651 kb on + strand, within purN at 2620.651 kb on + strand, within purN at 2620.661 kb on - strand, within purN at 2620.744 kb on + strand, within purN at 2620.873 kb on + strand at 2620.873 kb on + strand at 2620.899 kb on - strand at 2620.981 kb on + strand at 2620.981 kb on + strand at 2620.989 kb on - strand at 2620.989 kb on - strand at 2620.998 kb on - strand at 2621.024 kb on - strand at 2621.032 kb on - strand at 2621.041 kb on - strand at 2621.118 kb on - strand at 2621.325 kb on - strand, within ppk at 2621.325 kb on - strand, within ppk at 2621.444 kb on + strand, within ppk at 2621.452 kb on + strand, within ppk at 2621.521 kb on + strand, within ppk at 2621.521 kb on + strand, within ppk at 2621.586 kb on - strand, within ppk at 2621.692 kb on - strand, within ppk at 2621.786 kb on - strand, within ppk at 2621.786 kb on - strand, within ppk at 2621.808 kb on + strand, within ppk at 2621.840 kb on - strand, within ppk at 2621.840 kb on - strand, within ppk at 2621.850 kb on + strand, within ppk at 2621.850 kb on + strand, within ppk at 2621.869 kb on + strand, within ppk at 2621.869 kb on + strand, within ppk at 2621.928 kb on - strand, within ppk at 2622.104 kb on - strand, within ppk at 2622.131 kb on - strand, within ppk at 2622.137 kb on - strand, within ppk at 2622.151 kb on - strand, within ppk at 2622.151 kb on - strand, within ppk at 2622.248 kb on - strand, within ppk at 2622.268 kb on - strand, within ppk at 2622.324 kb on + strand, within ppk at 2622.329 kb on + strand, within ppk at 2622.345 kb on + strand, within ppk at 2622.388 kb on - strand, within ppk at 2622.461 kb on + strand, within ppk at 2622.461 kb on + strand, within ppk at 2622.471 kb on - strand, within ppk at 2622.550 kb on - strand, within ppk at 2622.628 kb on - strand, within ppk at 2622.647 kb on + strand, within ppk at 2622.647 kb on + strand, within ppk at 2622.667 kb on - strand, within ppk at 2622.667 kb on - strand, within ppk at 2622.702 kb on - strand, within ppk at 2622.702 kb on - strand, within ppk at 2622.720 kb on - strand, within ppk at 2622.729 kb on - strand, within ppk at 2622.782 kb on - strand, within ppk at 2622.839 kb on - strand, within ppk at 2622.897 kb on - strand, within ppk at 2622.923 kb on - strand, within ppk at 2623.010 kb on + strand at 2623.037 kb on - strand at 2623.112 kb on - strand at 2623.112 kb on - strand at 2623.135 kb on - strand at 2623.171 kb on + strand at 2623.220 kb on - strand at 2623.220 kb on - strand at 2623.304 kb on - strand, within ppx at 2623.395 kb on - strand, within ppx at 2623.398 kb on - strand, within ppx at 2623.429 kb on + strand, within ppx at 2623.532 kb on - strand, within ppx at 2623.677 kb on + strand, within ppx at 2623.677 kb on + strand, within ppx at 2623.720 kb on + strand, within ppx at 2623.720 kb on + strand, within ppx at 2623.755 kb on + strand, within ppx at 2623.755 kb on + strand, within ppx at 2623.756 kb on - strand, within ppx at 2623.769 kb on - strand, within ppx at 2623.769 kb on - strand, within ppx at 2623.941 kb on + strand, within ppx at 2623.941 kb on + strand, within ppx at 2623.942 kb on - strand, within ppx at 2623.996 kb on + strand, within ppx at 2624.019 kb on - strand, within ppx at 2624.040 kb on - strand at 2624.040 kb on - strand, within ppx at 2624.119 kb on - strand, within ppx at 2624.127 kb on + strand, within ppx
