Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nuoL and nuoK overlap by 4 nucleotides nuoK and nuoJ overlap by 4 nucleotides nuoJ and nuoI are separated by 11 nucleotides nuoI and nuoH are separated by 14 nucleotides
b2278: nuoL - NADH dehydrogenase subunit L (NCBI), at 2,391,227 to 2,393,068
nuoL
b2279: nuoK - NADH dehydrogenase subunit K (NCBI), at 2,393,065 to 2,393,367
nuoK
b2280: nuoJ - NADH dehydrogenase subunit J (NCBI), at 2,393,364 to 2,393,918
nuoJ
b2281: nuoI - NADH dehydrogenase subunit I (NCBI), at 2,393,930 to 2,394,472
nuoI
b2282: nuoH - NADH dehydrogenase subunit H (NCBI), at 2,394,487 to 2,395,464
nuoH
Position (kb)
2393
2394 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2392.403 kb on - strand, within nuoL at 2392.403 kb on - strand, within nuoL at 2392.419 kb on - strand, within nuoL at 2392.424 kb on - strand, within nuoL at 2392.488 kb on - strand, within nuoL at 2392.498 kb on - strand, within nuoL at 2392.537 kb on - strand, within nuoL at 2392.555 kb on - strand, within nuoL at 2392.578 kb on + strand, within nuoL at 2392.593 kb on + strand, within nuoL at 2392.593 kb on + strand, within nuoL at 2392.593 kb on + strand, within nuoL at 2392.601 kb on - strand, within nuoL at 2392.601 kb on - strand, within nuoL at 2392.601 kb on - strand, within nuoL at 2392.620 kb on + strand, within nuoL at 2392.627 kb on + strand, within nuoL at 2392.694 kb on - strand, within nuoL at 2392.723 kb on + strand, within nuoL at 2392.723 kb on + strand, within nuoL at 2392.728 kb on + strand, within nuoL at 2392.728 kb on + strand, within nuoL at 2392.771 kb on + strand, within nuoL at 2392.776 kb on + strand, within nuoL at 2392.831 kb on - strand, within nuoL at 2392.831 kb on - strand, within nuoL at 2392.831 kb on - strand, within nuoL at 2392.831 kb on - strand, within nuoL at 2392.874 kb on - strand, within nuoL at 2392.895 kb on - strand at 2393.077 kb on + strand at 2393.077 kb on + strand at 2393.106 kb on + strand, within nuoK at 2393.152 kb on - strand, within nuoK at 2393.213 kb on - strand, within nuoK at 2393.323 kb on + strand, within nuoK at 2393.346 kb on + strand at 2393.410 kb on + strand at 2393.412 kb on + strand at 2393.412 kb on + strand at 2393.419 kb on + strand at 2393.456 kb on - strand, within nuoJ at 2393.463 kb on - strand, within nuoJ at 2393.530 kb on - strand, within nuoJ at 2393.530 kb on - strand, within nuoJ at 2393.536 kb on + strand, within nuoJ at 2393.559 kb on + strand, within nuoJ at 2393.581 kb on - strand, within nuoJ at 2393.586 kb on - strand, within nuoJ at 2393.606 kb on + strand, within nuoJ at 2393.611 kb on - strand, within nuoJ at 2393.617 kb on + strand, within nuoJ at 2393.637 kb on + strand, within nuoJ at 2393.638 kb on + strand, within nuoJ at 2393.638 kb on + strand, within nuoJ at 2393.645 kb on + strand, within nuoJ at 2393.724 kb on + strand, within nuoJ at 2393.731 kb on - strand, within nuoJ at 2393.839 kb on - strand, within nuoJ at 2393.840 kb on + strand, within nuoJ at 2393.876 kb on + strand at 2393.898 kb on - strand at 2393.915 kb on + strand at 2393.915 kb on + strand at 2393.967 kb on - strand at 2393.967 kb on - strand at 2394.043 kb on + strand, within nuoI at 2394.124 kb on - strand, within