Strain Fitness in Escherichia coli BW25113 around b3843

Experiment: Ying_AminoAcid20 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttatD and rfaH overlap by 4 nucleotidesrfaH and ubiD are separated by 166 nucleotidesubiD and fre are separated by 45 nucleotides b4483: tatD - DNase, magnesium-dependent (NCBI), at 4,021,577 to 4,022,359 tatD b3842: rfaH - transcriptional activator RfaH (NCBI), at 4,022,356 to 4,022,844 rfaH b3843: ubiD - 3-octaprenyl-4-hydroxybenzoate decarboxylase (NCBI), at 4,023,011 to 4,024,504 ubiD b3844: fre - NAD(P)H-flavin reductase (NCBI), at 4,024,550 to 4,025,251 fre Position (kb) 4023 4024 4025Strain fitness (log2 ratio) -3 -2 -1 0 1at 4022.055 kb on + strand, within tatDat 4022.055 kb on + strand, within tatDat 4022.056 kb on - strand, within tatDat 4022.124 kb on + strand, within tatDat 4022.191 kb on + strand, within tatDat 4022.223 kb on + strand, within tatDat 4022.252 kb on - strand, within tatDat 4022.261 kb on + strand, within tatDat 4022.271 kb on + strand, within tatDat 4022.271 kb on + strand, within tatDat 4022.271 kb on + strand, within tatDat 4022.271 kb on + strand, within tatDat 4022.284 kb on + strandat 4022.284 kb on + strandat 4022.284 kb on + strandat 4022.284 kb on - strandat 4022.293 kb on + strandat 4022.294 kb on + strandat 4022.306 kb on - strandat 4022.306 kb on - strandat 4022.327 kb on - strandat 4022.339 kb on - strandat 4022.353 kb on + strandat 4022.353 kb on + strandat 4022.376 kb on - strandat 4022.376 kb on - strandat 4022.379 kb on + strandat 4022.379 kb on + strandat 4022.387 kb on - strandat 4022.389 kb on - strandat 4022.399 kb on - strandat 4022.424 kb on + strand, within rfaHat 4022.424 kb on + strand, within rfaHat 4022.431 kb on - strand, within rfaHat 4022.434 kb on + strand, within rfaHat 4022.437 kb on - strand, within rfaHat 4022.495 kb on - strand, within rfaHat 4022.500 kb on - strand, within rfaHat 4022.500 kb on - strand, within rfaHat 4022.557 kb on + strand, within rfaHat 4022.559 kb on + strand, within rfaHat 4022.563 kb on + strand, within rfaHat 4022.585 kb on + strand, within rfaHat 4022.624 kb on - strand, within rfaHat 4022.634 kb on + strand, within rfaHat 4022.650 kb on - strand, within rfaHat 4022.688 kb on - strand, within rfaHat 4022.701 kb on + strand, within rfaHat 4022.701 kb on + strand, within rfaHat 4022.709 kb on + strand, within rfaHat 4022.759 kb on + strand, within rfaHat 4022.762 kb on - strand, within rfaHat 4022.764 kb on + strand, within rfaHat 4022.765 kb on + strand, within rfaHat 4022.777 kb on + strand, within rfaHat 4022.793 kb on - strand, within rfaHat 4022.813 kb on + strandat 4022.816 kb on + strandat 4022.824 kb on + strandat 4022.849 kb on + strandat 4022.857 kb on - strandat 4022.884 kb on - strandat 4022.905 kb on - strandat 4022.920 kb on + strandat 4022.980 kb on + strandat 4022.980 kb on + strandat 4022.986 kb on + strandat 4022.996 kb on + strandat 4022.996 kb on + strandat 4022.996 kb on + strandat 4022.996 kb on + strandat 4023.004 kb on - strandat 4023.004 kb on - strandat 4023.037 kb on + strandat 4023.037 kb on + strandat 4023.043 kb on - strandat 4024.500 kb on + strandat 4024.586 kb on + strandat 4024.586 kb on + strandat 4024.586 kb on - strandat 4024.586 kb on - strandat 4024.599 kb on + strandat 4024.600 kb on + strandat 4024.600 kb on + strandat 4024.608 kb on - strandat 4024.608 kb on - strandat 4024.649 kb on + strand, within freat 4024.674 kb on - strand, within freat 4024.693 kb on + strand, within freat 4024.701 kb on - strand, within freat 4024.780 kb on + strand, within freat 4024.780 kb on + strand, within freat 4024.801 kb on - strand, within freat 4024.823 kb on + strand, within freat 4024.856 kb on - strand, within freat 4024.856 kb on - strand, within freat 4024.891 kb on + strand, within freat 4024.898 kb on - strand, within freat 4024.905 kb on + strand, within freat 4024.905 kb on + strand, within freat 4024.968 kb on + strand, within freat 4024.968 kb on + strand, within freat 4024.971 kb on + strand, within freat 4024.976 kb on - strand, within freat 4024.994 kb on - strand, within freat 4025.044 kb on + strand, within freat 4025.068 kb on + strand, within freat 4025.105 kb on + strandat 4025.132 kb on - strand, within freat 4025.208 kb on - strandat 4025.285 kb on - strandat 4025.293 kb on + strandat 4025.307 kb on - strandat 4025.307 kb on - strandat 4025.341 kb on - strandat 4025.376 kb on - strandat 4025.376 kb on - strandat 4025.410 kb on - strandat 4025.437 kb on + strandat 4025.437 kb on + strandat 4025.442 kb on - strandat 4025.442 kb on - strandat 4025.451 kb on - strandat 4025.451 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3
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4,022,055 + tatD b4483 0.61 +0.2
4,022,055 + tatD b4483 0.61 +0.1
4,022,056 - tatD b4483 0.61 -0.4
4,022,124 + tatD b4483 0.70 +0.6
4,022,191 + tatD b4483 0.78 +0.3
4,022,223 + tatD b4483 0.83 +0.3
4,022,252 - tatD b4483 0.86 +0.6
4,022,261 + tatD b4483 0.87 +0.2
4,022,271 + tatD b4483 0.89 +1.7
4,022,271 + tatD b4483 0.89 -0.2
4,022,271 + tatD b4483 0.89 +0.0
4,022,271 + tatD b4483 0.89 -0.0
4,022,284 + -0.2
4,022,284 + +0.2
4,022,284 + -0.3
4,022,284 - +0.1
4,022,293 + -0.3
4,022,294 + +0.3
4,022,306 - +0.3
4,022,306 - +0.0
4,022,327 - -0.8
4,022,339 - -0.1
4,022,353 + -0.2
4,022,353 + +0.2
4,022,376 - -0.5
4,022,376 - -0.4
4,022,379 + +0.1
4,022,379 + -0.2
4,022,387 - +0.2
4,022,389 - -0.5
4,022,399 - -0.0
4,022,424 + rfaH b3842 0.14 -0.6
4,022,424 + rfaH b3842 0.14 -0.4
4,022,431 - rfaH b3842 0.15 +1.1
4,022,434 + rfaH b3842 0.16 +0.1
4,022,437 - rfaH b3842 0.17 +0.4
4,022,495 - rfaH b3842 0.28 -0.7
4,022,500 - rfaH b3842 0.29 -0.1
4,022,500 - rfaH b3842 0.29 -0.3
4,022,557 + rfaH b3842 0.41 +0.6
4,022,559 + rfaH b3842 0.42 -0.2
4,022,563 + rfaH b3842 0.42 -0.3
4,022,585 + rfaH b3842 0.47 -0.1
4,022,624 - rfaH b3842 0.55 -1.0
4,022,634 + rfaH b3842 0.57 +0.7
4,022,650 - rfaH b3842 0.60 -0.7
4,022,688 - rfaH b3842 0.68 +0.4
4,022,701 + rfaH b3842 0.71 -0.3
4,022,701 + rfaH b3842 0.71 -0.5
4,022,709 + rfaH b3842 0.72 +0.8
4,022,759 + rfaH b3842 0.82 -0.4
4,022,762 - rfaH b3842 0.83 +0.1
4,022,764 + rfaH b3842 0.83 +0.3
4,022,765 + rfaH b3842 0.84 +0.4
4,022,777 + rfaH b3842 0.86 -0.1
4,022,793 - rfaH b3842 0.89 +0.5
4,022,813 + +0.4
4,022,816 + +0.7
4,022,824 + +0.2
4,022,849 + -0.2
4,022,857 - -0.1
4,022,884 - +0.0
4,022,905 - +0.7
4,022,920 + -0.9
4,022,980 + +0.2
4,022,980 + -0.7
4,022,986 + -1.1
4,022,996 + +0.3
4,022,996 + +0.4
4,022,996 + -0.1
4,022,996 + +0.2
4,023,004 - -0.0
4,023,004 - +0.2
4,023,037 + -1.3
4,023,037 + +0.6
4,023,043 - -0.2
4,024,500 + -0.5
4,024,586 + -0.8
4,024,586 + -1.5
4,024,586 - -1.9
4,024,586 - -1.3
4,024,599 + -2.1
4,024,600 + -0.7
4,024,600 + +0.3
4,024,608 - -3.0
4,024,608 - -1.9
4,024,649 + fre b3844 0.14 -1.8
4,024,674 - fre b3844 0.18 -3.1
4,024,693 + fre b3844 0.20 -2.2
4,024,701 - fre b3844 0.22 -0.1
4,024,780 + fre b3844 0.33 -1.7
4,024,780 + fre b3844 0.33 -2.2
4,024,801 - fre b3844 0.36 -0.6
4,024,823 + fre b3844 0.39 -0.5
4,024,856 - fre b3844 0.44 -1.9
4,024,856 - fre b3844 0.44 -3.0
4,024,891 + fre b3844 0.49 +0.5
4,024,898 - fre b3844 0.50 -1.8
4,024,905 + fre b3844 0.51 -1.2
4,024,905 + fre b3844 0.51 -1.9
4,024,968 + fre b3844 0.60 -2.6
4,024,968 + fre b3844 0.60 -0.8
4,024,971 + fre b3844 0.60 -3.1
4,024,976 - fre b3844 0.61 -3.3
4,024,994 - fre b3844 0.63 -1.3
4,025,044 + fre b3844 0.70 -3.6
4,025,068 + fre b3844 0.74 -2.2
4,025,105 + -0.4
4,025,132 - fre b3844 0.83 -1.1
4,025,208 - -0.9
4,025,285 - +0.1
4,025,293 + +0.6
4,025,307 - -1.7
4,025,307 - +0.5
4,025,341 - -0.2
4,025,376 - +0.4
4,025,376 - -0.2
4,025,410 - +0.1
4,025,437 + -1.1
4,025,437 + +0.3
4,025,442 - -1.4
4,025,442 - -0.8
4,025,451 - -0.1
4,025,451 - -0.1

Or see this region's nucleotide sequence