Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sdhC and sdhD overlap by 7 nucleotides sdhD and sdhA overlap by 1 nucleotides
b0721: sdhC - succinate dehydrogenase cytochrome b556 large membrane subunit (NCBI), at 754,400 to 754,789
sdhC
b0722: sdhD - succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI), at 754,783 to 755,130
sdhD
b0723: sdhA - succinate dehydrogenase flavoprotein subunit (NCBI), at 755,130 to 756,896
sdhA
Position (kb) 754
755
756 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 753.813 kb on + strand at 753.846 kb on + strand at 753.944 kb on + strand at 754.017 kb on - strand at 754.126 kb on + strand at 754.174 kb on + strand at 754.209 kb on + strand at 754.305 kb on + strand at 754.305 kb on + strand at 754.385 kb on + strand at 754.477 kb on - strand, within sdhC at 754.507 kb on - strand, within sdhC at 754.512 kb on + strand, within sdhC at 754.550 kb on + strand, within sdhC at 754.636 kb on - strand, within sdhC at 754.663 kb on - strand, within sdhC at 754.715 kb on + strand, within sdhC at 754.759 kb on - strand at 754.775 kb on - strand at 754.802 kb on + strand at 754.809 kb on + strand at 754.909 kb on - strand, within sdhD at 754.950 kb on + strand, within sdhD at 754.966 kb on - strand, within sdhD at 754.977 kb on + strand, within sdhD at 754.990 kb on + strand, within sdhD at 755.014 kb on - strand, within sdhD at 755.019 kb on + strand, within sdhD at 755.045 kb on + strand, within sdhD at 755.083 kb on - strand, within sdhD at 755.098 kb on + strand at 755.115 kb on + strand at 755.115 kb on + strand at 755.116 kb on - strand at 755.290 kb on + strand at 755.301 kb on + strand at 755.320 kb on + strand, within sdhA at 755.323 kb on - strand, within sdhA at 755.344 kb on - strand, within sdhA at 755.353 kb on + strand, within sdhA at 755.366 kb on - strand, within sdhA at 755.368 kb on - strand, within sdhA at 755.382 kb on + strand, within sdhA at 755.424 kb on + strand, within sdhA at 755.424 kb on + strand, within sdhA at 755.461 kb on - strand, within sdhA at 755.461 kb on - strand, within sdhA at 755.481 kb on - strand, within sdhA at 755.491 kb on - strand, within sdhA at 755.554 kb on + strand, within sdhA at 755.562 kb on + strand, within sdhA at 755.562 kb on + strand, within sdhA at 755.562 kb on + strand, within sdhA at 755.564 kb on + strand, within sdhA at 755.570 kb on - strand, within sdhA at 755.570 kb on - strand, within sdhA at 755.570 kb on - strand, within sdhA at 755.572 kb on + strand, within sdhA at 755.625 kb on + strand, within sdhA at 755.626 kb on - strand, within sdhA at 755.658 kb on + strand, within sdhA at 755.658 kb on + strand, within sdhA at 755.663 kb on + strand, within sdhA at 755.676 kb on - strand, within sdhA at 755.676 kb on - strand, within sdhA at 755.676 kb on - strand, within sdhA at 755.697 kb on - strand, within sdhA at 755.711 kb on + strand, within sdhA at 755.759 kb on + strand, within sdhA at 755.759 kb on + strand, within sdhA at 755.764 kb on + strand, within sdhA at 755.764 kb on + strand, within sdhA at 755.800 kb on + strand, within sdhA at 755.839 kb on + strand at 755.842 kb on - strand, within sdhA at 755.961 kb on - strand, within sdhA
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 753,813 + -1.9 753,846 + -1.5 753,944 + -0.1 754,017 - +0.1 754,126 + -0.1 754,174 + +0.5 754,209 + -0.6 754,305 + -1.3 754,305 + -1.3 754,385 + -1.3 754,477 - sdhC b0721 0.20 -4.1 754,507 - sdhC b0721 0.27 -3.8 754,512 + sdhC b0721 0.29 -3.2 754,550 + sdhC b0721 0.38 -4.5 754,636 - sdhC b0721 0.61 -4.2 754,663 - sdhC b0721 0.67 -4.1 754,715 + sdhC b0721 0.81 -0.1 754,759 - -4.1 754,775 - -4.7 754,802 + -2.1 754,809 + -2.3 754,909 - sdhD b0722 0.36 -4.2 754,950 + sdhD b0722 0.48 -2.1 754,966 - sdhD b0722 0.53 -3.3 754,977 + sdhD b0722 0.56 -2.6 754,990 + sdhD b0722 0.59 -2.6 755,014 - sdhD b0722 0.66 -3.1 755,019 + sdhD b0722 0.68 -2.9 755,045 + sdhD b0722 0.75 -1.5 755,083 - sdhD b0722 0.86 -2.4 755,098 + -0.6 755,115 + -0.7 755,115 + -0.9 755,116 - -3.4 755,290 + -1.8 755,301 + -2.3 755,320 + sdhA b0723 0.11 -3.7 755,323 - sdhA b0723 0.11 -3.8 755,344 - sdhA b0723 0.12 -2.8 755,353 + sdhA b0723 0.13 -3.7 755,366 - sdhA b0723 0.13 -2.9 755,368 - sdhA b0723 0.13 -3.6 755,382 + sdhA b0723 0.14 -3.9 755,424 + sdhA b0723 0.17 -3.9 755,424 + sdhA b0723 0.17 -3.8 755,461 - sdhA b0723 0.19 -4.0 755,461 - sdhA b0723 0.19 -2.8 755,481 - sdhA b0723 0.20 -2.9 755,491 - sdhA b0723 0.20 -4.4 755,554 + sdhA b0723 0.24 -1.7 755,562 + sdhA b0723 0.24 -2.9 755,562 + sdhA b0723 0.24 -2.9 755,562 + sdhA b0723 0.24 -2.8 755,564 + sdhA b0723 0.25 -3.0 755,570 - sdhA b0723 0.25 -3.2 755,570 - sdhA b0723 0.25 -4.2 755,570 - sdhA b0723 0.25 -4.5 755,572 + sdhA b0723 0.25 -1.6 755,625 + sdhA b0723 0.28 -2.3 755,626 - sdhA b0723 0.28 -0.8 755,658 + sdhA b0723 0.30 -1.7 755,658 + sdhA b0723 0.30 -3.0 755,663 + sdhA b0723 0.30 -3.2 755,676 - sdhA b0723 0.31 -4.0 755,676 - sdhA b0723 0.31 -3.6 755,676 - sdhA b0723 0.31 -5.2 755,697 - sdhA b0723 0.32 -4.1 755,711 + sdhA b0723 0.33 -2.5 755,759 + sdhA b0723 0.36 -1.8 755,759 + sdhA b0723 0.36 -1.8 755,764 + sdhA b0723 0.36 -4.5 755,764 + sdhA b0723 0.36 -4.6 755,800 + sdhA b0723 0.38 -3.1 755,839 + +0.3 755,842 - sdhA b0723 0.40 -5.4 755,961 - sdhA b0723 0.47 -2.4
Or see this region's nucleotide sequence