nuoI at 2394.163 kb on + strand, within nuoI at 2394.320 kb on + strand, within nuoI at 2394.328 kb on - strand, within nuoI at 2394.345 kb on - strand, within nuoI at 2394.347 kb on - strand, within nuoI at 2394.411 kb on - strand, within nuoI at 2394.482 kb on - strand at 2394.495 kb on - strand at 2394.495 kb on - strand at 2394.603 kb on + strand, within nuoH at 2394.603 kb on + strand, within nuoH at 2394.624 kb on + strand, within nuoH at 2394.634 kb on - strand, within nuoH at 2394.712 kb on - strand at 2394.804 kb on + strand, within nuoH at 2394.815 kb on + strand, within nuoH at 2394.834 kb on + strand, within nuoH
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 2,392,403 - nuoL b2278 0.64 -1.9 2,392,403 - nuoL b2278 0.64 -2.5 2,392,419 - nuoL b2278 0.65 -2.5 2,392,424 - nuoL b2278 0.65 -2.9 2,392,488 - nuoL b2278 0.68 -3.1 2,392,498 - nuoL b2278 0.69 -3.7 2,392,537 - nuoL b2278 0.71 -0.4 2,392,555 - nuoL b2278 0.72 -2.4 2,392,578 + nuoL b2278 0.73 -2.2 2,392,593 + nuoL b2278 0.74 +0.3 2,392,593 + nuoL b2278 0.74 -1.7 2,392,593 + nuoL b2278 0.74 -2.8 2,392,601 - nuoL b2278 0.75 -2.6 2,392,601 - nuoL b2278 0.75 -2.9 2,392,601 - nuoL b2278 0.75 -1.4 2,392,620 + nuoL b2278 0.76 -3.7 2,392,627 + nuoL b2278 0.76 -2.1 2,392,694 - nuoL b2278 0.80 -2.3 2,392,723 + nuoL b2278 0.81 -1.4 2,392,723 + nuoL b2278 0.81 -1.8 2,392,728 + nuoL b2278 0.81 -1.6 2,392,728 + nuoL b2278 0.81 -2.5 2,392,771 + nuoL b2278 0.84 -4.1 2,392,776 + nuoL b2278 0.84 -3.3 2,392,831 - nuoL b2278 0.87 -2.3 2,392,831 - nuoL b2278 0.87 -3.5 2,392,831 - nuoL b2278 0.87 -3.9 2,392,831 - nuoL b2278 0.87 -2.8 2,392,874 - nuoL b2278 0.89 -5.3 2,392,895 - -5.0 2,393,077 + -1.6 2,393,077 + -2.6 2,393,106 + nuoK b2279 0.14 -0.9 2,393,152 - nuoK b2279 0.29 -2.7 2,393,213 - nuoK b2279 0.49 -2.0 2,393,323 + nuoK b2279 0.85 -3.7 2,393,346 + -1.8 2,393,410 + -2.4 2,393,412 + -4.0 2,393,412 + -2.7 2,393,419 + -2.4 2,393,456 - nuoJ b2280 0.17 -0.6 2,393,463 - nuoJ b2280 0.18 -3.2 2,393,530 - nuoJ b2280 0.30 -4.1 2,393,530 - nuoJ b2280 0.30 -3.3 2,393,536 + nuoJ b2280 0.31 -2.1 2,393,559 + nuoJ b2280 0.35 -2.7 2,393,581 - nuoJ b2280 0.39 -0.4 2,393,586 - nuoJ b2280 0.40 -4.1 2,393,606 + nuoJ b2280 0.44 -3.6 2,393,611 - nuoJ b2280 0.45 -2.9 2,393,617 + nuoJ b2280 0.46 -2.7 2,393,637 + nuoJ b2280 0.49 -3.3 2,393,638 + nuoJ b2280 0.49 -1.9 2,393,638 + nuoJ b2280 0.49 -2.8 2,393,645 + nuoJ b2280 0.51 -1.8 2,393,724 + nuoJ b2280 0.65 -3.0 2,393,731 - nuoJ b2280 0.66 -3.6 2,393,839 - nuoJ b2280 0.86 -2.2 2,393,840 + nuoJ b2280 0.86 -2.7 2,393,876 + -2.7 2,393,898 - -2.8 2,393,915 + -2.2 2,393,915 + -0.5 2,393,967 - -2.7 2,393,967 - -4.5 2,394,043 + nuoI b2281 0.21 -1.0 2,394,124 - nuoI b2281 0.36 -2.6 2,394,163 + nuoI b2281 0.43 -3.5 2,394,320 + nuoI b2281 0.72 -2.2 2,394,328 - nuoI b2281 0.73 -2.3 2,394,345 - nuoI b2281 0.76 -2.9 2,394,347 - nuoI b2281 0.77 -0.4 2,394,411 - nuoI b2281 0.89 -2.9 2,394,482 - -2.0 2,394,495 - +0.3 2,394,495 - -0.5 2,394,603 + nuoH b2282 0.12 -1.9 2,394,603 + nuoH b2282 0.12 -2.2 2,394,624 + nuoH b2282 0.14 -2.6 2,394,634 - nuoH b2282 0.15 -3.7 2,394,712 - -0.4 2,394,804 + nuoH b2282 0.32 -3.4 2,394,815 + nuoH b2282 0.34 -2.3 2,394,834 + nuoH b2282 0.35 -2.5
Or see this region's nucleotide sequence