Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybgC and tolQ overlap by 4 nucleotides tolQ and tolR are separated by 3 nucleotides tolR and tolA are separated by 64 nucleotides
b0736: ybgC - predicted acyl-CoA thioesterase (NCBI), at 773,975 to 774,379
ybgC
b0737: tolQ - membrane spanning protein in TolA-TolQ-TolR complex (NCBI), at 774,376 to 775,068
tolQ
b0738: tolR - membrane spanning protein in TolA-TolQ-TolR complex (NCBI), at 775,072 to 775,500
tolR
b0739: tolA - cell envelope integrity inner membrane protein TolA (NCBI), at 775,565 to 776,830
tolA
Position (kb) 775
776 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 774.103 kb on + strand, within ybgC at 774.103 kb on + strand, within ybgC at 774.256 kb on + strand, within ybgC at 774.265 kb on - strand, within ybgC at 774.299 kb on - strand, within ybgC at 774.336 kb on + strand, within ybgC at 774.390 kb on - strand at 774.561 kb on + strand, within tolQ at 774.571 kb on + strand, within tolQ at 774.649 kb on + strand, within tolQ at 774.668 kb on - strand, within tolQ at 774.723 kb on + strand, within tolQ at 774.723 kb on + strand, within tolQ at 774.809 kb on - strand, within tolQ at 774.813 kb on - strand, within tolQ at 774.818 kb on - strand, within tolQ at 774.839 kb on + strand, within tolQ at 774.905 kb on + strand, within tolQ at 775.063 kb on + strand at 775.063 kb on + strand at 775.070 kb on - strand at 775.071 kb on - strand at 775.071 kb on - strand at 775.091 kb on - strand at 775.121 kb on + strand, within tolR at 775.121 kb on + strand, within tolR at 775.121 kb on + strand, within tolR at 775.146 kb on + strand, within tolR at 775.152 kb on - strand, within tolR at 775.194 kb on - strand, within tolR at 775.258 kb on + strand, within tolR at 775.265 kb on + strand, within tolR at 775.275 kb on + strand, within tolR at 775.280 kb on + strand, within tolR at 775.288 kb on - strand, within tolR at 775.288 kb on - strand, within tolR at 775.298 kb on - strand, within tolR at 775.333 kb on + strand, within tolR at 775.333 kb on + strand, within tolR at 775.357 kb on - strand, within tolR at 775.526 kb on - strand at 775.533 kb on + strand at 775.565 kb on + strand at 775.628 kb on + strand at 775.651 kb on + strand at 775.659 kb on - strand at 775.672 kb on + strand at 775.748 kb on + strand, within tolA at 775.753 kb on + strand, within tolA at 775.840 kb on + strand, within tolA at 775.930 kb on - strand, within tolA at 775.932 kb on + strand, within tolA at 775.947 kb on + strand, within tolA at 776.098 kb on - strand, within tolA at 776.124 kb on + strand, within tolA at 776.146 kb on + strand, within tolA at 776.265 kb on - strand, within tolA at 776.265 kb on - strand, within tolA at 776.464 kb on + strand, within tolA at 776.475 kb on - strand, within tolA
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 774,103 + ybgC b0736 0.32 +0.7 774,103 + ybgC b0736 0.32 +0.0 774,256 + ybgC b0736 0.69 +0.3 774,265 - ybgC b0736 0.72 -1.5 774,299 - ybgC b0736 0.80 -2.9 774,336 + ybgC b0736 0.89 +1.0 774,390 - -2.7 774,561 + tolQ b0737 0.27 -1.1 774,571 + tolQ b0737 0.28 -3.4 774,649 + tolQ b0737 0.39 -1.8 774,668 - tolQ b0737 0.42 -1.5 774,723 + tolQ b0737 0.50 -3.6 774,723 + tolQ b0737 0.50 -1.6 774,809 - tolQ b0737 0.62 -3.2 774,813 - tolQ b0737 0.63 -3.1 774,818 - tolQ b0737 0.64 -1.8 774,839 + tolQ b0737 0.67 -1.8 774,905 + tolQ b0737 0.76 -2.7 775,063 + +0.1 775,063 + -0.3 775,070 - +0.2 775,071 - -0.9 775,071 - -1.9 775,091 - -1.2 775,121 + tolR b0738 0.11 -1.3 775,121 + tolR b0738 0.11 -2.1 775,121 + tolR b0738 0.11 -1.4 775,146 + tolR b0738 0.17 -3.1 775,152 - tolR b0738 0.19 -2.3 775,194 - tolR b0738 0.28 -2.2 775,258 + tolR b0738 0.43 -1.7 775,265 + tolR b0738 0.45 -0.1 775,275 + tolR b0738 0.47 -0.3 775,280 + tolR b0738 0.48 -2.9 775,288 - tolR b0738 0.50 -1.8 775,288 - tolR b0738 0.50 -1.2 775,298 - tolR b0738 0.53 -2.2 775,333 + tolR b0738 0.61 -3.5 775,333 + tolR b0738 0.61 -2.1 775,357 - tolR b0738 0.66 -0.7 775,526 - -1.3 775,533 + +0.2 775,565 + +0.2 775,628 + -2.2 775,651 + -3.2 775,659 - -1.1 775,672 + -3.6 775,748 + tolA b0739 0.14 -3.4 775,753 + tolA b0739 0.15 -0.8 775,840 + tolA b0739 0.22 -0.5 775,930 - tolA b0739 0.29 -2.5 775,932 + tolA b0739 0.29 -3.6 775,947 + tolA b0739 0.30 -3.0 776,098 - tolA b0739 0.42 -2.3 776,124 + tolA b0739 0.44 -1.8 776,146 + tolA b0739 0.46 -1.0 776,265 - tolA b0739 0.55 -3.2 776,265 - tolA b0739 0.55 -0.3 776,464 + tolA b0739 0.71 -3.3 776,475 - tolA b0739 0.72 -1.4
Or see this region's nucleotide sequence