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 2,620,164 + -3.6 2,620,164 + -2.2 2,620,164 + -3.5 2,620,175 + -3.1 2,620,258 + +0.0 2,620,258 + +0.1 2,620,260 + -4.8 2,620,422 - purN b2500 0.26 -5.9 2,620,451 + purN b2500 0.31 -2.6 2,620,459 - purN b2500 0.32 -5.6 2,620,470 - purN b2500 0.33 -5.1 2,620,604 + purN b2500 0.54 -4.2 2,620,651 + purN b2500 0.62 -3.4 2,620,651 + purN b2500 0.62 -4.2 2,620,661 - purN b2500 0.63 -4.0 2,620,744 + purN b2500 0.76 -3.9 2,620,873 + +0.0 2,620,873 + +0.3 2,620,899 - +2.7 2,620,981 + +0.5 2,620,981 + +0.2 2,620,989 - -0.1 2,620,989 - +0.5 2,620,998 - +0.9 2,621,024 - +0.3 2,621,032 - -1.9 2,621,041 - -0.0 2,621,118 - -3.9 2,621,325 - ppk b2501 0.13 -1.1 2,621,325 - ppk b2501 0.13 -2.5 2,621,444 + ppk b2501 0.18 -3.2 2,621,452 + ppk b2501 0.19 -3.4 2,621,521 + ppk b2501 0.22 -1.4 2,621,521 + ppk b2501 0.22 -1.9 2,621,586 - ppk b2501 0.25 -1.5 2,621,692 - ppk b2501 0.30 -2.7 2,621,786 - ppk b2501 0.35 -2.0 2,621,786 - ppk b2501 0.35 -2.8 2,621,808 + ppk b2501 0.36 -0.7 2,621,840 - ppk b2501 0.37 -2.2 2,621,840 - ppk b2501 0.37 -0.5 2,621,850 + ppk b2501 0.38 -1.0 2,621,850 + ppk b2501 0.38 -3.5 2,621,869 + ppk b2501 0.39 -0.5 2,621,869 + ppk b2501 0.39 +0.5 2,621,928 - ppk b2501 0.42 -0.9 2,622,104 - ppk b2501 0.50 -3.0 2,622,131 - ppk b2501 0.52 -2.4 2,622,137 - ppk b2501 0.52 -2.4 2,622,151 - ppk b2501 0.52 -1.5 2,622,151 - ppk b2501 0.52 -2.4 2,622,248 - ppk b2501 0.57 -1.6 2,622,268 - ppk b2501 0.58 -4.0 2,622,324 + ppk b2501 0.61 -1.4 2,622,329 + ppk b2501 0.61 -4.2 2,622,345 + ppk b2501 0.62 -4.0 2,622,388 - ppk b2501 0.64 -2.8 2,622,461 + ppk b2501 0.67 -3.2 2,622,461 + ppk b2501 0.67 -2.2 2,622,471 - ppk b2501 0.68 -4.9 2,622,550 - ppk b2501 0.72 -1.9 2,622,628 - ppk b2501 0.76 -3.3 2,622,647 + ppk b2501 0.76 -2.8 2,622,647 + ppk b2501 0.76 -1.7 2,622,667 - ppk b2501 0.77 -2.2 2,622,667 - ppk b2501 0.77 -0.5 2,622,702 - ppk b2501 0.79 -2.8 2,622,702 - ppk b2501 0.79 -0.2 2,622,720 - ppk b2501 0.80 -0.2 2,622,729 - ppk b2501 0.80 -5.1 2,622,782 - ppk b2501 0.83 -4.0 2,622,839 - ppk b2501 0.86 -2.7 2,622,897 - ppk b2501 0.89 -2.3 2,622,923 - ppk b2501 0.90 -2.0 2,623,010 + -2.1 2,623,037 - -2.0 2,623,112 - -0.5 2,623,112 - +0.4 2,623,135 - -0.6 2,623,171 + +0.8 2,623,220 - +0.4 2,623,220 - +1.6 2,623,304 - ppx b2502 0.11 -0.3 2,623,395 - ppx b2502 0.17 -0.0 2,623,398 - ppx b2502 0.17 +0.5 2,623,429 + ppx b2502 0.19 +0.3 2,623,532 - ppx b2502 0.26 +0.2 2,623,677 + ppx b2502 0.35 -0.2 2,623,677 + ppx b2502 0.35 +0.8 2,623,720 + ppx b2502 0.38 +0.8 2,623,720 + ppx b2502 0.38 -0.8 2,623,755 + ppx b2502 0.40 +0.5 2,623,755 + ppx b2502 0.40 +1.0 2,623,756 - ppx b2502 0.40 +1.0 2,623,769 - ppx b2502 0.41 +0.9 2,623,769 - ppx b2502 0.41 -0.0 2,623,941 + ppx b2502 0.52 +0.7 2,623,941 + ppx b2502 0.52 +0.5 2,623,942 - ppx b2502 0.52 +0.8 2,623,996 + ppx b2502 0.56 +0.7 2,624,019 - ppx b2502 0.57 +0.7 2,624,040 - +0.5 2,624,040 - ppx b2502 0.59 +0.3 2,624,119 - ppx b2502 0.64 -0.1 2,624,127 + ppx b2502 0.64 +0.1
Or see this region's nucleotide sequence